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Literature summary for 3.4.11.5 extracted from
- Extraction, purification and characterization of low molecular weight proline iminopeptidase from probiotic L. plantarum for meat tenderization (2018), Int. J. Biol. Macromol., 109, 651-663 .
Application
| Application | Comment | Organism |
|---|---|---|
| industry | the effectiveness of enzyme in purified form, membrane bound form and in combination with other enzymes to degrade collagen resulting in pharmaceutically significant collagen hydrolysates and in meat tenderization marks its industrial importance | Lactiplantibacillus plantarum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-(2-aminoethyl)benzenesulfonyl fluoride | 85% inhibition at 1 mM | Lactiplantibacillus plantarum |  |
| 4-chloromercuribenzoate | 40% inhibition at 1 mM | Lactiplantibacillus plantarum | |
| 4-nitrophenyl iodoacetate | 56% inhibition at 1 mM | Lactiplantibacillus plantarum | |
| cysteine | 26% inhibition at 1 mM | Lactiplantibacillus plantarum | |
| DTT | 14% inhibition at 1 mM | Lactiplantibacillus plantarum | |
| EDTA | complete inhibition at 1 mM | Lactiplantibacillus plantarum | |
| glutathione | 86% inhibition at 1 mM | Lactiplantibacillus plantarum | |
| additional information | no inhibition by PMSF, 2-mercaptoethanol, and diethyl dicarbonate | Lactiplantibacillus plantarum | |
| N-ethylmaleimide | 72% inhibition at 1 mM | Lactiplantibacillus plantarum | |
| puromycin | 54% inhibition at 1 mM | Lactiplantibacillus plantarum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Lactiplantibacillus plantarum | |
| 0.065 | - |
Pro-4-methoxy-beta-naphythylamide | pH 7.4, 37°C | Lactiplantibacillus plantarum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane bound | Lactiplantibacillus plantarum | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | metal ions and EDTA show no effect on enzyme activity | Lactiplantibacillus plantarum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37900 | - |
gel filtration | Lactiplantibacillus plantarum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lacticaseibacillus paracasei subsp. paracasei | C2F9L5 | - |
- |
| Lacticaseibacillus paracasei subsp. paracasei ATCC 25302 | C2F9L5 | - |
- |
| Lacticaseibacillus paracasei subsp. paracasei NCDC 384 | C2F9L5 | - |
- |
| Lacticaseibacillus rhamnosus | C7TMK0 | - |
- |
| Lacticaseibacillus rhamnosus LC 705 | C7TMK0 | - |
- |
| Lacticaseibacillus rhamnosus NCDC 347 | C7TMK0 | - |
- |
| Lactiplantibacillus plantarum | Q890D8 | - |
- |
| Lactiplantibacillus plantarum ATCC BAA-793 | Q890D8 | - |
- |
| Lactiplantibacillus plantarum NCDC 020 | Q890D8 | - |
- |
| Lactiplantibacillus plantarum NCIMB 8826 | Q890D8 | - |
- |
| Lactobacillus acidophilus | Q5FMT1 | - |
- |
| Lactobacillus acidophilus ATCC 700396 | Q5FMT1 | - |
- |
| Lactobacillus acidophilus N2 | Q5FMT1 | - |
- |
| Lactobacillus acidophilus NCDC 013 | Q5FMT1 | - |
- |
| Lactobacillus acidophilus NCFM | Q5FMT1 | - |
- |
| Lactobacillus acidophilus NCK56 | Q5FMT1 | - |
- |
| Levilactobacillus brevis | Q03NE0 | - |
- |
| Levilactobacillus brevis ATCC 367 | Q03NE0 | - |
- |
| Levilactobacillus brevis JCM 1170 | Q03NE0 | - |
- |
| Levilactobacillus brevis NCDC 371 | Q03NE0 | - |
- |
| Limosilactobacillus fermentum | C0WZ89 | - |
- |
| Limosilactobacillus fermentum ATCC 14931 | C0WZ89 | - |
- |
| Limosilactobacillus fermentum NCDC 406 | C0WZ89 | - |
- |
| Loigolactobacillus coryniformis | - |
- |
- |
| Loigolactobacillus coryniformis NCDC 366 | - |
- |
- |
| Pediococcus acidilactici | A0A1A5VII5 | i.e. Lactobacillus acidilactici NCDC 252 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| membrane bound enzyme 7.13fold by cation exchange chromatography, gel filtration, and anion exchange chromatography | Lactiplantibacillus plantarum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26.4 | - |
purified native enzyme, pH 7.4, 37°C | Lactiplantibacillus plantarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is a serine protease | Loigolactobacillus coryniformis | ? | - |
? | |
| additional information | the enzyme is a serine protease | Pediococcus acidilactici | ? | - |
? | |
| additional information | the enzyme is a serine protease | Lacticaseibacillus rhamnosus | ? | - |
? | |
| additional information | the enzyme is a serine protease | Lactobacillus acidophilus | ? | - |
? | |
