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Literature summary for 3.4.16.5 extracted from
- Carboxypeptidase Y (1976), Methods Enzymol., 45, 568-587.
General Stability
| General Stability | Organism |
|---|---|
| urea, 6 M, about 80% of activity is retained after 1 h | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-D-Phe | - |
Saccharomyces cerevisiae |  |
| acetyl-D-Phe ethyl ester | - |
Saccharomyces cerevisiae | |
| acetyl-D-Phe-ethyl ester | - |
Saccharomyces cerevisiae | |
| Acetyl-Phe | - |
Saccharomyces cerevisiae | |
| Ag+ | - |
Saccharomyces cerevisiae | |
| Ba2+ | partial | Saccharomyces cerevisiae | |
| benzyloxycarbonyl-D-Phe | - |
Saccharomyces cerevisiae | |
| benzyloxycarbonyl-D-Phe-D-Leu | - |
Saccharomyces cerevisiae | |
| benzyloxycarbonyl-L-phenylalanine chloromethyl ketone | - |
Saccharomyces cerevisiae | |
| benzyloxycarbonyl-Phe | - |
Saccharomyces cerevisiae | |
| beta-phenyl-L-lactate | - |
Saccharomyces cerevisiae | |
| Beta-phenylpropionate | - |
Saccharomyces cerevisiae | |
| Ca2+ | partial | Saccharomyces cerevisiae | |
| Co2+ | partial | Saccharomyces cerevisiae | |
| Cr2+ | partial | Saccharomyces cerevisiae | |
| Cu+ | partial | Saccharomyces cerevisiae | |
| D-Phe | - |
Saccharomyces cerevisiae | |
| D-Phe ethyl ester | - |
Saccharomyces cerevisiae | |
| Fe2+ | partial | Saccharomyces cerevisiae | |
| Fe3+ | partial | Saccharomyces cerevisiae | |
| Hg2+ | - |
Saccharomyces cerevisiae | |
| iodoacetamide | slight | Saccharomyces cerevisiae | |
| L-amino acids | competitive | Saccharomyces cerevisiae | |
| Leu-Phe | - |
Saccharomyces cerevisiae | |
| Mg2+ | partial | Saccharomyces cerevisiae | |
| Mn2+ | partial | Saccharomyces cerevisiae | |
| NH2-blocked amino acids | - |
Saccharomyces cerevisiae | |
| Ni2+ | partial | Saccharomyces cerevisiae | |
| p-hydroxymercuribenzoate | - |
Saccharomyces cerevisiae | |
| Phe | - |
Saccharomyces cerevisiae | |
| Phe-ethyl ester | - |
Saccharomyces cerevisiae | |
| phenylmethane sulfonylfluoride | - |
Saccharomyces cerevisiae | |
| poly-L-lysine | - |
Saccharomyces cerevisiae | |
| Product analogs | - |
Saccharomyces cerevisiae | |
| substrate analogs | - |
Saccharomyces cerevisiae | |
| trans-cinnamate | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Saccharomyces cerevisiae | |
| 0.075 | - |
Furylacryloyl-Phe ethyl ester | wild-type | Saccharomyces cerevisiae | |
| 0.1 | - |
Benzyloxycarbonyl-Phe-Leu | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 0.13 | - |
benzoyl-Tyr-p-nitroanilide | - |
Saccharomyces cerevisiae | |
| 0.24 | - |
Benzyloxycarbonyl-Phe-Leu | pH 6.0, 25°C | Saccharomyces cerevisiae | |
| 0.36 | - |
benzyloxycarbonyl-Phe-Glu | pH 4.5, 25°C | Saccharomyces cerevisiae | |
| 0.41 | - |
benzyloxycarbonyl-Phe-Glu | pH 5.5, 25°C | Saccharomyces cerevisiae | |
| 0.45 | - |
Hippuryl-beta-phenyl lactate | - |
Saccharomyces cerevisiae | |
| 0.5 | - |
benzyloxycarbonyl-Leu-Phe | benzyloxycarbonyl-Phe-Phe at pH 6.5 | Saccharomyces cerevisiae | |
| 0.56 | - |
benzyloxycarbonyl-Phe-Ala | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 0.67 | - |
benzyloxycarbonyl-Phe-Pro | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 1.2 | - |
acetyl-Phe ethyl ester | pH 8.0, 25°C | Saccharomyces cerevisiae | |
| 1.28 | - |
acetyl-Phe ethyl ester | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 2.4 | - |
Acetyl-Tyr-ethyl ester | - |
Saccharomyces cerevisiae | |
| 10 | - |
benzyloxycarbonyl-Phe-NH2 | pH 8.0, 25°C | Saccharomyces cerevisiae | |
| 11 | - |
benzyloxycarbonyl-Phe-NH2 | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 15 | - |
benzyloxycarbonyl-Phe-NH2 | pH 6.0, 25°C | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme does not require metals for activity | Saccharomyces cerevisiae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
sedimentation equilibrium measurement | Saccharomyces cerevisiae |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 2-chloroethanol | 10% stable at pH 7.0 for at least 15 min | Saccharomyces cerevisiae |
| dioxane | 30% stable at pH 7.0 for at least 15 min | Saccharomyces cerevisiae |
| Ethylene glycol | 60% stable at pH 7.0 for at least 15 min | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
