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Literature summary for 3.4.11.3 extracted from

  • Matsumoto, H.; Rogi, T.; Yamashiro, K.; Kodama, S.; Tsuruoka, N.; Hattori, A.; Takio, K.; Mizutani, S.; Tsujimoto, M.
    Characterization of a recombinant soluble form of human placental leucine aminopeptidase/oxytocinase expressed in Chinese hamster ovary cells (2000), Eur. J. Biochem., 267, 46-52.
    View publication on PubMed
Search for more literature for 3.4.11.3

Cloned(Commentary)

Cloned (Comment) Organism
leucine aminopeptidase/oxytocinase, expression in Chinese hamster ovary cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 0.1 mM, 83% inhibition. 0.01 mM, 10% inhibition Homo sapiens
amastatin 0.1 mM, 96% inhibition. 0.01 mM, 79% inhibition Homo sapiens
bestatin 0.1 mM, 43% inhibition. 0.01 mM, 12% inhibition Homo sapiens
CuCl2 0.1 mM, 91% inhibition. 0.01 mM, 87% inhibition Homo sapiens
leupeptin 0.1 mM, 53% inhibition Homo sapiens
additional information EDTA has no effect Homo sapiens
pepstatin A 0.1 mM, 70% inhibition Homo sapiens
puromycin 0.1 mM, 36% inhibition. 0.01 mM, 5% inhibition Homo sapiens
ZnCl2 0.1 mM, 98% inhibition. 0.01 mM, 77% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0225
-
 Leu-4-methylcoumaryl-7-amide pH 7.5, 37°C Homo sapiens
0.0436
-
 S-benzyl-Cys-4-methylcoumaryl-7-amide pH 7.5, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound to Homo sapiens 16020
-
soluble recombinant enzyme expressed in Chinese hamster ovary cells Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc enzyme contains 1 mol of zinc per mol of enzyme, contains the consensus HEXXH(X)18E motif of zinc-metallopeptidase proteins Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
 2 * 170000, SDS-PAGE Homo sapiens
280000
-
 sedimentation velocity experiments Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
 Homo sapiens
-
serum serum level of the enzyme increases with gestation Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens Arg + 7-amino-4-methylcoumarin
-
 ?
Leu-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens Leu + 7-amino-4-methylcoumarin
-
 ?
Lys-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens Lys + 7-amino-4-methylcoumarin
-
 ?
Met-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens Met + 7-amino-4-methylcoumarin
-
 ?
S-benzyl-Cys-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens S-benzyl-Cys + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 170000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
leucine aminopeptidase/oxytocinase
-
 Homo sapiens
P-LAP
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04 1.97 Leu-4-methylcoumaryl-7-amide pH 7.5, 37°C Homo sapiens
9.18
-
 Leu-4-methylcoumaryl-7-amide pH 7.5, 37°C Homo sapiens
12.4
-
 S-benzyl-Cys-4-methylcoumaryl-7-amide pH 7.5, 37°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0014
-
 amastatin pH 7.5, 37°C Homo sapiens