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Literature summary for 3.4.11.7 extracted from

  • Iturrioz, X.; Rozenfeld, R.; Michaud, A.; Corvol, P.; Llorens-Cortes, C.
    Study of asparagine 353 in aminopeptidase A: characterization of a novel motif (GXMEN) implicated in exopeptidase specificity of monozinc aminopeptidases (2001), Biochemistry, 40, 14440-14448.
    View publication on PubMed
Search for more literature for 3.4.11.7

Cloned(Commentary)

Cloned (Comment) Organism
-
 Mus musculus
expressed in CHO cells Mus musculus

Protein Variants

Protein Variants Comment Organism
N353A the ratio of turnover number to Km-value is 7% of the wild-type value with alpha-L-Glu-2-naphthylamide as substrate, the ratio of turnover number to Km-value is 3% of the wild-type value with angiotensin II as substrate. The Ki-value for glutamate-thiol is 9.3fold higher than the wild-type value, the Ki-value for glutamine-thiol is 4.3fold higher than the wild-type value, the Ki-value for L-glutamate phosphonic acid is 6.6fold higher than the wild-type value, the Ki-value for D-glutamate phosphonic acid is 3.4fold higher than the wild-type value, the Ki-value for captopril is 86% of the wild-type value. Mutant enzyme is similarly routed and glycosylated to the wild-type enzyme Mus musculus
N353D mutant enzyme exhibits weak and unstable activity, mutant enzyme is trapped intracellularly and partially glycosylated Mus musculus
N353Q the ratio of turnover number to Km-value is 4% of the wild-type value with alpha-L-Glu-2-naphthylamide as substrate, the ratio of turnover number to Km-value is 7% of the wild-type value with alpha-L-Asp-2-naphthylamide as substrate, the ratio of turnover number to Km-value is 5% of the wild-type value with angiotensin II as substrate.The Ki-value for glutamate-thiol is 6.3fold higher than the wild-type value, the Ki-value for glutamine-thiol is 4.4fold higher than the wild-type value, the Ki-value for L-glutamate phosphonic acid is 3.57fold higher than the wild-type value, the Ki-value for D-glutamate phosphonic acid is 1.5fold higher than the wild-type value, the Ki-value for captopril is 2fold higher than the wild-type value. Mutant enzyme is similarly routed and glycosylated to the wild-type enzyme Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
captopril
-
 Mus musculus
D-glutamate phosphonic acid
-
 Mus musculus
glutamate-thiol
-
 Mus musculus
glutamine-thiol
-
 Mus musculus
L-glutamate phosphonic acid
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.062
-
 angiotensin II pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A Mus musculus
0.073
-
 angiotensin II pH 7.4, 37°C, wild-type enzyme Mus musculus
0.161
-
 angiotensin II pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q Mus musculus
0.243
-
 alpha-L-Glu-2-naphthylamide pH 7.4, 37°C, wild-type enzyme Mus musculus
0.515
-
 alpha-L-Asp-2-naphthylamide pH 7.4, 37°C, wild-type enzyme Mus musculus
1.943
-
 alpha-L-Glu-2-naphthylamide pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A Mus musculus
2.272
-
 alpha-L-Asp-2-naphthylamide pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q Mus musculus
2.307
-
 alpha-L-Asp-2-naphthylamide pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A Mus musculus
2.687
-
 alpha-L-Glu-2-naphthylamide pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular in CHO cells, mutant enzyme N353D Mus musculus 5622
-
plasma membrane of CHO cells, wild-type and mutant enzymes N353Q and N353A exhibit membrane expression Mus musculus 5886
-

Organism

Organism UniProt Comment Textmining
Mus musculus P16406
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein wild-type enzyme and the mutant enzymes N353Q and N353A are equally glycosylated, mutant enzyme N353S is partially glycosylated Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-L-Asp-2-naphthylamide + H2O
-
 Mus musculus L-Asp + 2-naphthylamine
-
 ?
alpha-L-Glu-2-naphthylamide + H2O
-
 Mus musculus alpha-L-Glu + 2-naphthylamine
-
 ?
angiotensin II + H2O
-
 Mus musculus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4
-
 angiotensin II pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q Mus musculus
4.8
-
 angiotensin II pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A Mus musculus
14
-
 alpha-L-Asp-2-naphthylamide pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A Mus musculus
20
-
 angiotensin II pH 7.4, 37°C, wild-type enzyme Mus musculus
25
-
 alpha-L-Asp-2-naphthylamide pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q Mus musculus
102
-
 alpha-L-Asp-2-naphthylamide pH 7.4, 37°C, wild-type enzyme Mus musculus
184
-
 alpha-L-Glu-2-naphthylamide pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q Mus musculus
227
-
 alpha-L-Glu-2-naphthylamide pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A Mus musculus
404
-
 alpha-L-Glu-2-naphthylamide pH 7.4, 37°C, wild-type enzyme Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00006
-
 L-glutamate phosphonic acid pH 7.4, 37°C, wild-type enzyme Mus musculus
0.000214
-
 L-glutamate phosphonic acid pH 7.4, 37°C, mutant enzyme N363Q Mus musculus
0.000216
-
 glutamate-thiol pH 7.4, 37°C, wild-type enzyme Mus musculus
0.000395
-
 L-glutamate phosphonic acid pH 7.4, 37°C, mutant enzyme N363A Mus musculus
0.000462
-
 glutamine-thiol pH 7.4, 37°C, wild-type enzyme Mus musculus
0.00137
-
 glutamate-thiol pH 7.4, 37°C, mutant enzyme N363Q Mus musculus
0.00199
-
 glutamine-thiol pH 7.4, 37°C, mutant enzyme N363A Mus musculus
0.002
-
 glutamate-thiol pH 7.4, 37°C, mutant enzyme N363A Mus musculus
0.00202
-
 glutamine-thiol pH 7.4, 37°C, mutant enzyme N363Q Mus musculus
0.00331
-
 D-glutamate phosphonic acid pH 7.4, 37°C, wild-type enzyme Mus musculus
0.00489
-
 D-glutamate phosphonic acid pH 7.4, 37°C, mutant enzyme N363Q Mus musculus
0.0112
-
 D-glutamate phosphonic acid pH 7.4, 37°C, mutant enzyme N363A Mus musculus
0.0141
-
 captopril pH 7.4, 37°C, mutant enzyme N363Q Mus musculus
0.0248
-
 captopril pH 7.4, 37°C, mutant enzyme N363A Mus musculus
0.0289
-
 captopril pH 7.4, 37°C, wild-type enzyme Mus musculus