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Literature summary for 3.4.11.9 extracted from

  • Laurent, V.; Brooks, D.R.; Coates, D.; Isaac, R.E.
    Functional expression and characterization of the cytoplasmic aminopeptidase P of Caenorhabditis elegans (2001), Eur. J. Biochem., 268, 5430-5438.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning, DNA and amino acid sequence determination and analysis, and functional expression of open reading frame W03H9.4 from EST yk91g4 in Caenorhabditis elegans as GFP-fusion protein and as His-tagged enzyme in Escherichia coli Caenorhabditis elegans

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Caenorhabditis elegans
apstatin
-
Caenorhabditis elegans
Co2+ stimulatory at 0.01 mM, inhibitory above 0.1 mM Caenorhabditis elegans
EDTA
-
Caenorhabditis elegans
Mn2+
-
Caenorhabditis elegans
Zn2+
-
Caenorhabditis elegans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
bradykinin pH 8.0, 35°C Caenorhabditis elegans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Caenorhabditis elegans 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ stimulatory at 0.01 mM, inhibitory above 0.1 mM Caenorhabditis elegans
additional information metalloenzyme, enzyme which is free of metals due to EDTA-treatment cannot be reactivated by addition of Co2+, Zn2+, or Mn2+ Caenorhabditis elegans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
x * 71000, recombinant His-tagged fusion protein, SDS-PAGE, x * 68000, W03G9.4 protein, SDS-PAGE Caenorhabditis elegans
71000
-
x * 71000, recombinant His-tagged fusion protein, SDS-PAGE, x * 68000, W03G9.4 protein, SDS-PAGE Caenorhabditis elegans

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli Caenorhabditis elegans

Source Tissue

Source Tissue Comment Organism Textmining
intestine expression of GFP-fusion protein Caenorhabditis elegans
-
pharynx expression of GFP-fusion protein Caenorhabditis elegans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bradykinin + H2O i.e. RPPGFSPFR, hydrolysis of the N-terminal Arg1-Pro2 bond Caenorhabditis elegans Arg + des-Arg-bradykinin i.e. PPGFSPFR ?
KPSFVRFamide + H2O a neuropeptide Caenorhabditis elegans Lys + PSFVRFamide
-
?
LemTRP-1 + H2O i.e. APSGFLGVRamide Caenorhabditis elegans Ala + PSGFLGVRamide
-
?
additional information no activity with angiotensin I, des-Arg-bradykinin, AF1, i.e. KNEFIRNRVYIHPFHL, and substance P Caenorhabditis elegans ?
-
?

Subunits

Subunits Comment Organism
? x * 71000, recombinant His-tagged fusion protein, SDS-PAGE, x * 68000, W03G9.4 protein, SDS-PAGE Caenorhabditis elegans

Synonyms

Synonyms Comment Organism
aminopeptidase P
-
Caenorhabditis elegans
AP-P
-
Caenorhabditis elegans
More the enzyme belongs to the peptidase family M24 Caenorhabditis elegans
X-Pro aminopeptidase
-
Caenorhabditis elegans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
assay at Caenorhabditis elegans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 with substrate bradykinin Caenorhabditis elegans