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Literature summary for 3.4.13.18 extracted from
- Separation and characterization of two carnosine-splitting cytosolic dipeptidases from hog kidney (carnosinase and non-specific dipeptidase) (1990), Biol. Chem. Hoppe-Seyler, 371, 433-440.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DTT | activates | Sus scrofa |  |
General Stability
| General Stability | Organism |
|---|---|
| DTT stabilizes | Sus scrofa |
| Mn2+ stabilizes | Sus scrofa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| amastatin | - |
Sus scrofa | |
| bestatin | - |
Sus scrofa | |
| Gly-Leu | competitive inhibitor of L-Ala-Gly hydrolysis | Sus scrofa | |
| leucine | - |
Sus scrofa | |
| p-hydroxymercuribenzoate | - |
Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5 | - |
carnosine | - |
Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Sus scrofa | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required | Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
hog | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Sus scrofa | - |
| kidney | - |
Sus scrofa | - |
| liver | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Ala + H2O | - |
Sus scrofa | Ala + Ala | - |
? | |
| Ala-Leu + H2O | - |
Sus scrofa | Ala + Leu | - |
? | |
| carnosine + H2O | - |
Sus scrofa | ? | - |
? | |
| Gly-Gly + H2O | - |
Sus scrofa | Gly + Gly | - |
? | |
| Gly-His + H2O | - |
Sus scrofa | Gly + His | - |
? | |
| Gly-Leu + H2O | - |
Sus scrofa | Gly + Leu | - |
? | |
| His-Ala + H2O | - |
Sus scrofa | His + Ala | - |
? | |
| Leu-Leu + H2O | - |
Sus scrofa | Leu + Leu | - |
? | |
| additional information | not: anserine | Sus scrofa | ? | - |
? | |
| additional information | not: Gly-Gly-Gly | Sus scrofa | ? | - |
? | |
| additional information | not: N-Ac-Met | Sus scrofa | ? | - |
? | |
| additional information | not: homocarnosine | Sus scrofa | ? | - |
? | |
| additional information | not: beta-Ala-Lys | Sus scrofa | ? | - |
? | |
| additional information | not: Gly-His-Gly | Sus scrofa | ? | - |
? | |
| Pro-Ala + H2O | - |
Sus scrofa | Pro + Ala | - |
? | |
| Pro-Leu + H2O | - |
Sus scrofa | Pro + Leu | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Mn2+ stabilizes against heat inactivation | Sus scrofa |
| additional information | - |
DTT stabilizes against heat inactivation | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 5.0 | Sus scrofa |
| 8.8 | - |
carnosine | Sus scrofa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 8.3 | maximal stability at 50°C for 30 min | Sus scrofa |
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