baker's yeast | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 16 residues of glucosamine and about 15% hexose | Saccharomyces cerevisiae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-Phe ethyl ester + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| acetyl-Phe-Leu + H2O | - |
Saccharomyces cerevisiae | acetyl-Phe + Leu | - |
? | |
| Acetyl-Tyr ethyl ester + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| acetyl-Tyr-ethyl ester + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| benzoyl-L-Tyr-4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | benzoyl-Tyr + 4-nitroaniline | - |
? | |
| benzoyl-Tyr-4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | benzoyl-Tyr + 4-nitroaniline | - |
? | |
| Benzyloxycarbonyl-Ala-Leu + H2O | - |
Saccharomyces cerevisiae | Benzyloxycarbonyl-Ala + Leu | - |
? | |
| benzyloxycarbonyl-Glu-Leu + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Glu + Leu | - |
? | |
| benzyloxycarbonyl-Glu-Phe + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Glu + Phe | - |
? | |
| Benzyloxycarbonyl-Glu-Tyr + H2O | - |
Saccharomyces cerevisiae | Benzyloxycarbonyl-Glu + Tyr | - |
? | |
| benzyloxycarbonyl-Gly-Arg + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Gly + Arg | - |
? | |
| benzyloxycarbonyl-Gly-Glu + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Gly + Glu | - |
? | |
| benzyloxycarbonyl-Gly-Gly + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Gly + Gly | - |
? | |
| benzyloxycarbonyl-Gly-Gly-Phe + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Gly-Gly + Phe | - |
? | |
| Benzyloxycarbonyl-Gly-Leu + H2O | - |
Saccharomyces cerevisiae | Benzyloxycarbonyl-Gly + Leu | - |
? | |
| Benzyloxycarbonyl-Gly-Phe + H2O | - |
Saccharomyces cerevisiae | Benzyloxycarbonyl-Gly + Phe | - |
? | |
| benzyloxycarbonyl-Gly-Pro + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Gly + Pro | - |
? | |
| benzyloxycarbonyl-His-Leu + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-His + Leu | - |
? | |
| benzyloxycarbonyl-His-Phe + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-His + Phe | - |
? | |
| benzyloxycarbonyl-His-Tyr + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-His + Tyr | - |
? | |
| benzyloxycarbonyl-Ile-Leu + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Ile + Leu | - |
? | |
| benzyloxycarbonyl-Leu-Leu + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Leu + Leu | - |
? | |
| benzyloxycarbonyl-Leu-Phe + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Leu + Phe | - |
? | |
| benzyloxycarbonyl-Nle-Leu + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Nle + Leu | - |
? | |
| benzyloxycarbonyl-Phe-Ala + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + Ala | - |
? | |
| benzyloxycarbonyl-Phe-Glu + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + Glu | - |
? | |
| benzyloxycarbonyl-Phe-Gly + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + Gly | - |
? | |
| benzyloxycarbonyl-Phe-His + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + His | - |
? | |
| benzyloxycarbonyl-Phe-Leu + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + Leu | - |
? | |
| benzyloxycarbonyl-Phe-NH2 + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| benzyloxycarbonyl-Phe-Phe + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + Phe | - |
? | |
| benzyloxycarbonyl-Phe-Pro + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + Pro | - |
? | |
| benzyloxycarbonyl-Pro-Leu + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Pro + Leu | - |
? | |
| benzyloxycarbonyl-Ser-Leu + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Ser + Leu | - |
? | |
| benzyloxycarbonyl-Val-Leu + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Val + Leu | - |
? | |
| furylacryloyl-Phe ethyl ester + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| hippuryl-beta-phenyl lactate + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| additional information | substrates overview | Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61000, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
pH 6-8, stable for 2 h | Saccharomyces cerevisiae |
| 60 | - |