| additional information | the enzyme is a serine protease | Lactiplantibacillus plantarum | ? | - |
? | |
| additional information | the enzyme is a serine protease | Lacticaseibacillus paracasei subsp. paracasei | ? | - |
? | |
| additional information | the enzyme is a serine protease | Levilactobacillus brevis | ? | - |
? | |
| additional information | the enzyme is a serine protease | Limosilactobacillus fermentum | ? | - |
? | |
| additional information | the enzyme is a serine protease | Levilactobacillus brevis ATCC 367 | ? | - |
? | |
| additional information | the enzyme is a serine protease | Lactiplantibacillus plantarum NCIMB 8826 | ? | - |
? | |
| additional information | the enzyme is a serine protease | Lactobacillus acidophilus NCDC 013 | ? | - |
? | |
| additional information | the enzyme is a serine protease | Lacticaseibacillus paracasei subsp. paracasei NCDC 384 | ? | - |
? | |
| additional information | the enzyme is a serine protease | Lacticaseibacillus rhamnosus NCDC 347 | ? | - |
? | |
| additional information | the enzyme is a serine protease | Lactiplantibacillus plantarum ATCC BAA-793 | ? | - |
? | |
| additional information | the enzyme is a serine protease | Levilactobacillus brevis NCDC 371 | ? | - |
? | |
| additional information | the enzyme is a serine protease | Lacticaseibacillus paracasei subsp. paracasei ATCC 25302 | ? | - |
? | |
| additional information | the enzyme is a serine protease | Lacticaseibacillus rhamnosus LC 705 | ? | - |
? | |
| additional information | the enzyme is a serine protease | Lactobacillus acidophilus NCFM | ? | - |
? | |
| additional information | the enzyme is a serine protease | Loigolactobacillus coryniformis NCDC 366 | ? | - |
? | |
| additional information | the enzyme is a serine protease | Lactobacillus acidophilus ATCC 700396 | ? | - |
? | |
| additional information | the enzyme is a serine protease | Levilactobacillus brevis JCM 1170 | ? | - |
? | |
| additional information | the enzyme is a serine protease | Limosilactobacillus fermentum NCDC 406 | ? | - |
? | |
| additional information | the enzyme is a serine protease | Limosilactobacillus fermentum ATCC 14931 | ? | - |
? | |
| additional information | the enzyme is a serine protease | Lactobacillus acidophilus NCK56 | ? | - |
? | |
| additional information | the enzyme is a serine protease | Lactobacillus acidophilus N2 | ? | - |
? | |
| additional information | the enzyme is a serine protease | Lactiplantibacillus plantarum NCDC 020 | ? | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Loigolactobacillus coryniformis | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Pediococcus acidilactici | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Lacticaseibacillus rhamnosus | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Lactobacillus acidophilus | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Lactiplantibacillus plantarum | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Lacticaseibacillus paracasei subsp. paracasei | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Levilactobacillus brevis | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Limosilactobacillus fermentum | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Levilactobacillus brevis ATCC 367 | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Lactiplantibacillus plantarum NCIMB 8826 | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Lactobacillus acidophilus NCDC 013 | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Lacticaseibacillus paracasei subsp. paracasei NCDC 384 | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Lacticaseibacillus rhamnosus NCDC 347 | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Lactiplantibacillus plantarum ATCC BAA-793 | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Levilactobacillus brevis NCDC 371 | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Lacticaseibacillus paracasei subsp. paracasei ATCC 25302 | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Lacticaseibacillus rhamnosus LC 705 | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Lactobacillus acidophilus NCFM | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Loigolactobacillus coryniformis NCDC 366 | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Lactobacillus acidophilus ATCC 700396 | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Levilactobacillus brevis JCM 1170 | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Limosilactobacillus fermentum NCDC 406 | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Limosilactobacillus fermentum ATCC 14931 | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Lactobacillus acidophilus NCK56 | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Lactobacillus acidophilus N2 | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
