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Literature summary for 3.4.15.6 extracted from

  • Sallam, A.; Kast, A.; Przybilla, S.; Meiswinkel, T.; Steinbchel, A.
    Biotechnological process for production of beta-dipeptides from cyanophycin on a technical scale and its optimization (2009), Appl. Environ. Microbiol., 75, 29-38.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
food industry the production of beta-dipeptides from cyanophycin by cyanophycinase is economically important, because di- and tripeptides are more efficiently utilized than intact proteins or free amino acids, have a greater nutritional value, and are better absorbed Pseudomonas alcaligenes

Inhibitors

Inhibitors Comment Organism Structure
4-(2-aminoethyl) benzenesulfonylfluoride cyanophycinase is strongly inhibited Pseudomonas alcaligenes
additional information no inhibition by EDTA; no inhibition by leupeptin Pseudomonas alcaligenes
N-bromosuccinimide total inhibition Pseudomonas alcaligenes
phenylmethylsulfonyl fluoride cyanophycinase is strongly inhibited Pseudomonas alcaligenes

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Pseudomonas alcaligenes
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
SDS-PAGE Pseudomonas alcaligenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[L-Asp(4-Arg)]n + H2O Pseudomonas alcaligenes
-
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
?
[L-Asp(4-Arg)]n + H2O Pseudomonas alcaligenes DIP1
-
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas alcaligenes
-
-
-
Pseudomonas alcaligenes DIP1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
centrifugation, proteins are concentrated and desalted using a cross-flow polyethersulfone cassette with a cutoff point of 30 kDa, a purification method for cyanophycinase is developed which depended on the strong affinity of the enzyme in the crude extract for its insoluble substrate cyanophycin Pseudomonas alcaligenes

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
Pseudomonas alcaligenes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[L-Asp(4-Arg)]n + H2O
-
Pseudomonas alcaligenes [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
?
[L-Asp(4-Arg)]n + H2O 2 g/l citrate, pH 6.5, and 37°C are ideal parameters for cyanophycinase production Pseudomonas alcaligenes [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
?
[L-Asp(4-Arg)]n + H2O
-
Pseudomonas alcaligenes DIP1 [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
?
[L-Asp(4-Arg)]n + H2O 2 g/l citrate, pH 6.5, and 37°C are ideal parameters for cyanophycinase production Pseudomonas alcaligenes DIP1 [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
?

Synonyms

Synonyms Comment Organism
CGPase
-
Pseudomonas alcaligenes
CphEal
-
Pseudomonas alcaligenes
cyanophycinase
-
Pseudomonas alcaligenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Pseudomonas alcaligenes

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 80 20 min, sodium phosphate buffer pH 7.0 Pseudomonas alcaligenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
maximum degradation activity at 50°C and complete inactivation at 68°C Pseudomonas alcaligenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Pseudomonas alcaligenes

pH Range

pH Minimum pH Maximum Comment Organism
5 9 20 min, 30°C Pseudomonas alcaligenes