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Literature summary for 3.4.11.21 extracted from

  • Simmons, W.H.
    Aspartyl aminopeptidase (2004), Handbook of Proteolytic Enzymes (2nd Edition), 1, 937-939.
No PubMed abstract available
Search for more literature for 3.4.11.21

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Mus musculus
DNA and amino acid sequence determination and analysis, expression in Escherichia coli as His6-tagged enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline complete inhibition Homo sapiens
1,10-phenanthroline complete inhibition Mus musculus
1,10-phenanthroline complete inhibition Oryctolagus cuniculus
DTT high inhibition at 1 mM Homo sapiens
DTT high inhibition at 1 mM Mus musculus
DTT 88% inhibition at 1 mM Oryctolagus cuniculus
EDTA weak inhibition Homo sapiens
EDTA weak inhibition Mus musculus
EDTA weak inhibition Oryctolagus cuniculus
additional information no inhibition by Zn2+ in contrary to glutamyl aminopeptidase, EC 3.4.11.7, no inhibition by Glu-thiol, Asp-thiol, bestatin, amastatin, and puromycin Homo sapiens
additional information no inhibition by Zn2+ in contrary to glutamyl aminopeptidase, EC 3.4.11.7, no inhibition by Glu-thiol, Asp-thiol, bestatin, amastatin, and puromycin Mus musculus
additional information no inhibition by Zn2+ in contrary to glutamyl aminopeptidase, EC 3.4.11.7, no inhibition by Glu-thiol, Asp-thiol, bestatin, amastatin, and puromycin Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Mus musculus 5737
-
cytoplasm
-
 Homo sapiens 5737
-
cytoplasm
-
 Oryctolagus cuniculus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is not affected by chloride ions Mus musculus
additional information the enzyme is not affected by chloride ions Homo sapiens
additional information the enzyme is not affected by chloride ions Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52428
-
 8 * 55000, SDS-PAGE, 8 * 52428, amino acid sequence calculation Homo sapiens
55000
-
 8 * 55000, SDS-PAGE, 8 * 52428, amino acid sequence calculation Homo sapiens
440000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity Homo sapiens
soluble enzyme from brain to homogeneity by affinity chromatography, gel filtration, hydrophobic interaction and hydroxyapatite chromatography Oryctolagus cuniculus

Reaction

Reaction Comment Organism Reaction ID
release of an N-terminal aspartate or glutamate from a peptide, with a preference for aspartate the conserved residues His94, His170, and His440 are essential for activity Mus musculus
a
release of an N-terminal aspartate or glutamate from a peptide, with a preference for aspartate the conserved residues His94, His170, and His440 are essential for activity Homo sapiens
a
release of an N-terminal aspartate or glutamate from a peptide, with a preference for aspartate the conserved residues His94, His170, and His440 are essential for activity Oryctolagus cuniculus
a

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Oryctolagus cuniculus
-
additional information broad tissue distribution Mus musculus
-
additional information broad tissue distribution Homo sapiens
-
additional information broad tissue distribution Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin II + H2O i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe Mus musculus Asp + angiotensin III i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe ?
angiotensin II + H2O i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe Homo sapiens Asp + angiotensin III i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe ?
angiotensin II + H2O i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe Oryctolagus cuniculus Asp + angiotensin III i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe ?
Asp-Ala-Pro-chromogen + H2O
-
 Mus musculus Asp + Ala-Pro-chromogen
-
 ?
Asp-Ala-Pro-chromogen + H2O
-
 Homo sapiens Asp + Ala-Pro-chromogen
-
 ?
Asp-Ala-Pro-chromogen + H2O
-
 Oryctolagus cuniculus Asp + Ala-Pro-chromogen
-
 ?
Asp-Lys-Ala-Leu + H2O lower activity Mus musculus Asp + Lys-Ala-Leu
-
 ?
Asp-Lys-Ala-Leu + H2O lower activity Homo sapiens Asp + Lys-Ala-Leu
-
 ?
Asp-Lys-Ala-Leu + H2O lower activity Oryctolagus cuniculus Asp + Lys-Ala-Leu
-
 ?
additional information substrate specificity, overview, the substrate chain length is important for activity, the enzyme has little or no activity towards aminoacyl-arylamines, no or little activity towards aspartyl dipeptides with a positively charged residue in the second position Mus musculus ?
-
 ?
additional information substrate specificity, overview, the substrate chain length is important for activity, the enzyme has little or no activity towards aminoacyl-arylamines, no or little activity towards aspartyl dipeptides with a positively charged residue in the second position Homo sapiens ?
-
 ?
additional information substrate specificity, overview, the substrate chain length is important for activity, the enzyme has little or no activity towards aminoacyl-arylamines, no or little activity towards aspartyl dipeptides with a positively charged residue in the second position Oryctolagus cuniculus ?
-
 ?
N-(alpha-L-aspartyl)-4-nitroanilide + H2O
-
 Mus musculus L-aspartic acid + 4-nitroaniline
-
 ?
N-(alpha-L-aspartyl)-4-nitroanilide + H2O
-
 Homo sapiens L-aspartic acid + 4-nitroaniline
-
 ?
N-(alpha-L-aspartyl)-4-nitroanilide + H2O
-
 Oryctolagus cuniculus L-aspartic acid + 4-nitroaniline
-
 ?
N-(alpha-L-glutamyl)-2-naphthylamide + H2O
-
 Mus musculus L-glutamic acid + 2-naphthylamine
-
 ?
N-(alpha-L-glutamyl)-2-naphthylamide + H2O
-
 Homo sapiens L-glutamic acid + 2-naphthylamine
-
 ?
N-(alpha-L-glutamyl)-2-naphthylamide + H2O
-
 Oryctolagus cuniculus L-glutamic acid + 2-naphthylamine
-
 ?
N-(alpha-L-glutamyl)-4-nitroanilide + H2O
-
 Mus musculus L-glutamic acid + 4-nitroaniline
-
 ?
N-(alpha-L-glutamyl)-4-nitroanilide + H2O
-
 Homo sapiens L-glutamic acid + 4-nitroaniline
-
 ?
N-(alpha-L-glutamyl)-4-nitroanilide + H2O
-
 Oryctolagus cuniculus L-glutamic acid + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 55000, SDS-PAGE, 8 * 52428, amino acid sequence calculation Homo sapiens

Synonyms

Synonyms Comment Organism
DAP
-
 Mus musculus
DAP
-
 Homo sapiens
DAP
-
 Oryctolagus cuniculus
More the enzyme belongs to the peptidase family M18 Mus musculus
More the enzyme belongs to the peptidase family M18 Homo sapiens
More the enzyme belongs to the peptidase family M18 Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 neutral pH-optimum Mus musculus
additional information
-
 neutral pH-optimum Homo sapiens
additional information
-
 neutral pH-optimum Oryctolagus cuniculus
7.5
-
 assay at Mus musculus
7.5
-
 assay at Homo sapiens
7.5
-
 assay at Oryctolagus cuniculus

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens sequence calculation
-
 7.03