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Literature summary for 3.4.11.21 extracted from

  • Yokoyama, R.; Kawasaki, H.; Hirano, H.
    Identification of yeast aspartyl aminopeptidase gene by purifying and characterizing its product from yeast cells (2006), FEBS J., 273, 192-198.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene YHR113W, located on chromosome VIII, DNA and amino acid sequence determination and analysis Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 33.5% inhibition at 5 mM Saccharomyces cerevisiae
EDTA 76.1% inhibition at 20 mM Saccharomyces cerevisiae
additional information poor inhibition by bestatin at 0.2 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.064
-
angiotensin I
-
Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
monomer, determined by SDS-PAGE Saccharomyces cerevisiae
680000
-
gel filtration Saccharomyces cerevisiae
680000
-
native enzyme, dodecamer, determined using a Superose 6 column Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae B-8032
-
-
-

Purification (Commentary)

Purification (Comment) Organism
aspartyl aminopeptidase is purified from yeast cells, using ultracentrifugation, ammonium sulfate fractionating, and chromatography on a Bio-Gel column, on a Mono Q column, and a Superose 6 column Saccharomyces cerevisiae
native enzyme to homogeneity by ultracentrifugation, ammonium sulfate fractionation, anion exchange chromatography, and gel filtration Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
angiotensin I: 100%, angiotensin II: 144%, angiotensinogen 1-14: 35%, angiotensin II antipeptide: 116%, N-acetyl-angiotensinogen 1-14: 0%, Tyr-bradykinin: 0%, Ile-Ser-bradykinin: 0%, [sar1,ala8] angiotensin II: 0% Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin I + H2O
-
Saccharomyces cerevisiae ?
-
?
angiotensin I + H2O
-
Saccharomyces cerevisiae B-8032 ?
-
?
angiotensin I + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu Saccharomyces cerevisiae Asp + Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu
-
?
angiotensin I + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu Saccharomyces cerevisiae B-8032 Asp + Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu
-
?
angiotensin II + H2O
-
Saccharomyces cerevisiae ?
-
?
angiotensin II + H2O
-
Saccharomyces cerevisiae B-8032 ?
-
?
angiotensin II + H2O i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe, best substrate Saccharomyces cerevisiae Asp + angiotensin III i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe ?
angiotensin II + H2O i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe, best substrate Saccharomyces cerevisiae B-8032 Asp + angiotensin III i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe ?
angiotensin II antipeptide + H2O i.e. Glu-Gly-Val-Thr-Val-His-Pro-Val Saccharomyces cerevisiae Glu + Gly-Val-Thr-Val-His-Pro-Val
-
?
angiotensin II antipeptide + H2O
-
Saccharomyces cerevisiae ?
-
?
angiotensinogen 1-14 + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser, low activity Saccharomyces cerevisiae Asp + Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser
-
?
angiotensinogen 1-14 + H2O
-
Saccharomyces cerevisiae ?
-
?
additional information the enzyme is specific for unblocked N-terminal acidic amino acid residues, no activity with N-acetyl-angiotensinogen 1-14, Tyr-bradykinin, Ile-Ser-bradykinin, and [Sar1, Ala8]-angiotensin II, overview Saccharomyces cerevisiae ?
-
?
additional information the enzyme is specific for unblocked N-terminal acidic amino acid residues, no activity with N-acetyl-angiotensinogen 1-14, Tyr-bradykinin, Ile-Ser-bradykinin, and [Sar1, Ala8]-angiotensin II, overview Saccharomyces cerevisiae B-8032 ?
-
?

Subunits

Subunits Comment Organism
dodecamer 12 * 56000, SDS-PAGE Saccharomyces cerevisiae
dodecamer dodecamer comprising 12 identical units Saccharomyces cerevisiae
More peptide mass fingerprinting by MALDI-TOF MS analysis Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Aminopeptidase
-
Saccharomyces cerevisiae
aspartyl aminopeptidase
-
Saccharomyces cerevisiae
Yhr113w
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Saccharomyces cerevisiae
37
-
activity assay Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
neutral pH-optimum Saccharomyces cerevisiae