Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Rattus norvegicus | |
4-hydroxymercuribenzoate | - |
Rattus norvegicus | |
amastatin | - |
Rattus norvegicus | |
Aprotinin | - |
Rattus norvegicus | |
bestatin | - |
Rattus norvegicus | |
Ca2+ | 1 mM CaCl2, 27% inhibition | Rattus norvegicus | |
Cd2+ | - |
Rattus norvegicus | |
Co2+ | 1 mM CoCl2, 90% inhibition | Rattus norvegicus | |
EDTA | - |
Rattus norvegicus | |
EGTA | - |
Rattus norvegicus | |
Hg2+ | - |
Rattus norvegicus | |
iodoacetate | - |
Rattus norvegicus | |
NEM | - |
Rattus norvegicus | |
Pb2+ | - |
Rattus norvegicus | |
puromycin | - |
Rattus norvegicus | |
Zn2+ | 1 mM ZnSO4, 96% inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
Arg-Pro-Pro | - |
Rattus norvegicus | |
0.28 | - |
bradykinin | - |
Rattus norvegicus | |
0.6 | - |
Tyr-Pro-Phe-Pro-Gly-Pro-Ile | - |
Rattus norvegicus | |
0.72 | - |
Tyr-Pro-Leu-Gly-NH2 | - |
Rattus norvegicus | |
1.02 | - |
Tyr-Pro-Phe-Pro-Gly | - |
Rattus norvegicus | |
1.23 | - |
Arg-Pro | - |
Rattus norvegicus | |
1.33 | - |
L-Tyr-L-Pro-L-Phe-NH2 | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
143000 | - |
with 0.14 M NaCl, gel filtration | Rattus norvegicus |
218000 | - |
without NaCl, gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31.7 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
4°C, bicine buffer, pH 7.8, 1 mM dithiothreitol, 1 mM MnCl2, stable for several weeks | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Pro + H2O | - |
Rattus norvegicus | Ala + Pro | - |
? | |
Arg-Pro + H2O | - |
Rattus norvegicus | Arg + Pro | - |
? | |
Arg-Pro-Pro + H2O | - |
Rattus norvegicus | Arg + Pro-Pro | - |
? | |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O | - |
Rattus norvegicus | Arg + Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg | - |
? | |
L-Tyr-L-Pro-L-Phe-NH2 + H2O | - |
Rattus norvegicus | L-Tyr + L-Pro-L-Phe-NH2 | - |
? | |
additional information | the enzyme liberates all unblocked preferentially basic or hydrophobic ultimate amino acids from dipeptides, tripeptides and oligopeptides with N-terminal Xaa-Pro- sequences, overview | Rattus norvegicus | ? | - |
? | |
Tyr-Pro-Leu-Gly-NH2 + H2O | - |
Rattus norvegicus | ? | - |
? | |
Tyr-Pro-Phe-Pro-Gly + H2O | - |
Rattus norvegicus | ? | - |
? | |
Tyr-Pro-Phe-Pro-Gly-Pro-Ile + H2O | - |
Rattus norvegicus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
pH 7.8, graduall loss of activity | Rattus norvegicus |
45 | - |
pH 7.8, rapid denaturation above | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26 | - |
Tyr-Pro-Phe-Pro-Gly-Pro-Ile | - |
Rattus norvegicus | |
32 | - |
Tyr-Pro-Phe-Pro-Gly | - |
Rattus norvegicus | |
38 | - |
bradykinin | - |
Rattus norvegicus | |
40 | - |
Tyr-Pro-Leu-Gly-NH2 | - |
Rattus norvegicus | |
49 | - |
Arg-Pro-Pro | - |
Rattus norvegicus | |
58 | - |
L-Tyr-L-Pro-L-Phe-NH2 | - |
Rattus norvegicus | |
73 | - |
Arg-Pro | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | 8 | hydrolysis of Arg-Pro-Pro, Ala-Pro or Tyr-Pro-Leu-Gly-NH2 | Rattus norvegicus |