Application | Comment | Organism |
---|---|---|
medicine | sperm acrosomal UCHs are involved in sperm-zona pellucida interactions and antipolyspermy defense. Modulation of spermal UCH activity may facilitate the management of polyspermy during in vitro fertilization and provide insights into male fertility. Recombinant UCHs reduce polyspermy and proportionally increase the rate of monospermic fertilization during in vitro fertilization. Oocyte-secreted UCHs are not necessary for antipolyspermy defense during in vitro fertilization | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Antibody | anti-UCH antibodies increase the rate of polyspermy during in vitro fertilization by reducing UCH enzymatic activity | Sus scrofa | |
Ubiquitin aldehyde | specific nonpermeating UCH inhibitor, signficantly increases rates of sperm-zona pellucida penetration and polyspermy during in vitro fertilization by reducing UCH enzymatic activity in motile boar spermatozoa. Ipase-T and ubiquitin aldehyde cancel each other out when added concomitantly to the fertilization medium | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | UCHL3 diffusely distributed in the zygotic cytoplasm after fertilization | Sus scrofa | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
UCHL1 or UCHL3, Western blot analysis, immunocytochemistry | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
oocyte | UCHL1 present in the cortex, UCHL3 is primarly associated with the meiotic spindle of metaphase II ova | Sus scrofa | - |
Sertoli cell | UCHL3 | Sus scrofa | - |
sperm | UCHL3 is localized to the acrosomal surface. UCHL1 is absent from the sperm surface | Sus scrofa | - |
spermatid | UCHL3 mainly localized to the round spermatids (acrosomal cap) and elongating spermatids (caudal manchette and acrosome) | Sus scrofa | - |
spermatogonium | UCHL1 | Sus scrofa | - |
testis | UCHL1 and UCHL3 | Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | UCHL3 interacts with peripheral acrosomal plasma membrane proteins | Sus scrofa | ? | - |
? | |
ubiquitin-7-amido-4-methylcoumarin + H2O | - |
Sus scrofa | ubiquitin + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PGP9.5 | - |
Sus scrofa |
ubiquitin C-terminal hydrolase | - |
Sus scrofa |
UCHL1 | - |
Sus scrofa |
Uchl3 | - |
Sus scrofa |