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Literature summary for 3.4.15.6 extracted from

  • Obst, M.; Oppermann-Sanio, F.B.; Luftmann, H.; Steinbchel, A.
    Isolation of cyanophycin-degrading bacteria, cloning and characterization of an extracellular cyanophycinase gene (cphE) from Pseudomonas anguilliseptica strain BI (2002), J. Biol. Chem., 277, 25096-25105.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli XL1-Blue Pseudomonas anguilliseptica

Inhibitors

Inhibitors Comment Organism Structure
EDTA 49% inhibition at 60 nM, 46% inhibition at 30 nM Pseudomonas anguilliseptica
leupeptin 13% inhibition within the range 0.001 nM to 0.01 nM Pseudomonas anguilliseptica
N-bromo-succinimide 100% inhibition at 5 nM, 99% inhibition at 1 nM Pseudomonas anguilliseptica
pefabloc i.e. 4-[2-aminoethyl]benzenesulfonyl fluoride, 92% inhibition at 80 nM, 13.6% inhibition at 8 nM Pseudomonas anguilliseptica
phenylmethylsulfonyl fluoride 63% inhibition at 10 nM, 28% inhibition at 1 nM Pseudomonas anguilliseptica

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Pseudomonas anguilliseptica
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
monomer, SDS-PAGE Pseudomonas anguilliseptica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[L-Asp(4-Arg)]n + H2O Pseudomonas anguilliseptica
-
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
?
[L-Asp(4-Arg)]n + H2O Pseudomonas anguilliseptica BI
-
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas anguilliseptica Q8KQN8
-
-
Pseudomonas anguilliseptica BI Q8KQN8
-
-

Purification (Commentary)

Purification (Comment) Organism
from culture supernatant of Pseudomonas anguilliseptica cells grown in medium containing cyanophycin granules, anion-exchange chromatography and L-arginine-agarose affinity chromatography. In case of heterologous expression in Escherichia coli the enzyme was not secreted and thus, isolation was done from the cell lysate Pseudomonas anguilliseptica

Renatured (Commentary)

Renatured (Comment) Organism
in-gel renaturation method Pseudomonas anguilliseptica

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant cells grown in medium containing cyanophycin granules Pseudomonas anguilliseptica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[L-Asp(4-Arg)]n + H2O
-
Pseudomonas anguilliseptica [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
?
[L-Asp(4-Arg)]n + H2O
-
Pseudomonas anguilliseptica BI [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
?

Synonyms

Synonyms Comment Organism
CGPase
-
Pseudomonas anguilliseptica
CphE
-
Pseudomonas anguilliseptica
cyanophycin granule polypeptidase
-
Pseudomonas anguilliseptica
cyanophycinase
-
Pseudomonas anguilliseptica
extracellular CGPase
-
Pseudomonas anguilliseptica

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas anguilliseptica calculated from the sequence
-
5.92