Cloned (Comment) | Organism |
---|---|
- |
Mus musculus |
expressed in CHO cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
N353A | the ratio of turnover number to Km-value is 7% of the wild-type value with alpha-L-Glu-2-naphthylamide as substrate, the ratio of turnover number to Km-value is 3% of the wild-type value with angiotensin II as substrate. The Ki-value for glutamate-thiol is 9.3fold higher than the wild-type value, the Ki-value for glutamine-thiol is 4.3fold higher than the wild-type value, the Ki-value for L-glutamate phosphonic acid is 6.6fold higher than the wild-type value, the Ki-value for D-glutamate phosphonic acid is 3.4fold higher than the wild-type value, the Ki-value for captopril is 86% of the wild-type value. Mutant enzyme is similarly routed and glycosylated to the wild-type enzyme | Mus musculus |
N353D | mutant enzyme exhibits weak and unstable activity, mutant enzyme is trapped intracellularly and partially glycosylated | Mus musculus |
N353Q | the ratio of turnover number to Km-value is 4% of the wild-type value with alpha-L-Glu-2-naphthylamide as substrate, the ratio of turnover number to Km-value is 7% of the wild-type value with alpha-L-Asp-2-naphthylamide as substrate, the ratio of turnover number to Km-value is 5% of the wild-type value with angiotensin II as substrate.The Ki-value for glutamate-thiol is 6.3fold higher than the wild-type value, the Ki-value for glutamine-thiol is 4.4fold higher than the wild-type value, the Ki-value for L-glutamate phosphonic acid is 3.57fold higher than the wild-type value, the Ki-value for D-glutamate phosphonic acid is 1.5fold higher than the wild-type value, the Ki-value for captopril is 2fold higher than the wild-type value. Mutant enzyme is similarly routed and glycosylated to the wild-type enzyme | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
captopril | - |
Mus musculus | |
D-glutamate phosphonic acid | - |
Mus musculus | |
glutamate-thiol | - |
Mus musculus | |
glutamine-thiol | - |
Mus musculus | |
L-glutamate phosphonic acid | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.062 | - |
angiotensin II | pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A | Mus musculus | |
0.073 | - |
angiotensin II | pH 7.4, 37°C, wild-type enzyme | Mus musculus | |
0.161 | - |
angiotensin II | pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q | Mus musculus | |
0.243 | - |
alpha-L-Glu-2-naphthylamide | pH 7.4, 37°C, wild-type enzyme | Mus musculus | |
0.515 | - |
alpha-L-Asp-2-naphthylamide | pH 7.4, 37°C, wild-type enzyme | Mus musculus | |
1.943 | - |
alpha-L-Glu-2-naphthylamide | pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A | Mus musculus | |
2.272 | - |
alpha-L-Asp-2-naphthylamide | pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q | Mus musculus | |
2.307 | - |
alpha-L-Asp-2-naphthylamide | pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A | Mus musculus | |
2.687 | - |
alpha-L-Glu-2-naphthylamide | pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | in CHO cells, mutant enzyme N353D | Mus musculus | 5622 | - |
plasma membrane | of CHO cells, wild-type and mutant enzymes N353Q and N353A exhibit membrane expression | Mus musculus | 5886 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P16406 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | wild-type enzyme and the mutant enzymes N353Q and N353A are equally glycosylated, mutant enzyme N353S is partially glycosylated | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-L-Asp-2-naphthylamide + H2O | - |
Mus musculus | L-Asp + 2-naphthylamine | - |
? | |
alpha-L-Glu-2-naphthylamide + H2O | - |
Mus musculus | alpha-L-Glu + 2-naphthylamine | - |
? | |
angiotensin II + H2O | - |
Mus musculus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
angiotensin II | pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q | Mus musculus | |
4.8 | - |
angiotensin II | pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A | Mus musculus | |
14 | - |
alpha-L-Asp-2-naphthylamide | pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A | Mus musculus | |
20 | - |
angiotensin II | pH 7.4, 37°C, wild-type enzyme | Mus musculus | |
25 | - |
alpha-L-Asp-2-naphthylamide | pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q | Mus musculus | |
102 | - |
alpha-L-Asp-2-naphthylamide | pH 7.4, 37°C, wild-type enzyme | Mus musculus | |
184 | - |
alpha-L-Glu-2-naphthylamide | pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q | Mus musculus | |
227 | - |
alpha-L-Glu-2-naphthylamide | pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A | Mus musculus | |
404 | - |
alpha-L-Glu-2-naphthylamide | pH 7.4, 37°C, wild-type enzyme | Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00006 | - |
L-glutamate phosphonic acid | pH 7.4, 37°C, wild-type enzyme | Mus musculus | |
0.000214 | - |
L-glutamate phosphonic acid | pH 7.4, 37°C, mutant enzyme N363Q | Mus musculus | |
0.000216 | - |
glutamate-thiol | pH 7.4, 37°C, wild-type enzyme | Mus musculus | |
0.000395 | - |
L-glutamate phosphonic acid | pH 7.4, 37°C, mutant enzyme N363A | Mus musculus | |
0.000462 | - |
glutamine-thiol | pH 7.4, 37°C, wild-type enzyme | Mus musculus | |
0.00137 | - |
glutamate-thiol | pH 7.4, 37°C, mutant enzyme N363Q | Mus musculus | |
0.00199 | - |
glutamine-thiol | pH 7.4, 37°C, mutant enzyme N363A | Mus musculus | |
0.002 | - |
glutamate-thiol | pH 7.4, 37°C, mutant enzyme N363A | Mus musculus | |
0.00202 | - |
glutamine-thiol | pH 7.4, 37°C, mutant enzyme N363Q | Mus musculus | |
0.00331 | - |
D-glutamate phosphonic acid | pH 7.4, 37°C, wild-type enzyme | Mus musculus | |
0.00489 | - |
D-glutamate phosphonic acid | pH 7.4, 37°C, mutant enzyme N363Q | Mus musculus | |
0.0112 | - |
D-glutamate phosphonic acid | pH 7.4, 37°C, mutant enzyme N363A | Mus musculus | |
0.0141 | - |
captopril | pH 7.4, 37°C, mutant enzyme N363Q | Mus musculus | |
0.0248 | - |
captopril | pH 7.4, 37°C, mutant enzyme N363A | Mus musculus | |
0.0289 | - |
captopril | pH 7.4, 37°C, wild-type enzyme | Mus musculus |