Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.11.5 extracted from

  • Bolumar, T.; Sanz, Y.; Aristoy, M.C.; Toldra, F.
    Purification and characterization of a prolyl aminopeptidase from Debaryomyces hansenii (2003), Appl. Environ. Microbiol., 69, 227-232.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.5

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoic acid 61% remaining activity at 1 mM Debaryomyces hansenii
Hg2+
-
 Debaryomyces hansenii
iodoacetate 2.6% remaining activity at 1 mM Debaryomyces hansenii
additional information not affected by bestatin, puromycin, reducing agents, chelating agents and cations Debaryomyces hansenii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 proline-7-amido-4-methylcoumarin pH 7.5, 37°C Debaryomyces hansenii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53500
-
 7 * 53500, SDS-PAGE Debaryomyces hansenii
370000
-
 gel filtration Debaryomyces hansenii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Debaryomyces hansenii enzyme possibly plays a role in meat fermentation ?
-
 ?
additional information Debaryomyces hansenii CECT12487 enzyme possibly plays a role in meat fermentation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Debaryomyces hansenii
-
-
-
Debaryomyces hansenii CECT12487
-
-
-

Purification (Commentary)

Purification (Comment) Organism
248fold Debaryomyces hansenii

Reaction

Reaction Comment Organism Reaction ID
release of N-terminal proline from a peptide the enzyme exclusively hydrolyzes N-terminal-proline-containing substrates Debaryomyces hansenii
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.06
-
 purified enzyme Debaryomyces hansenii

Storage Stability

Storage Stability Organism
4°C, purified enzyme, stable for at least 3 months Debaryomyces hansenii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity, no activity with any other Xaa-7-amido-4-methylcoumarin substrate, no activity with substrates Leu-Pro, Leu-Leu, Val-Val, Gly-Pro, Lys-Lys, Glu-Glu, and Ala-Ala-Ala Debaryomyces hansenii ?
-
 ?
additional information enzyme possibly plays a role in meat fermentation Debaryomyces hansenii ?
-
 ?
additional information substrate specificity, no activity with any other Xaa-7-amido-4-methylcoumarin substrate, no activity with substrates Leu-Pro, Leu-Leu, Val-Val, Gly-Pro, Lys-Lys, Glu-Glu, and Ala-Ala-Ala Debaryomyces hansenii CECT12487 ?
-
 ?
additional information enzyme possibly plays a role in meat fermentation Debaryomyces hansenii CECT12487 ?
-
 ?
Pro-Gly + H2O
-
 Debaryomyces hansenii Pro + Gly
-
 ?
Pro-Gly + H2O
-
 Debaryomyces hansenii CECT12487 Pro + Gly
-
 ?
Pro-Leu + H2O
-
 Debaryomyces hansenii Pro + Leu
-
 ?
Pro-Leu + H2O
-
 Debaryomyces hansenii CECT12487 Pro + Leu
-
 ?
Pro-Phe + H2O
-
 Debaryomyces hansenii Pro + Phe
-
 ?
Pro-Phe + H2O
-
 Debaryomyces hansenii CECT12487 Pro + Phe
-
 ?
Pro-Phe-Gly-Lys + H2O
-
 Debaryomyces hansenii Pro + Phe-Gly-Lys
-
 ?
proline-7-amido-4-methylcoumarin + H2O
-
 Debaryomyces hansenii proline + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
heptamer 7 * 53500, SDS-PAGE Debaryomyces hansenii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Debaryomyces hansenii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Debaryomyces hansenii

pH Range

pH Minimum pH Maximum Comment Organism
5 9.5
-
 Debaryomyces hansenii