Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bestatin | 1 mM, 12% inhibition of DPP II-S, 21% inhibition of DPP II-M; no inhibition | Rattus norvegicus | |
diisopropyl fluorophosphate | - |
Rattus norvegicus | |
additional information | no inhibition by bacitracin | Rattus norvegicus | |
N6-benzyloxycarbonyl-Lys-Pro | 1 mM, 31% inhibition of DPP II-S, 41% inhibition of DPP II-M | Rattus norvegicus | |
Na+ | 400 mM NaCl, 56% inhibition of DPP II-M, 31% inhibition of DPP II-S | Rattus norvegicus | |
p-hydroxymercuribenzoate | - |
Rattus norvegicus | |
phenylmethylsulfonyl fluoride | - |
Rattus norvegicus | |
Tris | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
Gly-Pro-4-nitroanilide | enzyme form DPP II-S | Rattus norvegicus | |
0.032 | - |
Gly-Pro-4-nitroanilide | enzyme form DPP II-M | Rattus norvegicus | |
0.068 | - |
Arg-Pro-4-nitroanilide | enzyme form DPP II-M | Rattus norvegicus | |
0.087 | - |
Lys-Ala-2-naphthylamide | enzyme form DPP II-S | Rattus norvegicus | |
0.21 | - |
Lys-Ala-2-naphthylamide | enzyme form DPP II-M | Rattus norvegicus | |
0.502 | - |
His-Pro-Val | enzyme form DPP II-S | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | enzyme form DPP II-S | Rattus norvegicus | 16020 | - |
soluble | enzyme form DPP II-M | Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52500 | - |
2 * 52500, SDS-PAGE | Rattus norvegicus |
110000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
two enzyme forms: DPP II-M and DPP II-S | - |
Purification (Comment) | Organism |
---|---|
enzyme form DPP II-S and DPP II-M | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
hypophysis | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-20°C, pH 6.0, 20 mM BisTris-HCl buffer, stable | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala-2-naphthylamide + H2O | - |
Rattus norvegicus | Ala-Ala + 2-naphthylamine | - |
? | |
Arg-Pro-4-nitroanilide + H2O | - |
Rattus norvegicus | Arg-Pro + 4-nitroaniline | - |
? | |
bradykinin fragment 1-3 + H2O | - |
Rattus norvegicus | ? | - |
? | |
bradykinin fragment 1-5 + H2O | - |
Rattus norvegicus | ? | - |
? | |
Gly-Pro-4-nitroanilide + H2O | - |
Rattus norvegicus | Gly-Pro + 4-nitroaniline | - |
? | |
His-Pro-Val + H2O | - |
Rattus norvegicus | His-Pro + Val | - |
? | |
Lys-Ala-2-naphthylamide + H2O | - |
Rattus norvegicus | Lys-Ala + 2-naphthylamine | - |
? | |
additional information | no activity with bradykinin | Rattus norvegicus | ? | - |
? | |
Phe-Pro-2-naphthylamide + H2O | - |
Rattus norvegicus | Phe-Pro + 2-naphthylamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 52500, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
DPP II | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
hydrolysis of Gly-Pro-4-nitroanilide or Arg-Pro-4-nitroanilide | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
4°C, 24 h, loss of activity of DPP II-M | Rattus norvegicus |
4 | 8 | 4°C, 24 h, DPP II-M is stable | Rattus norvegicus |