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Literature summary for 3.4.17.18 extracted from
- Carboxypeptidase T (1995), Methods Enzymol., 248, 675-683.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Thermoactinomyces vulgaris |
General Stability
| General Stability | Organism |
|---|---|
| Ca2+ stabilizes against thermal denaturation, contains four binding sites for Ca2+ | Thermoactinomyces vulgaris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Thermoactinomyces vulgaris |  |
| EDTA | - |
Thermoactinomyces vulgaris | |
| additional information | not: phenylmethylsulfonyl fluoride, carbobenzoxy-Ala-Ala-Phe-CH2Cl, Hg2+, p-hydroxymercuribenzoate | Thermoactinomyces vulgaris |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
N-Carbobenzoxy-Ala-Ala-Trp | - |
Thermoactinomyces vulgaris | |
| 0.013 | - |
N-carbobenzoxy-Ala-Ala-Phe | - |
Thermoactinomyces vulgaris | |
| 0.026 | - |
N-Carbobenzoxy-Ala-Ala-Leu | - |
Thermoactinomyces vulgaris | |
| 0.78 | - |
N-Carbobenzoxy-Ala-Ala-Lys | - |
Thermoactinomyces vulgaris | |
| 0.92 | - |
N-Carbobenzoxy-Ala-Ala-Arg | - |
Thermoactinomyces vulgaris | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Thermoactinomyces vulgaris | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | contains four binding sites for Ca2+ | Thermoactinomyces vulgaris | |
| Zn2+ | contains one Zn2+ ion per molecule | Thermoactinomyces vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36930 | - |
calculation from amino acid sequence | Thermoactinomyces vulgaris |
| 38000 | - |
1 * 38000, SDS-PAGE | Thermoactinomyces vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoactinomyces vulgaris | - |
- |
- |
| Thermoactinomyces vulgaris INMI | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoactinomyces vulgaris |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Thermoactinomyces vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,4-dinitrophenyl-Ala-Ala-Arg + H2O | - |
Thermoactinomyces vulgaris | 2,4-dinitrophenyl-Ala-Ala + Arg | - |
? | |
| 2,4-dinitrophenyl-Ala-Ala-Leu-Arg + H2O | - |
Thermoactinomyces vulgaris | 2,4-dinitrophenyl-Ala-Ala-Leu + Arg | - |
? | |
| additional information | cleaves off C-terminal neutral, preferably hydrophobic amino acids, similar to carboxypeptidase A and also arginine and lysine residues that bear cationic groups in their side chains | Thermoactinomyces vulgaris | ? | - |
? | |
| additional information | cleaves off C-terminal neutral, preferably hydrophobic amino acids, similar to carboxypeptidase A and also arginine and lysine residues that bear cationic groups in their side chains | Thermoactinomyces vulgaris INMI | ? | - |
? | |
| N-carbobenzoxy-Ala-Ala-Arg + H2O | - |
Thermoactinomyces vulgaris | N-carbobenzoxy-Ala-Ala + Arg | - |
? | |
| N-carbobenzoxy-Ala-Ala-Arg + H2O | - |
Thermoactinomyces vulgaris INMI | N-carbobenzoxy-Ala-Ala + Arg | - |
? | |
| N-carbobenzoxy-Ala-Ala-Leu + H2O | - |
Thermoactinomyces vulgaris | N-carbobenzoxy-Ala-Ala + Leu | - |
? | |
| N-carbobenzoxy-Ala-Ala-Leu + H2O | - |
Thermoactinomyces vulgaris INMI | N-carbobenzoxy-Ala-Ala + Leu | - |
? | |
| N-carbobenzoxy-Ala-Ala-Lys + H2O | - |
Thermoactinomyces vulgaris | N-carbobenzoxy-Ala-Ala + Lys | - |
? | |
| N-carbobenzoxy-Ala-Ala-Phe-OH + H2O | - |
Thermoactinomyces vulgaris | N-carbobenzoxy-Ala-Ala + Phe | - |
? | |
| N-carbobenzoxy-Ala-Ala-Phe-OH + H2O | - |
Thermoactinomyces vulgaris INMI | N-carbobenzoxy-Ala-Ala + Phe | - |
? | |
| N-carbobenzoxy-Ala-Ala-Trp + H2O | - |
Thermoactinomyces vulgaris | N-carbobenzoxy-Ala-Ala + Trp | - |
? | |
| N-carbobenzoxy-Ala-Ala-Trp + H2O | - |
Thermoactinomyces vulgaris INMI | N-carbobenzoxy-Ala-Ala + Trp | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 38000, SDS-PAGE | Thermoactinomyces vulgaris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
8 h, 1 mM Ca2+, 20% loss of activity | Thermoactinomyces vulgaris |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
N-Carbobenzoxy-Ala-Ala-Trp | - |
Thermoactinomyces vulgaris | |
| 2.6 | - |
N-carbobenzoxy-Ala-Ala-Phe | - |
Thermoactinomyces vulgaris | |
| 5 | - |
N-Carbobenzoxy-Ala-Ala-Lys | - |
Thermoactinomyces vulgaris | |
| 12.5 | - |
N-Carbobenzoxy-Ala-Ala-Leu | - |
Thermoactinomyces vulgaris | |
| 57 | - |
N-Carbobenzoxy-Ala-Ala-Arg | - |
Thermoactinomyces vulgaris | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Thermoactinomyces vulgaris |
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Release 2023.1 (January 2023)
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