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Literature summary for 3.4.15.1 extracted from

  • Bingham, R.J.; Dive, V.; Phillips, S.E.; Shirras, A.D.; Isaac, R.E.
    Structural diversity of angiotensin-converting enzyme (2006), FEBS J., 273, 362-373.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
benzyloxycarbonyl-PhePSI[PO2-CH]Ala-Ala
-
Drosophila sp. (in: flies)
benzyloxycarbonyl-PhePSI[PO2-CH]Ala-Trp
-
Drosophila sp. (in: flies)
NaCl at pH 7 increasing concentrations of NaCl result in a faster rate of conversion which reaches a plateau at 150-200 mM NaCl. The activation by NaCl at pH 7 is the result of a 330% increase in kcat/Km which is solely attributable to a lowering of the Km. At pH 8, maximal activity is achieved in absence of NaCl, weak inhibitory effect on hydrolysis of angiotensin I as salt concentrations increase from 0 to 200 mM Drosophila sp. (in: flies)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.073
-
Arg-Pro-Pro-Gly-Phe-Thr-Pro-Phe-Arg 35°C, pH 8.0 Drosophila sp. (in: flies)
0.27
-
bradykinin 35°C, pH 8.0 Drosophila sp. (in: flies)
0.3
-
Ile-Ser-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg 35°C, pH 8.0 Drosophila sp. (in: flies)
0.37
-
MKRSRGPSPRR 35°C, pH 8.0 Drosophila sp. (in: flies)
0.58
-
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Tyr-Arg 35°C, pH 8.0 Drosophila sp. (in: flies)
0.82
-
angiotensin I 35°C, pH 7.0, 100 mM NaCl Drosophila sp. (in: flies)
1.04
-
angiotensin I 35°C, pH 8.0, 100 mM NaCl Drosophila sp. (in: flies)
1.23
-
angiotensin I 35°C, pH 8.0, 0 mM NaCl Drosophila sp. (in: flies)
2.7
-
angiotensin I 35°C, pH 7.0, 0 mM NaCl Drosophila sp. (in: flies)

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ at pH 7 increasing concentrations of NaCl result in a faster rate of conversion which reaches a plateau at 150-200 mM NaCl. The activation by NaCl at pH 7 is the result of a 330% increase in kcat/Km which is solely attributable to a lowering of the Km. At pH 8, maximal activity is achieved in absence of NaCl, weak inhibitory effect on hydrolysis of angiotensin I as salt concentrations increase from 0 to 200 mM Drosophila sp. (in: flies)

Organism

Organism UniProt Comment Textmining
Drosophila sp. (in: flies)
-
recombinantly expressed in Pichia pastoris
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Drosophila sp. (in: flies)

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin I + H2O
-
Drosophila sp. (in: flies) angiotensin II + His-Leu
-
?
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Tyr-Arg + H2O
-
Drosophila sp. (in: flies) ?
-
?
Arg-Pro-Pro-Gly-Phe-Thr-Pro-Phe-Arg + H2O
-
Drosophila sp. (in: flies) ?
-
?
bradykinin + H2O i.e. Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg Drosophila sp. (in: flies) Arg-Pro-Pro-Gly-Phe + Ser-Pro + Phe-Arg
-
?
Ile-Ser-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
-
Drosophila sp. (in: flies) ?
-
?
MKRSRGPSPRR + H2O
-
Drosophila sp. (in: flies) ?
-
?
[Leu5]enkephalin + H2O
-
Drosophila sp. (in: flies) ?
-
?
[Leu5]enkephalinamide + H2O
-
Drosophila sp. (in: flies) ?
-
?
[Met5]enkephalin + H2O
-
Drosophila sp. (in: flies) ?
-
?
[Met5]enkephalinamide + H2O
-
Drosophila sp. (in: flies) ?
-
?

Synonyms

Synonyms Comment Organism
ANCE
-
Drosophila sp. (in: flies)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03 0.55 angiotensin I 35°C, pH 7.0, 0 mM NaCl Drosophila sp. (in: flies)
0.057 0.65 angiotensin I 35°C, pH 8.0, 0 mM NaCl Drosophila sp. (in: flies)
0.057 0.65 angiotensin I 35°C, pH 8.0, 100 mM NaCl Drosophila sp. (in: flies)
0.24
-
Arg-Pro-Pro-Gly-Phe-Thr-Pro-Phe-Arg 35°C, pH 8.0 Drosophila sp. (in: flies)
1.09
-
bradykinin 35°C, pH 8.0 Drosophila sp. (in: flies)
1.1
-
Ile-Ser-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg 35°C, pH 8.0 Drosophila sp. (in: flies)
2.41
-
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Tyr-Arg 35°C, pH 8.0 Drosophila sp. (in: flies)
6.83
-
angiotensin I 35°C, pH 7.0, 100 mM NaCl Drosophila sp. (in: flies)
6.84
-
angiotensin I 35°C, pH 7.0, 0 mM NaCl Drosophila sp. (in: flies)
10.78
-
angiotensin I 35°C, pH 8.0, 100 mM NaCl Drosophila sp. (in: flies)
11.06
-
angiotensin I 35°C, pH 8.0, 0 mM NaCl Drosophila sp. (in: flies)
18.8
-
MKRSRGPSPRR 35°C, pH 8.0 Drosophila sp. (in: flies)

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.152
-
benzyloxycarbonyl-PhePSI[PO2-CH]Ala-Trp 35°C, pH 8.0 Drosophila sp. (in: flies)
0.365
-
benzyloxycarbonyl-PhePSI[PO2-CH]Ala-Ala 35°C, pH 8.0 Drosophila sp. (in: flies)