Cloned (Comment) | Organism |
---|---|
expressed in CHO-K1 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | substitution mutations at Asp218 and Asp213 maintain the ability of APA to be activated by Ca2+ and induce a decrease of the inhibitory potencies of inhibitors homologous with acidic residues | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutamate phosphonate | specific aminopeptidase A inhibitor | Mus musculus | |
N-(2-aminoethyl)-4-methyl-5-sulfanyl-pentanamide | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.152 | - |
L-Glu-p-nitroanilide | wild type enzyme, in absence of Ca2+, in 50 mM Tris/HCl buffer (pH 7.4), at 37°C | Mus musculus | |
1.475 | - |
L-Glu-p-nitroanilide | wild type enzyme, in absence of Ca2+, in 50 mM Tris/HCl buffer (pH 7.4), at 37°C | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mus musculus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | each APA monomer contains one Ca2+ atom, Ca2+ (from 0 to 4 mM) increases the activity of the wild type enzyme | Mus musculus | |
Zn2+ | contains zinc | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
minor immature form, SDS-PAGE | Mus musculus |
160000 | - |
major mature form, SDS-PAGE | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P16406 | - |
- |
Purification (Comment) | Organism |
---|---|
Talon Co2+ column chromatography | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin II + H2O | - |
Mus musculus | aspartic acid + angiotensin III | - |
? | |
L-Glu-p-nitroanilide + H2O | - |
Mus musculus | L-Glu + p-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase A | - |
Mus musculus |
APA | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
295 | - |
L-Glu-p-nitroanilide | wild type enzyme, in absence of Ca2+, in 50 mM Tris/HCl buffer (pH 7.4), at 37°C | Mus musculus | |
720 | - |
L-Glu-p-nitroanilide | wild type enzyme, in absence of Ca2+, in 50 mM Tris/HCl buffer (pH 7.4), at 37°C | Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00007 | - |
N-(2-aminoethyl)-4-methyl-5-sulfanyl-pentanamide | wild type enzyme, in 50 mM Tris/HCl buffer (pH 7.4), 4 mM Ca2+, at 37°C | Mus musculus |