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Literature summary for 3.4.14.10 extracted from
- Purification, substrate specificity, and classification of tripeptidyl peptidase II (1986), J. Biol. Chem., 261, 2409-2417.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Homo sapiens |  |
| 2,2'-Dithiopyridine | strong | Homo sapiens | |
| Acetyl-Val-Leu-Leu-Arg-Ala-Ser-Val-Ala | weak | Homo sapiens | |
| Cu2+ | strong | Homo sapiens | |
| Diazoacetyl norleucine methyl ester | weak | Homo sapiens | |
| diisopropyl fluorophosphate | strong, Val-Leu-Leu-Arg-Ala-Ser-Val-Ala protects | Homo sapiens | |
| Hg2+ | strong, reversible by dialysis against 2 mM DTT | Homo sapiens | |
| IAA | - |
Homo sapiens | |
| iodoacetate | - |
Homo sapiens | |
| additional information | no inhibition by Val-Leu-Arg, isovaleryl-Val-Leu-Arg-Arg-Ala-Ser-Val-Ala, EDTA (with or without DTT) | Homo sapiens | |
| NEM | strong | Homo sapiens | |
| PCMB | - |
Homo sapiens | |
| pepstatin | weak | Homo sapiens | |
| PMSF | strong, irreversible, Val-Leu-Leu-Arg-Ala-Ser-Val-Ala protects | Homo sapiens | |
| Soybean trypsin inhibitor | weak | Homo sapiens | |
| trans-epoxysuccinyl-L-leucinamido(4-guanidino)butane | i.e. E-64, weak | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
Arg-Arg-Ala-(phospho)Ser-Val-Ala | - |
Homo sapiens | |
| 0.016 | - |
Ala-Ala-Phe 4-methylcoumarin 7-amide | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 135000 | - |
x * 135000, SDS-PAGE under reducing conditions | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood | - |
Rattus norvegicus | - |
| erythrocyte | - |
Homo sapiens | - |
| heart | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
| additional information | tissue distribution | Rattus norvegicus | - |
| skeletal muscle | - |
Rattus norvegicus | - |
| spleen | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.245 | - |
- |
Homo sapiens |
| 5.4 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, frozen in liquid nitrogen, stable | Homo sapiens |
| -70°C, frozen in liquid nitrogen, stable | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Ala-Phe 2-naphthylamide + H2O | at 41% the rate of Ala-Ala-Phe 7-amido-4-methylcoumarin | Homo sapiens | Ala-Ala-Phe + 2-naphthylamine | - |
? | |
| Ala-Ala-Phe 7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus | Ala-Ala-Phe + 7-amino-4-methylcoumarin | - |
? | |
| Ala-Ala-Phe 7-amido-4-methylcoumarin + H2O | best chromogenic tripeptyl substrate | Homo sapiens | Ala-Ala-Phe + 7-amino-4-methylcoumarin | - |
? | |
| Arg-Ala-Ser-Val-Ala + H2O | - |
Homo sapiens | Arg-Ala-Ser + Val-Ala | - |
? | |
| Arg-Arg-Ala-(phospho)Ser-Val-Ala + H2O | - |
Homo sapiens | Arg-Arg-Ala + (phospho)Ser-Val-Ala | - |
? | |
| Arg-Arg-Ala-(phospho)Ser-Val-Ala + H2O | - |
Rattus norvegicus | Arg-Arg-Ala + (phospho)Ser-Val-Ala | - |
? | |
| Arg-Arg-Ala-Ser-Val + H2O | - |
Rattus norvegicus | Arg-Arg-Ala + Ser-Val | - |
? | |
| His-Leu-His 2-naphthylamide + H2O | at 12% the rate of Ala-Ala-Phe 4-methylcoumarin 7-amide | Homo sapiens | His-Leu-His + 2-naphthylamine | - |
? | |
| additional information | no substrate: Arg-Arg-Pro-Ser-Val | Rattus norvegicus | ? | - |
? | |
| additional information | no substrate: Pro-Lys-Ala 2-naphthylamide | Homo sapiens | ? | - |
? | |
| Phe-Pro-Ala 2-naphthylamide + H2O | at 6% the rate of Ala-Ala-Phe 4-methylcoumarin 7-amide | Homo sapiens | Phe-Pro-Ala + 2-naphthylamine | - |
? | |
| Val-Gly-Ala-His-Ala-Gly-Glu-Tyr-Gly-Ala-Glu-Ala-Leu-Glu-Arg + H2O | peptide derived from human hemoglobin alpha-chain, residues 17-31, sequential release of tripeptides from free N-terminus, cleaved into 5 tripeptides by human enzyme, cleavage of Gly25-Ala bond occurs at a lower rate than Ala19-His and Gly22-Glu | Homo sapiens | Val-Gly-Ala + His-Ala-Gly + Glu-Tyr-Gly + Ala-Glu-Ala + Leu-Glu-Arg | - |
? | |
| Val-Leu-Arg-Arg-Ala-Ser-Val-Ala + H2O | - |
Homo sapiens | Val-Leu-Arg + Arg-Ala-Ser-Val-Ala | the latter product is cleaved at a higher rate than the substrate | ? | |
| Val-Tyr-Ser 2-naphthylamide + H2O | at 3% the rate of Ala-Ala-Phe 4-methylcoumarin 7-amide | Homo sapiens | Val-Tyr-Ser + 2-naphthylamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Rattus norvegicus |
| ? | x * 135000, SDS-PAGE under reducing conditions | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
hydrolysis of Ala-Ala-Phe 4-methylcoumarin 7-amide | Homo sapiens |
| 7.5 | - |
hydrolysis of Arg-Arg-Ala-(phospho)Ser-Val-Ala | Homo sapiens |
| 7.5 | - |
hydrolysis of Arg-Arg-Ala-(phospho)Ser-Val-Ala | Rattus norvegicus |
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