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Literature summary for 3.4.11.5 extracted from
- The molecular and biochemical characteristics of proline iminopeptidase from rye seedlings (Secale cereale L.) (2006), Acta Physiol. Plant., 28, 517-524.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuCl2 | 1 mM, 9% residual activity | Secale cereale |  |
| diisopropyl fluorophosphate | 1 mM, 49% residual activity | Secale cereale | |
| E-64 | 1 mM, 16% residual activity | Secale cereale | |
| additional information | not inhibitory: MgCl2, MnCl2, CaCl2 | Secale cereale | |
| p-hydroxymercuribenzoic acid | 1 mM, 2% residual activity | Secale cereale | |
| PbCl2 | 1 mM, 9% residual activity | Secale cereale | |
| phenylmethylsulfonyl fluoride | 1 mM, 51% residual activity | Secale cereale | |
| ZnCl2 | 1 mM, 9% residual activity | Secale cereale | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 225 | - |
gel filtration | Secale cereale |
| 55400 | - |
4 * 55400, SDS-PAGE | Secale cereale |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Secale cereale | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Secale cereale |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | shoot of seedling | Secale cereale | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
pH 7.5, 37°C | Secale cereale |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycyl-2-naphthylamide + H2O | 6.4% of the activity with L-prolyl-2-naphthylamide | Secale cereale | glycine + 2-naphthylamine | - |
? | |
| L-alanyl-2-naphthylamide + H2O | 6.1% of the activity with L-prolyl-2-naphthylamide | Secale cereale | L-alanine + 2-naphthylamine | - |
? | |
| L-arginyl-2-naphthylamide + H2O | 3.2% of the activity with L-prolyl-2-naphthylamide | Secale cereale | L-arginine + 2-naphthylamine | - |
? | |
| L-histidinyl-2-naphthylamide + H2O | 6.3% of the activity with L-prolyl-2-naphthylamide | Secale cereale | L-histidine + 2-naphthylamine | - |
? | |
| L-leucyl-2-naphthylamide + H2O | 4.7% of the activity with L-prolyl-2-naphthylamide | Secale cereale | L-leucine + 2-naphthylamine | - |
? | |
| L-phenylalanyl-2-naphthylamide + H2O | 7.6% of the activity with L-prolyl-2-naphthylamide | Secale cereale | L-phenylalanine + 2-naphthylamine | - |
? | |
| L-prolyl-2-naphthylamide + H2O | - |
Secale cereale | L-proline + 2-naphthylamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 55400, SDS-PAGE | Secale cereale |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Secale cereale |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
30 min, stable up to | Secale cereale |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Secale cereale |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Secale cereale |
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