Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces lividans |
expression in Escherichia coli. Enzyme carrying a C-terminal His-tag is less stable than native enzyme | Kitasatospora aureofaciens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of chimera between the salt-sensitive enzyme from Streptomyces lividans and the salt-resistant enzyme from Streptomyces aureofaciens. The fine tuning of the N-terminal conformation of Streptomyces aureofaciens enzyme by hydrophobic interaction is important for the salt tolerance | Kitasatospora aureofaciens |
additional information | construction of chimera between the salt-sensitive enzyme from Streptomyces lividans and the salt-resistant enzyme from Streptomyces aureofaciens. The fine tuning of the N-terminal conformation of Streptomyces aureofaciens enzyme by hydrophobic interaction is important for the salt tolerance | Streptomyces lividans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is still active in 4.0 M NaCl | Kitasatospora aureofaciens | |
p-chloromercuribenzoic acid | - |
Kitasatospora aureofaciens | |
pefabloc | - |
Kitasatospora aureofaciens | |
phenylmethylsulfonyl fluoride | - |
Kitasatospora aureofaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
6 * 42000, SDS-PAGE, 6 * 47519, calculated | Kitasatospora aureofaciens |
47519 | - |
6 * 42000, SDS-PAGE, 6 * 47519, calculated | Kitasatospora aureofaciens |
250000 | - |
gel filtration | Kitasatospora aureofaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kitasatospora aureofaciens | - |
- |
- |
Kitasatospora aureofaciens TH-3 | - |
- |
- |
Streptomyces lividans | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture fluid | - |
Kitasatospora aureofaciens | - |
culture fluid | - |
Streptomyces lividans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.28 | - |
pH 7.0, 30°C | Kitasatospora aureofaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Pro-4-nitroanilide + H2O | - |
Streptomyces lividans | L-Pro + 4-nitroaniline | - |
? | |
L-Pro-4-nitroanilide + H2O | highly selective | Kitasatospora aureofaciens | L-Pro + 4-nitroaniline | - |
? | |
L-Pro-4-nitroanilide + H2O | highly selective | Kitasatospora aureofaciens TH-3 | L-Pro + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 42000, SDS-PAGE, 6 * 47519, calculated | Kitasatospora aureofaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Kitasatospora aureofaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
up to | Kitasatospora aureofaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.8 | - |
Kitasatospora aureofaciens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Kitasatospora aureofaciens |