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Literature summary for 3.4.15.1 extracted from

  • Garats, E.V.; Nikolskaya, II; Binevski, P.V.; Pozdnev, V.F.; Kost, O.A.
    Characterization of bovine atrial angiotensin-converting enzyme (2001), Biochemistry (Moscow), 66, 429-434.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Cl- activates Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
captopril
-
Bos taurus
lisinopril
-
Bos taurus
phosphoramidon weak, IC50: 0.001 mM Bos taurus
thiorphan weak, IC50: 0.0001 mM Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
N-(3-(2-furyl)acryloyl)-Phe-Phe-Arg pH 7.5, 25°C Bos taurus
0.14
-
benzyloxycarbonyl-Phe-His-Leu pH 7.5, 25°C Bos taurus
0.98
-
N-(3-(2-furyl)acryloyl)-Phe-Gly-Gly pH 7.5, 25°C Bos taurus
1.8
-
N-hippuryl-His-Leu pH 7.5, 25°C Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
x * 180000, electrophoresis under denaturing conditions Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
atrial tissue
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.37
-
-
Bos taurus

Storage Stability

Storage Stability Organism
-20°C, 90% loss of activity after 1 month Bos taurus
4°C, pH 7.2, in presence of 10% glycerol, activity remains unchanged for at least 3 months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Phe-His-Leu + H2O
-
Bos taurus ?
-
?
N-(3-(2-furyl)acryloyl)-L-Phe-Gly-Gly + H2O
-
Bos taurus 2-furylacrylic acid + L-Phe-Gly-Gly
-
?
N-(3-(2-furyl)acryloyl)-L-Phe-Phe-Arg + H2O
-
Bos taurus 2-furylacrylic acid + L-Phe-Phe-Arg
-
?
N-hippuryl-His-Leu + H2O
-
Bos taurus hippuric acid + His-Leu
-
?

Subunits

Subunits Comment Organism
? x * 180000, electrophoresis under denaturing conditions Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5
-
N-hippuryl-His-Leu pH 7.5, 25°C Bos taurus
30
-
N-(3-(2-furyl)acryloyl)-Phe-Phe-Arg pH 7.5, 25°C Bos taurus
100
-
benzyloxycarbonyl-Phe-His-Leu pH 7.5, 25°C Bos taurus
315
-
N-(3-(2-furyl)acryloyl)-Phe-Gly-Gly pH 7.5, 25°C Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
reaction with benzyloxycarbonyl-Phe-His-Leu Bos taurus
8.3
-
reaction with N-hippuryl-His-Leu Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 pH 6.0: about 40% of maximal activity, pH 8.5: about 35% of maximal activity, reaction with benzyloxycarbonyl-Phe-His-Leu Bos taurus
7 9.5 pH 7.0: about 40% of maximal activity, pH 9.5: about 50% of maximal activity, reaction with N-hippuryl-His-Leu Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000003
-
captopril pH 7.5, 25°C Bos taurus
0.0000006
-
lisinopril pH 7.5, 25°C Bos taurus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0001
-
weak, IC50: 0.0001 mM Bos taurus thiorphan
0.001
-
weak, IC50: 0.001 mM Bos taurus phosphoramidon