Cloned (Comment) | Organism |
---|---|
into the vector pET21b | Escherichia coli K-12 |
Protein Variants | Comment | Organism |
---|---|---|
D155A | mutant, constructed for the demonstration of the relative importance of residue Asp155 | Escherichia coli K-12 |
F160A | mutant, constructed for the demonstration of the relative importance of residue Phe160 | Escherichia coli K-12 |
Q422A | mutant, constructed for the demonstration of the relative importance of residue Gln422 | Escherichia coli K-12 |
Q422S | mutant, constructed for the demonstration of the relative importance of residue Gln422 | Escherichia coli K-12 |
R361A | mutant, constructed for the demonstration of the relative importance of residue Arg361 | Escherichia coli K-12 |
R361H | mutant, constructed for the demonstration of the relative importance of residue Arg361 | Escherichia coli K-12 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0178 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine | mutant R361H | Escherichia coli K-12 | |
0.0204 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine | wild type enzyme | Escherichia coli K-12 | |
0.0344 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine | mutant F160A | Escherichia coli K-12 | |
0.116 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine | mutant Q422S | Escherichia coli K-12 | |
0.222 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine | mutant D155A | Escherichia coli K-12 | |
0.237 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine | mutant Q422A | Escherichia coli K-12 | |
0.401 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine | mutant R361A | Escherichia coli K-12 | |
0.675 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | mutant Q422S | Escherichia coli K-12 | |
0.678 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | mutant Q422A | Escherichia coli K-12 | |
0.682 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | mutant wild type enzyme | Escherichia coli K-12 | |
0.712 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | mutant R361H | Escherichia coli K-12 | |
0.744 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | mutant R361A | Escherichia coli K-12 | |
0.913 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | mutant F160A | Escherichia coli K-12 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | P24228 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli K-12 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + H2O | - |
Escherichia coli K-12 | N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanine + D-alanine | - |
? | |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine + H2O | - |
Escherichia coli K-12 | N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanine + D-alanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DD-Carboxypeptidase | - |
Escherichia coli K-12 |
LMM-PBP | - |
Escherichia coli K-12 |
low molecular mass penicillin binding protein | - |
Escherichia coli K-12 |
PBP 4 | - |
Escherichia coli K-12 |
penicillin binding protein 4 | - |
Escherichia coli K-12 |
serine-type-D-Ala-D-Ala carboxypeptidase | - |
Escherichia coli K-12 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Escherichia coli K-12 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.42 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine | mutant R361H | Escherichia coli K-12 | |
2.51 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | mutant F160A | Escherichia coli K-12 | |
2.68 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | mutant R361H | Escherichia coli K-12 | |
2.78 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine | mutant R361A | Escherichia coli K-12 | |
2.87 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | mutant R361A | Escherichia coli K-12 | |
2.91 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine | mutant Q422A | Escherichia coli K-12 | |
2.97 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine | mutant Q422S | Escherichia coli K-12 | |
2.98 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine | mutant D155A | Escherichia coli K-12 | |
3.01 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine | mutant F160A | Escherichia coli K-12 | |
3.02 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | mutant Q422S | Escherichia coli K-12 | |
3.13 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | wild type enzyme | Escherichia coli K-12 | |
3.18 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | mutant Q422A | Escherichia coli K-12 | |
3.47 | - |
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine | wild type enzyme | Escherichia coli K-12 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
activity assay | Escherichia coli K-12 |