| Pro-4-methoxy-beta-naphythylamide + H2O | - |
Lactiplantibacillus plantarum NCDC 020 | Pro + 4-methoxy-beta-naphythylamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 18900, SDS-PAGE | Lactiplantibacillus plantarum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PIP | - |
Loigolactobacillus coryniformis |
| PIP | - |
Pediococcus acidilactici |
| PIP | - |
Lacticaseibacillus rhamnosus |
| PIP | - |
Lactobacillus acidophilus |
| PIP | - |
Lactiplantibacillus plantarum |
| PIP | - |
Lacticaseibacillus paracasei subsp. paracasei |
| PIP | - |
Levilactobacillus brevis |
| PIP | - |
Limosilactobacillus fermentum |
| proline iminopeptidase | - |
Loigolactobacillus coryniformis |
| proline iminopeptidase | - |
Pediococcus acidilactici |
| proline iminopeptidase | - |
Lacticaseibacillus rhamnosus |
| proline iminopeptidase | - |
Lactobacillus acidophilus |
| proline iminopeptidase | - |
Lactiplantibacillus plantarum |
| proline iminopeptidase | - |
Lacticaseibacillus paracasei subsp. paracasei |
| proline iminopeptidase | - |
Levilactobacillus brevis |
| proline iminopeptidase | - |
Limosilactobacillus fermentum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Loigolactobacillus coryniformis |
| 37 | - |
assay at | Pediococcus acidilactici |
| 37 | - |
assay at | Lacticaseibacillus rhamnosus |
| 37 | - |
assay at | Lactobacillus acidophilus |
| 37 | - |
assay at | Lacticaseibacillus paracasei subsp. paracasei |
| 37 | - |
assay at | Levilactobacillus brevis |
| 37 | - |
assay at | Limosilactobacillus fermentum |
| 45 | - |
- |
Lactiplantibacillus plantarum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 60 | activity range, profile overview | Lactiplantibacillus plantarum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
70 | purified native enzyme, completely stable up to 45°C, loss of 30% activity at 55°C, loss of over 80% activity at 60°C, and inactivation at 70°C | Lactiplantibacillus plantarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Lactiplantibacillus plantarum |
| 7.4 | - |
assay at | Loigolactobacillus coryniformis |
| 7.4 | - |
assay at | Pediococcus acidilactici |
| 7.4 | - |
assay at | Lactobacillus acidophilus |
| 7.4 | - |
assay at | Lacticaseibacillus paracasei subsp. paracasei |
| 7.4 | - |
assay at | Levilactobacillus brevis |
| 7.4 | - |
assay at | Limosilactobacillus fermentum |
| 7.4 | - |
asssay at | Lacticaseibacillus rhamnosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | activity range, profile overview | Lactiplantibacillus plantarum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | purified native enzyme, about, over 80% activity remaining | Lactiplantibacillus plantarum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the enzyme from Lactobacillus acidophilus shows very low activity compared to the Lactobacillus plantarum strain NCDC 020 enzyme | Lactobacillus acidophilus |
| additional information | the enzyme from Lactobacillus brevis shows about one third of the activity of the Lactobacillus plantarum strain NCDC 020 enzyme | Levilactobacillus brevis |
| additional information | the enzyme from Lactobacillus corniformis shows about half of the activity of Lactobacillus plantarum strain NCDC 020 enzyme | Loigolactobacillus coryniformis |
| additional information | the enzyme from Lactobacillus fermentum shows very low activity compared to the Lactobacillus plantarum strain NCDC 020 enzyme | Limosilactobacillus fermentum |
| additional information | the enzyme from Lactobacillus paracasei shows about one third of the activity of the Lactobacillus plantarum strain NCDC 020 enzyme | Lacticaseibacillus paracasei subsp. paracasei |
| additional information | the enzyme from Lactobacillus rhamnosus shows almost equally high activity compare to the enzyme from Lactobacillus plantarum strain NCDC 020 | Lacticaseibacillus rhamnosus |
| additional information | the enzyme from Pediococcus acidilactici shows very low activity compared to the Lactobacillus plantarum strain NCDC 020 enzyme | Pediococcus acidilactici |
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