Activating Compound | Comment | Organism | Structure |
---|---|---|---|
aminoethyl-benzene sulfonyl fluoride hydrochloride | 1 mM, 18% activation | Streptococcus suis | |
N-(trans-epoxysuccinyl)-L-leucine-4-guanidinobutylamide | 0.01 mM, 25% activation | Streptococcus suis |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Streptococcus suis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 10 mM, 34% inhibition | Streptococcus suis | |
3,4-dichloroisocoumarin | 0.1 mM, 18% inhibition | Streptococcus suis | |
Diprotin A | 1 mM, 71% inhibition | Streptococcus suis | |
Hg2+ | 1 mM, 70% inhibition | Streptococcus suis | |
iodoacetamide | 0.01 mM, 19% inhibition | Streptococcus suis | |
SDS | 35 mM, complete inhibition | Streptococcus suis | |
Triton X-100 | 16 mM, 22% inhibition | Streptococcus suis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
Gly-Pro-4-nitroanilide | - |
Streptococcus suis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus suis | Q673U2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus suis |
Storage Stability | Organism |
---|---|
-20°C, stable to prolonged storage | Streptococcus suis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala-4-nitroanilide + H2O | 36% of the activity with Gly-Pro-4-nitroanilide | Streptococcus suis | Ala-Ala + 4-nitroaniline | - |
? | |
Ala-Pro-4-nitroanilide + H2O | 80% of the activity with Gly-Pro-4-nitroanilide | Streptococcus suis | Ala-Pro + 4-nitroaniline | - |
? | |
Arg-Pro-4-nitroanilide + H2O | 42% of the activity with Gly-Pro-4-nitroanilide | Streptococcus suis | Arg-Pro + 4-nitroaniline | - |
? | |
Asp-Pro-4-nitroanilide + H2O | 11% of the activity with Gly-Pro-4-nitroanilide | Streptococcus suis | Asp-Pro + 4-nitroaniline | - |
? | |
Gly-Pro-4-nitroanilide + H2O | - |
Streptococcus suis | Gly-Pro + 4-nitroaniline | - |
? | |
Lys-Pro-4-nitroanilide + H2O | 51% of the activity with Gly-Pro-4-nitroanilide | Streptococcus suis | Lys-Pro + 4-nitroaniline | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Streptococcus suis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | 45 | 24°C: 52% of maximal activity, 45°C: 78% of maximal activity | Streptococcus suis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
24 h, 86% loss of activity, diluted preparation of recombinant enzyme | Streptococcus suis |
10 | - |
24 h, 92% loss of activity, diluted preparation of recombinant enzyme | Streptococcus suis |
24 | - |
24 h, 93% loss of activity, diluted preparation of recombinant enzyme | Streptococcus suis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Streptococcus suis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6: 61% of maximal activity, pH 9: 54% of maximal activity | Streptococcus suis |