Cloned (Comment) | Organism |
---|---|
cloning, DNA and amino acid sequence determination and analysis, and functional expression of open reading frame W03H9.4 from EST yk91g4 in Caenorhabditis elegans as GFP-fusion protein and as His-tagged enzyme in Escherichia coli | Caenorhabditis elegans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Caenorhabditis elegans | |
apstatin | - |
Caenorhabditis elegans | |
Co2+ | stimulatory at 0.01 mM, inhibitory above 0.1 mM | Caenorhabditis elegans | |
EDTA | - |
Caenorhabditis elegans | |
Mn2+ | - |
Caenorhabditis elegans | |
Zn2+ | - |
Caenorhabditis elegans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
bradykinin | pH 8.0, 35°C | Caenorhabditis elegans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Caenorhabditis elegans | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | stimulatory at 0.01 mM, inhibitory above 0.1 mM | Caenorhabditis elegans | |
additional information | metalloenzyme, enzyme which is free of metals due to EDTA-treatment cannot be reactivated by addition of Co2+, Zn2+, or Mn2+ | Caenorhabditis elegans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
x * 71000, recombinant His-tagged fusion protein, SDS-PAGE, x * 68000, W03G9.4 protein, SDS-PAGE | Caenorhabditis elegans |
71000 | - |
x * 71000, recombinant His-tagged fusion protein, SDS-PAGE, x * 68000, W03G9.4 protein, SDS-PAGE | Caenorhabditis elegans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli | Caenorhabditis elegans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | expression of GFP-fusion protein | Caenorhabditis elegans | - |
pharynx | expression of GFP-fusion protein | Caenorhabditis elegans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bradykinin + H2O | i.e. RPPGFSPFR, hydrolysis of the N-terminal Arg1-Pro2 bond | Caenorhabditis elegans | Arg + des-Arg-bradykinin | i.e. PPGFSPFR | ? | |
KPSFVRFamide + H2O | a neuropeptide | Caenorhabditis elegans | Lys + PSFVRFamide | - |
? | |
LemTRP-1 + H2O | i.e. APSGFLGVRamide | Caenorhabditis elegans | Ala + PSGFLGVRamide | - |
? | |
additional information | no activity with angiotensin I, des-Arg-bradykinin, AF1, i.e. KNEFIRNRVYIHPFHL, and substance P | Caenorhabditis elegans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 71000, recombinant His-tagged fusion protein, SDS-PAGE, x * 68000, W03G9.4 protein, SDS-PAGE | Caenorhabditis elegans |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase P | - |
Caenorhabditis elegans |
AP-P | - |
Caenorhabditis elegans |
More | the enzyme belongs to the peptidase family M24 | Caenorhabditis elegans |
X-Pro aminopeptidase | - |
Caenorhabditis elegans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Caenorhabditis elegans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | with substrate bradykinin | Caenorhabditis elegans |