Cloned (Comment) | Organism |
---|---|
isozyme L1: expression of wild-type and mutants in Escherichia coli DH5alphaPRO as His-tagged proteins | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C90S | site-directed mutagenesis, isozyme L1, inactive | Homo sapiens |
C90S | exchange of the active site cysteine | Homo sapiens |
I93M | site-directed mutagenesis, isozyme L1, mutant shows increased risk for Parkinson's disease, 45.6% enzyme activity compared to wild-type | Homo sapiens |
S18Y | site-directed mutagenesis, isozyme L1, mutant shows decreased risk for Parkinson's disease, 112.6% enzyme activity compared to wild-type | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aldehyde product of fatty acid peroxidation | i.e. HNE; modifies isozyme L1 at physiological concentrations of 0.01-0.1 mM and reduces enzyme activity, excess N-acetyl-L-cysteine protects | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00011 | - |
ubiquitin-7-amido-4-methylcoumarin | recombinant mutant I93M isozyme L1, pH 7.8, room temperature | Homo sapiens | |
0.00012 | - |
ubiquitin-7-amido-4-methylcoumarin | recombinant wild-type isozyme L1, pH 7.8, room temperature | Homo sapiens | |
0.00014 | - |
ubiquitin-7-amido-4-methylcoumarin | recombinant mutant S18Y isozyme L1, pH 7.8, room temperature | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | increased activity of neuronal isozyme L1 reduces the risk for Parkinson's disease | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P09936 | isozyme L1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutants of isozyme L1 expressed in Escherichia coli DH5alphaPRO | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | isozyme L1, adult | Homo sapiens | - |
neuron | isozyme L1 | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | increased activity of neuronal isozyme L1 reduces the risk for Parkinson's disease | Homo sapiens | ? | - |
? | |
ubiquitin-7-amido-4-methylcoumarin + H2O | recombinant wild-type isozyme L1 and mutants I93M and S18Y | Homo sapiens | ubiquitin + 7-amino-4-methylcoumarin | - |
? | |
ubiquitin-7-amido-4-methylcoumarin + H2O | fluorogenic synthetic substrate | Homo sapiens | ubiquitin + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | secondary structure analysis of wild-type and mutant isozyme L1 by circular dichroism spectroscopy | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
UCH | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
room temperature, assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0794 | - |
ubiquitin-7-amido-4-methylcoumarin | recombinant mutant I93M isozyme L1, pH 7.8, room temperature | Homo sapiens | |
0.174 | - |
ubiquitin-7-amido-4-methylcoumarin | recombinant wild-type isozyme L1, pH 7.8, room temperature | Homo sapiens | |
0.196 | - |
ubiquitin-7-amido-4-methylcoumarin | recombinant mutant S18Y isozyme L1, pH 7.8, room temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Homo sapiens |