quick loss of activity above | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Saccharomyces cerevisiae | |
| 2.1 | - |
benzoyl-Tyr-4-nitroanilide | - |
Saccharomyces cerevisiae | |
| 2.2 | - |
benzyloxycarbonyl-Phe-NH2 | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 2.8 | - |
benzyloxycarbonyl-Phe-NH2 | pH 6.0, 25°C | Saccharomyces cerevisiae | |
| 5.3 | - |
benzyloxycarbonyl-Phe-NH2 | pH 8.0, 25°C | Saccharomyces cerevisiae | |
| 15.1 | - |
Benzyloxycarbonyl-Phe-Leu | pH 6.0 | Saccharomyces cerevisiae | |
| 21.2 | - |
Hippuryl-beta-phenyl lactate | - |
Saccharomyces cerevisiae | |
| 105 | - |
Acetyl-Tyr ethyl ester | - |
Saccharomyces cerevisiae | |
| 120 | - |
acetyl-Phe ethyl ester | pH 8.0, 25°C | Saccharomyces cerevisiae | |
| 122 | - |
acetyl-Phe ethyl ester | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 130 | - |
Benzyloxycarbonyl-Phe-Leu | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 140 | - |
benzyloxycarbonyl-Phe-Gly | - |
Saccharomyces cerevisiae | |
| 420 | - |
benzyloxycarbonyl-Phe-Phe | - |
Saccharomyces cerevisiae | |
| 465 | - |
Benzyloxycarbonyl-Ala-Leu | - |
Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
or below, release of C-terminal acidic amino acids | Saccharomyces cerevisiae |
| 7 | - |
around, release of C-terminal basic amino acids | Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
quick loss of activity below | Saccharomyces cerevisiae |
| 5.5 | 8 | 8 h, 25°C, stable | Saccharomyces cerevisiae |
| 6 | 8 | 37°C, stable for 2 h | Saccharomyces cerevisiae |
| 7 | - |
at temperatures above 25°C the enzyme is most stable at pH 7.0 | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no cofactor requirement | Saccharomyces cerevisiae |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
benzyloxycarbonyl-D-Phe-D-Leu | esterase activity | Saccharomyces cerevisiae | |
| 0.3 | - |
benzyloxycarbonyl-D-Phe-D-Leu | hydrolysis of benzyloxycarbonyl-Phe-Leu | Saccharomyces cerevisiae | |
| 0.4 | - |
Phe | esterase activity | Saccharomyces cerevisiae | |
| 0.6 | - |
benzyloxycarbonyl-Phe | esterase activity | Saccharomyces cerevisiae | |
| 0.7 | - |
benzyloxycarbonyl-Phe | hydrolysis of benzyloxycarbonyl-Phe-Leu | Saccharomyces cerevisiae | |
| 0.9 | - |
Leu-Phe | hydrolysis of benzyloxycarbonyl-Phe-Leu | Saccharomyces cerevisiae | |
| 1.3 | - |
benzyloxycarbonyl-D-Phe | esterase activity | Saccharomyces cerevisiae | |
| 1.7 | - |
benzyloxycarbonyl-D-Phe | hydrolysis of benzyloxycarbonyl-Phe-Leu | Saccharomyces cerevisiae | |
| 1.9 | - |
Beta-phenylpropionate | esterase activity | Saccharomyces cerevisiae | |
| 2.6 | - |
Leu-Phe | esterase activity | Saccharomyces cerevisiae | |
| 2.6 | - |
beta-phenyl-L-lactate | esterase activity | Saccharomyces cerevisiae | |
| 3.1 | - |
trans-cinnamate | esterase activity | Saccharomyces cerevisiae | |
| 3.9 | - |
Phe | hydrolysis of benzyloxycarbonyl-Phe-Leu | Saccharomyces cerevisiae | |
| 4.5 | - |
Beta-phenylpropionate | hydrolysis of benzyloxycarbonyl-Phe-Leu | Saccharomyces cerevisiae | |
| 5.3 | - |
D-Phe ethyl ester | hydrolysis of benzyloxycarbonyl-Phe-Leu | Saccharomyces cerevisiae | |
| 5.7 | - |
beta-phenyl-L-lactate | hydrolysis of benzyloxycarbonyl-Phe-Leu | Saccharomyces cerevisiae | |
| 8 | - |
trans-cinnamate | hydrolysis of benzyloxycarbonyl-Phe-Leu | Saccharomyces cerevisiae | |
| 8.3 | - |
Phe ethyl ester | hydrolysis of benzyloxycarbonyl-Phe-Leu | Saccharomyces cerevisiae | |
| 9.1 | - |
Phe ethyl ester | esterase activity | Saccharomyces cerevisiae | |
| 11 | - |
D-Phe ethyl ester | esterase activity | Saccharomyces cerevisiae | |
| 12 | - |
acetyl-D-Phe-ethyl ester | esterase activity | Saccharomyces cerevisiae | |
| 20 | - |
Acetyl-Phe | hydrolysis of benzyloxycarbonyl-Phe-Leu | Saccharomyces cerevisiae | |
| 24 | - |
D-Phe | hydrolysis of benzyloxycarbonyl-Phe-Leu | Saccharomyces cerevisiae | |
| 25 | - |
Acetyl-Phe | esterase activity | Saccharomyces cerevisiae | |
| 30 | - |
acetyl-D-Phe ethyl ester | hydrolysis of benzyloxycarbonyl-Phe-Leu | Saccharomyces cerevisiae | |
| 260 | - |
acetyl-D-Phe | hydrolysis of benzyloxycarbonyl-Phe-Leu | Saccharomyces cerevisiae | |
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