Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Aspergillus oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 57% residual activity at 40 mM; 57% residual activity at 40 mM | Aspergillus oryzae | |
EDTA | 54% residual activity at 20 mM; 54% residual activity at 20 mM | Aspergillus oryzae | |
NaCl | about 30% of activity is retained in 20% (w/v) NaCl; about 30% of activity is retained in 20% (w/v) NaCl | Aspergillus oryzae | |
o-phenanthroline | 43% residual activity at 5 mM; 43% residual activity at 5 mM | Aspergillus oryzae | |
Zn2+ | 27% residual activity at 0.4 mM; 27% residual activity at 0.4 mM | Aspergillus oryzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 294% increase of activity at 0.4 mM | Aspergillus oryzae | |
Mn2+ | 91% increase of activity at 0.4 mM | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54430 | - |
calculated from amino acid sequence | Aspergillus oryzae |
57700 | - |
SDS-PAGE | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | Q2U9Z9 | - |
- |
Aspergillus oryzae | Q2UPZ7 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose 6 Fast Flow column chromatography | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin II + H2O | - |
Aspergillus oryzae | Asp + angiotensin III | - |
? | |
Asp-p-nitroanilide + H2O | 64% activity | Aspergillus oryzae | Asp + p-nitroaniline | - |
? | |
Glu-p-nitroanilide + H2O | 100% activity | Aspergillus oryzae | Glu + p-nitroaniline | - |
? | |
additional information | does not hydrolyze [Asn1, Val5]-angiotensin II, less than 0.5% activity on Ala-p-nitroanilide, Pro-p-nitroanilide, Met-p-nitroanilide, Arg-p-nitroanilide, and Leu-p-nitroanilide | Aspergillus oryzae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
angiotensinase | - |
Aspergillus oryzae |
AspAP | - |
Aspergillus oryzae |
aspartyl-AP | - |
Aspergillus oryzae |
DAP | - |
Aspergillus oryzae |
peptidase E | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme remains stabel upt to 50°C and retains about 70% activity after 20 h at 50°C, is inactivated rapidly at temperature in excess of this | Aspergillus oryzae |
50 | - |
the enzyme remains stable upt to 50°C and retains about 70% activity after 20 h at 50°C, is inactivated rapidly at temperature in excess of this | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Aspergillus oryzae |