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Literature summary for 3.4.11.2 extracted from

  • Yoshimoto, T.; Tamesa, Y.; Gushi, K.; Murayama, N.; Tsuru, D.
    An aminopeptidase N from Escherichia coli HB1010: Purification and demonstration that the enzyme possesses arylamidase and peptidase activities (1988), Agric. Biol. Chem., 52, 217-225.
No PubMed abstract available
Search for more literature for 3.4.11.2

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Escherichia coli
4-chloromercuribenzoate
-
 Escherichia coli
amastatin
-
 Escherichia coli
bestatin
-
 Escherichia coli
Cu2+
-
 Escherichia coli
Hg2+
-
 Escherichia coli
puromycin
-
 Escherichia coli
Zn2+
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 Leu-2-naphthylamide
-
 Escherichia coli
0.74
-
 Gly-2-naphthylamide
-
 Escherichia coli
1
-
 Ala-2-naphthylamide
-
 Escherichia coli
2.86
-
 Ala-p-nitroanilide
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ reactivation after inhibition by 1,10-phenanthroline Escherichia coli
Zn2+ at concentrations between 0.1 and 0.3 mM reactivation after inhibition by 1,10-phenanthroline, inhibitory at higher concentrations Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 gel filtration, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
aminoacyl peptide Escherichia coli
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.78
-
 Gly-2-naphthylamide as substrate Escherichia coli
1.6
-
 Met-2-naphthylamide as substrate Escherichia coli
1.68
-
 Ala-p-nitroanilide as substrate Escherichia coli
3.54
-
 Ala-2-naphthylamide as substrate Escherichia coli
4.8
-
 Leu-2-naphthylamide as substrate Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-2-naphthylamide + H2O
-
 Escherichia coli Ala + 2-naphthylamine
-
 ?
Ala-p-nitroanilide + H2O
-
 Escherichia coli Ala + p-nitroaniline
-
 ?
aminoacyl peptide
-
 Escherichia coli ?
-
 ?
dipeptides + H2O not: Leu-Pro Escherichia coli amino acids
-
 ?
Gly-2-naphthylamide + H2O
-
 Escherichia coli Gly + 2-naphthylamine
-
 ?
Leu-2-naphthylamide + H2O
-
 Escherichia coli Leu + 2-naphthylamine
-
 ?
Leu-Gly + H2O
-
 Escherichia coli Leu + Gly
-
 ?
Met-2-naphthylamide + H2O
-
 Escherichia coli Met + 2-naphthylamine
-
 ?
tripeptides + H2O not: Gly-Pro-Ala Escherichia coli dipeptides + amino acids
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 80000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 activity was measured for 5 min Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 9 stable for 1h at 30°C Escherichia coli