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Literature summary for 3.4.19.12 extracted from

  • Yi, Y.J.; Manandhar, G.; Sutovsky, M.; Li, R.; Jonakova, V.; Oko, R.; Park, C.S.; Prather, R.S.; Sutovsky, P.
    Ubiquitin C-terminal hydrolase-activity is involved in sperm acrosomal function and anti-polyspermy defense during porcine fertilization (2007), Biol. Reprod., 77, 780-793.
    View publication on PubMed

Application

Application Comment Organism
medicine sperm acrosomal UCHs are involved in sperm-zona pellucida interactions and antipolyspermy defense. Modulation of spermal UCH activity may facilitate the management of polyspermy during in vitro fertilization and provide insights into male fertility. Recombinant UCHs reduce polyspermy and proportionally increase the rate of monospermic fertilization during in vitro fertilization. Oocyte-secreted UCHs are not necessary for antipolyspermy defense during in vitro fertilization Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
Antibody anti-UCH antibodies increase the rate of polyspermy during in vitro fertilization by reducing UCH enzymatic activity Sus scrofa
Ubiquitin aldehyde specific nonpermeating UCH inhibitor, signficantly increases rates of sperm-zona pellucida penetration and polyspermy during in vitro fertilization by reducing UCH enzymatic activity in motile boar spermatozoa. Ipase-T and ubiquitin aldehyde cancel each other out when added concomitantly to the fertilization medium Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm UCHL3 diffusely distributed in the zygotic cytoplasm after fertilization Sus scrofa 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
UCHL1 or UCHL3, Western blot analysis, immunocytochemistry Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
oocyte UCHL1 present in the cortex, UCHL3 is primarly associated with the meiotic spindle of metaphase II ova Sus scrofa
-
Sertoli cell UCHL3 Sus scrofa
-
sperm UCHL3 is localized to the acrosomal surface. UCHL1 is absent from the sperm surface Sus scrofa
-
spermatid UCHL3 mainly localized to the round spermatids (acrosomal cap) and elongating spermatids (caudal manchette and acrosome) Sus scrofa
-
spermatogonium UCHL1 Sus scrofa
-
testis UCHL1 and UCHL3 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information UCHL3 interacts with peripheral acrosomal plasma membrane proteins Sus scrofa ?
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
-
Sus scrofa ubiquitin + 7-amino-4-methylcoumarin
-
?

Synonyms

Synonyms Comment Organism
PGP9.5
-
Sus scrofa
ubiquitin C-terminal hydrolase
-
Sus scrofa
UCHL1
-
Sus scrofa
Uchl3
-
Sus scrofa