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Literature summary for 3.4.14.10 extracted from

  • Renn, S.C.; Tomkinson, B.; Taghert, P.H.
    Characterization and cloning of tripeptidyl peptidase II from the fruit fly, Drosophila melanogaster (1998), J. Biol. Chem., 273, 19173-19182.
    View publication on PubMed
Search for more literature for 3.4.14.10

Cloned(Commentary)

Cloned (Comment) Organism
-
 Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
butabindide 0.002 mM, 83% inhibition Drosophila melanogaster
butabindide
-
 Rattus norvegicus
NEM 1 mM, 87% inhibition Drosophila melanogaster
PCMB 0.2 mM, 97% inhibition Drosophila melanogaster
PMSF 1 mM nearly complete inactivation Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane cholecystokinin-8-inactivating peptidase is a membrane bound isoform Rattus norvegicus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 gel filtration Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
cerebellum
-
 Rattus norvegicus
-
embryo higher specific activity in extracts from embryos as compared with adult Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Ala-p-nitroanilide + H2O 24% of the activity with Ala-Ala-Phe-p-nitroanilide Drosophila melanogaster Ala-Ala-Ala + p-nitroaniline
-
 ?
Ala-Ala-Phe-p-nitroanilide + H2O
-
 Drosophila melanogaster Ala-Ala-Phe + p-nitroaniline
-
 ?
Gly-Val-Leu-Arg-Arg-Ala-Ser-Val-Ala + H2O
-
 Drosophila melanogaster Gly-Val-Leu + Arg-Arg-Ala-Ser-Val-Ala
-
 ?
Pro-Pro-Ala-p-nitroanilide + H2O 12% of the activity with Ala-Ala-Phe-p-nitroanilide Drosophila melanogaster Pro-Pro-Ala-p-nitroaniline
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 7.8 reaction with Ala-Ala-Phe-p-nitroanilide Drosophila melanogaster

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0001
-
 butabindide pH 7.5, 37°C Drosophila melanogaster