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Literature summary for 3.4.11.10 extracted from
- Preparation of carboxylated magnetic particles for the efficient immobilization of C-terminally lysine-tagged Bacillus stearothermophilus aminopeptidase II (2010), J. Ind. Microbiol. Biotechnol., 37, 717-735.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and modified Lys-tagged enzymes in Escherichia coli strain M15 | Geobacillus stearothermophilus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | preparation of C-terminally Lys-tagged aminopeptidase II, with either tri- or nona-lysines, and immobilization of the modified enzymes on carboxylated magnetic particles, method and evaluation of enzyme properties, overview. The carboxylated magnetic particles are prepared by the simple co-precipitation of Fe3+/Fe2+ in aqueous medium and then subsequently modified with adipic acid. The immobilized enzymes, especially BsAPII-Lys9, can be recovered magnetically and used repeatedly, thermal and storage stabilities of the immobilized BsAPII-Lys9 are greatly improved | Geobacillus stearothermophilus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Geobacillus stearothermophilus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44500 | - |
x * 44500, recombinant enzyme, SDS-PAGE | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and modified Lys-tagged enzymes from Escherichia coli strain M15 | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activities of free and immobilized recombinant Lys-tagged enzymes, overview | Geobacillus stearothermophilus |
| 10.41 | - |
free recombinant wild-type enzyme | Geobacillus stearothermophilus |
| 15.86 | - |
immobilized recombinant wild-type enzyme | Geobacillus stearothermophilus |
| 18.22 | - |
free recombinant Lys9-tagged enzyme | Geobacillus stearothermophilus |
| 21.71 | - |
free recombinant Lys3-tagged enzyme | Geobacillus stearothermophilus |
| 60.73 | - |
immobilized recombinant Lys3-tagged enzyme | Geobacillus stearothermophilus |
| 76.91 | - |
immobilized recombinant Lys9-tagged enzyme | Geobacillus stearothermophilus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 30 days, residual activity of free BsAPII, BsAPII-Lys3, and BsAPII-Lys9 is 69.4%, 74.5%, and 71.7%, respectively. Bound enzymes also retain more than 61.2% of the original activity, storage stability of BsAPII, BsAPII-Lys3, and BsAPII-Lys9 reamins unaltered after binding to magnetic particles, overview | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-leucyl-4-nitroanilide + H2O | - |
Geobacillus stearothermophilus | L-leucine + 4-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44500, recombinant enzyme, SDS-PAGE | Geobacillus stearothermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aminopeptidase II | - |
Geobacillus stearothermophilus |
| APII | - |
Geobacillus stearothermophilus |
| LAPII | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Geobacillus stearothermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | activity range, profiles of free and immobilized recombinant wild-type and mutant enzymes, overview | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | stability range, profiles of free and immobilized recombinant wild-type and mutant enzymes, overview | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10.5 | activity range, profiles of free and immobilized recombinant wild-type and mutant enzymes, overview | Geobacillus stearothermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10.5 | stability range, profiles of free and immobilized recombinant wild-type and mutant enzymes, overview | Geobacillus stearothermophilus |
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