Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Geobacillus thermoleovorans | |
2-mercaptoethanol | - |
Geobacillus thermoleovorans | |
bestatin | - |
Geobacillus thermoleovorans | |
DTT | - |
Geobacillus thermoleovorans | |
EDTA | - |
Geobacillus thermoleovorans | |
iodoacetate | - |
Geobacillus thermoleovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.658 | - |
L-Leu-4-nitroanilide | pH 7.6-7.8, 60°C | Geobacillus thermoleovorans | |
25 | - |
L-Leu-Gly-Gly | pH 7.6-7.8, 60°C | Geobacillus thermoleovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | reactivates the apoenzyme | Geobacillus thermoleovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42977 | - |
1 * 42977, mass spectrometry | Geobacillus thermoleovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermoleovorans | - |
- |
- |
Geobacillus thermoleovorans 47b | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 164fold to homogeneity by ammonium sulfate fractionation, ion exchange and hydroxyapatite chromatography, again ion exchange chromatography, and gel filtration, followed by again ion exchange and hydroxyapatite chromatography | Geobacillus thermoleovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Geobacillus thermoleovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Arg-4-nitroanilide + H2O | - |
Geobacillus thermoleovorans | L-Arg + 4-nitroaniline | - |
? | |
L-Arg-4-nitroanilide + H2O | - |
Geobacillus thermoleovorans 47b | L-Arg + 4-nitroaniline | - |
? | |
L-Leu-4-nitroanilide + H2O | - |
Geobacillus thermoleovorans | L-Leu + 4-nitroaniline | - |
? | |
L-Leu-4-nitroanilide + H2O | - |
Geobacillus thermoleovorans 47b | L-Leu + 4-nitroaniline | - |
? | |
L-Leu-Gly-Gly + H2O | - |
Geobacillus thermoleovorans | L-Leu + Gly-Gly | - |
? | |
L-Leu-Gly-Gly + H2O | - |
Geobacillus thermoleovorans 47b | L-Leu + Gly-Gly | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42977, mass spectrometry | Geobacillus thermoleovorans |
Synonyms | Comment | Organism |
---|---|---|
LAP | - |
Geobacillus thermoleovorans |
leucine aminopeptidase | - |
Geobacillus thermoleovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Geobacillus thermoleovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
1 h, stable up to | Geobacillus thermoleovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10180 | - |
L-Leu-4-nitroanilide | pH 7.6-7.8, 60°C | Geobacillus thermoleovorans | |
49540 | - |
L-Leu-Gly-Gly | pH 7.6-7.8, 60°C | Geobacillus thermoleovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | 7.8 | - |
Geobacillus thermoleovorans |