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Literature summary for 3.4.19.12 extracted from
- Ubiquitin C-terminal hydrolase L1 regulates the morphology of neural progenitor cells and modulates their differentiation (2006), J. Cell Sci., 119, 162-171.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| stable expression of HA-tagged wild-type UCH-L1 in murine NIH-3T3 cells, expression of FLAG-tagged or GFP-tagged wild-type UCH-L1 and mutants D30A and C90S in murine neural progenitor NP cells, the expression level is lower in NP cells derived from gad, i.e. gracile axonal dystrophy, mice compared to NP cells from wild-type mice, overview | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C90S | site-directed mutagenesis, the mutant enzyme shows no hydrolase and ubiquitin binding activity | Homo sapiens |
| D30A | site-directed mutagenesis, the mutant enzyme shows highly reduced hydrolase and ubiquitin binding activity | Homo sapiens |
| additional information | gad, i.e. gracile axonal dystrophy, mice testis lack isozyme UCH-L1 | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
| cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Mus musculus | UCH-L1 regulates the morphology of neural progenitor cells and modulates their differentiation, it mediates and enhances neurogenesis in the embryonic brain | ? | - |
? |  |
| additional information | Homo sapiens | UCH-L1 regulates the morphology of neural progenitor cells and modulates their differentiation, it mediates and enhances neurogenesis in the embryonic brain | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
wild-type C57BL/6 and mutant gad, i.e. gracile axonal dystrophy, mice | - |
| Mus musculus | Q9R0P9 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
| brain | embryonic, UCH-L1 expression in the ventricular zone changes during neurogenesis and gliogenesis, high expression level in cortical plate and ventricular zone, overview | Mus musculus | - |
| brain | embryo, expression pattern in the ventricular zone changes between embryonic day 14 and 16, which corresponds to the transition from neurogenesis to gliogenesis. At embryonic day 14, UCH-L1 is highly expressed in the ventricular zone, where neurogenesis actively occurs, whereas its expression is prominent in the cortical plate at embroynic day 16. UCH-L1 is very weakly detected in the ventricular zone at embryonic day 16, which corresponds to the start of gliogenesis. UCH-L1 spatially mediates and enhances neurogenesis in the embryonic brain by regulating progenitor cell morphology | Mus musculus | - |
| cell culture | - |
Mus musculus | - |
| embryo | brain, expression pattern in the ventricular zone changes between embryonic day 14 and 16, which corresponds to the transition from neurogenesis to gliogenesis. At embryonic day 14, UCH-L1 is highly expressed in the ventricular zone, where neurogenesis actively occurs, whereas its expression is prominent in the cortical plate at embroynic day 16. UCH-L1 is very weakly detected in the ventricular zone at embryonic day 16, which corresponds to the start of gliogenesis. UCH-L1 spatially mediates and enhances neurogenesis in the embryonic brain by regulating progenitor cell morphology | Mus musculus | - |
| neural progenitor cell | at least two populations exist in the embryonic brain, cell culture | Mus musculus | - |
| neural progenitor cell | in cultured proliferating NPCs, UCH-L1 is coexpressed with nestin. In differentiating cells, UCH-L1 is highly co-expressed with the early neuronal marker TuJ1 | Mus musculus | - |
| neuron | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | UCH-L1 regulates the morphology of neural progenitor cells and modulates their differentiation, it mediates and enhances neurogenesis in the embryonic brain | Mus musculus | ? | - |
? | |
| additional information | UCH-L1 regulates the morphology of neural progenitor cells and modulates their differentiation, it mediates and enhances neurogenesis in the embryonic brain | Homo sapiens | ? | - |
? | |
| additional information | the enzyme shows de-ubiquinating activity | Mus musculus | ? | - |
? | |
| additional information | the enzyme shows de-ubiquinating activity | Homo sapiens | ? | - |
? | |
| ubiquitin-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | ubiquitin + 7-amino-4-methylcoumarin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ubiquitin C-terminal hydrolase L1 | - |
Mus musculus |
| ubiquitin C-terminal hydrolase L1 | - |
Homo sapiens |
| UCH-L1 | - |
Mus musculus |
| UCH-L1 | - |
Homo sapiens |
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