Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzyloxycarbonyl-PhePSI[PO2-CH]Ala-Ala | - |
Drosophila sp. (in: flies) | |
benzyloxycarbonyl-PhePSI[PO2-CH]Ala-Trp | - |
Drosophila sp. (in: flies) | |
NaCl | at pH 7 increasing concentrations of NaCl result in a faster rate of conversion which reaches a plateau at 150-200 mM NaCl. The activation by NaCl at pH 7 is the result of a 330% increase in kcat/Km which is solely attributable to a lowering of the Km. At pH 8, maximal activity is achieved in absence of NaCl, weak inhibitory effect on hydrolysis of angiotensin I as salt concentrations increase from 0 to 200 mM | Drosophila sp. (in: flies) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.073 | - |
Arg-Pro-Pro-Gly-Phe-Thr-Pro-Phe-Arg | 35°C, pH 8.0 | Drosophila sp. (in: flies) | |
0.27 | - |
bradykinin | 35°C, pH 8.0 | Drosophila sp. (in: flies) | |
0.3 | - |
Ile-Ser-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg | 35°C, pH 8.0 | Drosophila sp. (in: flies) | |
0.37 | - |
MKRSRGPSPRR | 35°C, pH 8.0 | Drosophila sp. (in: flies) | |
0.58 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Tyr-Arg | 35°C, pH 8.0 | Drosophila sp. (in: flies) | |
0.82 | - |
angiotensin I | 35°C, pH 7.0, 100 mM NaCl | Drosophila sp. (in: flies) | |
1.04 | - |
angiotensin I | 35°C, pH 8.0, 100 mM NaCl | Drosophila sp. (in: flies) | |
1.23 | - |
angiotensin I | 35°C, pH 8.0, 0 mM NaCl | Drosophila sp. (in: flies) | |
2.7 | - |
angiotensin I | 35°C, pH 7.0, 0 mM NaCl | Drosophila sp. (in: flies) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | at pH 7 increasing concentrations of NaCl result in a faster rate of conversion which reaches a plateau at 150-200 mM NaCl. The activation by NaCl at pH 7 is the result of a 330% increase in kcat/Km which is solely attributable to a lowering of the Km. At pH 8, maximal activity is achieved in absence of NaCl, weak inhibitory effect on hydrolysis of angiotensin I as salt concentrations increase from 0 to 200 mM | Drosophila sp. (in: flies) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila sp. (in: flies) | - |
recombinantly expressed in Pichia pastoris | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Drosophila sp. (in: flies) |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin I + H2O | - |
Drosophila sp. (in: flies) | angiotensin II + His-Leu | - |
? | |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Tyr-Arg + H2O | - |
Drosophila sp. (in: flies) | ? | - |
? | |
Arg-Pro-Pro-Gly-Phe-Thr-Pro-Phe-Arg + H2O | - |
Drosophila sp. (in: flies) | ? | - |
? | |
bradykinin + H2O | i.e. Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg | Drosophila sp. (in: flies) | Arg-Pro-Pro-Gly-Phe + Ser-Pro + Phe-Arg | - |
? | |
Ile-Ser-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O | - |
Drosophila sp. (in: flies) | ? | - |
? | |
MKRSRGPSPRR + H2O | - |
Drosophila sp. (in: flies) | ? | - |
? | |
[Leu5]enkephalin + H2O | - |
Drosophila sp. (in: flies) | ? | - |
? | |
[Leu5]enkephalinamide + H2O | - |
Drosophila sp. (in: flies) | ? | - |
? | |
[Met5]enkephalin + H2O | - |
Drosophila sp. (in: flies) | ? | - |
? | |
[Met5]enkephalinamide + H2O | - |
Drosophila sp. (in: flies) | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ANCE | - |
Drosophila sp. (in: flies) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | 0.55 | angiotensin I | 35°C, pH 7.0, 0 mM NaCl | Drosophila sp. (in: flies) | |
0.057 | 0.65 | angiotensin I | 35°C, pH 8.0, 0 mM NaCl | Drosophila sp. (in: flies) | |
0.057 | 0.65 | angiotensin I | 35°C, pH 8.0, 100 mM NaCl | Drosophila sp. (in: flies) | |
0.24 | - |
Arg-Pro-Pro-Gly-Phe-Thr-Pro-Phe-Arg | 35°C, pH 8.0 | Drosophila sp. (in: flies) | |
1.09 | - |
bradykinin | 35°C, pH 8.0 | Drosophila sp. (in: flies) | |
1.1 | - |
Ile-Ser-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg | 35°C, pH 8.0 | Drosophila sp. (in: flies) | |
2.41 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Tyr-Arg | 35°C, pH 8.0 | Drosophila sp. (in: flies) | |
6.83 | - |
angiotensin I | 35°C, pH 7.0, 100 mM NaCl | Drosophila sp. (in: flies) | |
6.84 | - |
angiotensin I | 35°C, pH 7.0, 0 mM NaCl | Drosophila sp. (in: flies) | |
10.78 | - |
angiotensin I | 35°C, pH 8.0, 100 mM NaCl | Drosophila sp. (in: flies) | |
11.06 | - |
angiotensin I | 35°C, pH 8.0, 0 mM NaCl | Drosophila sp. (in: flies) | |
18.8 | - |
MKRSRGPSPRR | 35°C, pH 8.0 | Drosophila sp. (in: flies) |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.152 | - |
benzyloxycarbonyl-PhePSI[PO2-CH]Ala-Trp | 35°C, pH 8.0 | Drosophila sp. (in: flies) | |
0.365 | - |
benzyloxycarbonyl-PhePSI[PO2-CH]Ala-Ala | 35°C, pH 8.0 | Drosophila sp. (in: flies) |