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Literature summary for 3.4.11.5 extracted from

  • Uraji, M.; Arima, J.; Uesugi, Y.; Iwabuchi, M.; Hatanaka, T.
    Effect of salt on the activity of Streptomyces prolyl aminopeptidase (2007), Biochim. Biophys. Acta, 1774, 1462-1469.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces lividans
expression in Escherichia coli. Enzyme carrying a C-terminal His-tag is less stable than native enzyme Kitasatospora aureofaciens

Protein Variants

Protein Variants Comment Organism
additional information construction of chimera between the salt-sensitive enzyme from Streptomyces lividans and the salt-resistant enzyme from Streptomyces aureofaciens. The fine tuning of the N-terminal conformation of Streptomyces aureofaciens enzyme by hydrophobic interaction is important for the salt tolerance Kitasatospora aureofaciens
additional information construction of chimera between the salt-sensitive enzyme from Streptomyces lividans and the salt-resistant enzyme from Streptomyces aureofaciens. The fine tuning of the N-terminal conformation of Streptomyces aureofaciens enzyme by hydrophobic interaction is important for the salt tolerance Streptomyces lividans

Inhibitors

Inhibitors Comment Organism Structure
additional information enzyme is still active in 4.0 M NaCl Kitasatospora aureofaciens
p-chloromercuribenzoic acid
-
Kitasatospora aureofaciens
pefabloc
-
Kitasatospora aureofaciens
phenylmethylsulfonyl fluoride
-
Kitasatospora aureofaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
6 * 42000, SDS-PAGE, 6 * 47519, calculated Kitasatospora aureofaciens
47519
-
6 * 42000, SDS-PAGE, 6 * 47519, calculated Kitasatospora aureofaciens
250000
-
gel filtration Kitasatospora aureofaciens

Organism

Organism UniProt Comment Textmining
Kitasatospora aureofaciens
-
-
-
Kitasatospora aureofaciens TH-3
-
-
-
Streptomyces lividans
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
Kitasatospora aureofaciens
-
culture fluid
-
Streptomyces lividans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.28
-
pH 7.0, 30°C Kitasatospora aureofaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Pro-4-nitroanilide + H2O
-
Streptomyces lividans L-Pro + 4-nitroaniline
-
?
L-Pro-4-nitroanilide + H2O highly selective Kitasatospora aureofaciens L-Pro + 4-nitroaniline
-
?
L-Pro-4-nitroanilide + H2O highly selective Kitasatospora aureofaciens TH-3 L-Pro + 4-nitroaniline
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 42000, SDS-PAGE, 6 * 47519, calculated Kitasatospora aureofaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Kitasatospora aureofaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
up to Kitasatospora aureofaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.8
-
Kitasatospora aureofaciens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9
-
Kitasatospora aureofaciens