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Literature summary for 3.4.19.12 extracted from

  • Falquet, L.; Paquet, N.; Frutiger, S.; Hughes, G.J.; Hoang-Van, K.; Jaton, J.C.
    A human de-ubiquitinating enzyme with both isopeptidase and peptidase activities in vitro (1995), FEBS Lett., 359, 73-77.
    View publication on PubMed
Search for more literature for 3.4.19.12

Inhibitors

Inhibitors Comment Organism Structure
peptides containing either of the cleavage site sequence found in ubiquitin polymers, but not unrelated peptides Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 branched dimeric ubiquitin pH 7.2, 37°C Homo sapiens
0.015
-
 head-to-tail dimeric ubiquitin pH 7.2, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 analytical ultracentrifugation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
branched di-ubiquitin + H2O recombinant substrate from expression in Escherichia coli Homo sapiens ubiquitin
-
 ?
branched dimeric ubiquitin + H2O
-
 Homo sapiens ubiquitin
-
 ?
head-to-tail dimeric ubiquitin + H2O
-
 Homo sapiens ubiquitin
-
 ?
head-to-tail polyubiquitin + H2O recombinant substrate from expression in Escherichia coli Homo sapiens ubiquitin
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 110000, SDS-PAGE, monomeric under reducing conditions Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Homo sapiens