Activating Compound | Comment | Organism | Structure |
---|---|---|---|
4-hydroxy-2-alkenal | modification of UCH-L1 promotes direct interactions between UCH-L1 and tubulin | Homo sapiens | |
4-hydroxy-2-nonenal | modification of UCH-L1 promotes direct interactions between UCH-L1 and tubulin | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | interactions of mutant or carbonyl-modified UCH-L1 with other proteins constitute one of the causes of not only familial Parkinsons disease, but also sporadic Parkinsons disease. Carbonyl-modified and mutant UCH-L1 share common properties, e.g. adopt a similar aberrant structure, promote tubulin polymerization, show decreased ubiquitin binding, and both increased insolubility and interactions with proteins over 30 kDa compared with the wild-type | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
plasmids containing wild-type UCH-L1 and mutants with or without FLAG tag transiently transfected into Neuro2a, SH-SY5Y or COS-7 cells. NIH-3T3 cells stably expressing human UCH-L1 with a FLAG-HA double tag at the N-terminus. PGEX UCH-L1 vectors transformed into Escherichia coli BL21 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C132S | no effect on protein insolubility or interactions | Homo sapiens |
C152S | binds to monoubiquitin in both 4-hydroxy-2-nonenal-treated cells and untreated cells | Homo sapiens |
C220S | no effect on protein insolubility or interactions | Homo sapiens |
C90S | lacks hydrolase activity but maintains binding affinity for ubiquitin, does not exhibit notably increased insolubility upon 4-hydroxy-2-nonenal-treatment compared with UCH-L1 wild-type, has no effect on the interaction of UCH-L1 and tubulin | Homo sapiens |
D30K | lacks hydrolase activity and binding affinity for ubiquitin, has no effect on the interaction of UCH-L1 and tubulin | Homo sapiens |
H185A | displays increased interactions with tubulin | Homo sapiens |
I93M | mutation in UCH-L1 associated with familial Parkinsons disease, shows increased insolubility and elevated interactions with multiple proteins, promotes tubulin polymerization | Homo sapiens |
R63A | displays increased interactions with tubulin, causes a decrease in tubulin polymerization | Homo sapiens |
S18Y/I93M | increased insolubility | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-propenal | carbonyl modification with 0.1 mM | Homo sapiens | |
4-hydroxy-2-hexenal | carbonyl modification in a dose-dependent manner | Homo sapiens | |
4-hydroxy-2-nonenal | carbonyl modification with 0.01-0.1 mM leads to decreased ubiquitin binding, and both increased insolubility and interactions with proteins over 30 kDa compared with the wild-type | Homo sapiens | |
additional information | no carbonyl modification with 0.1 mM or 0.5 mM methylglyoxal and 0.1 mM or 0.5 mM malondialdehyde | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of GST-tagged UCH-L1 by gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-tubulin + H2O | - |
Homo sapiens | ? | - |
? | |
beta-tubulin + H2O | - |
Homo sapiens | ? | - |
? | |
monoubiquitin + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | wild-type UCH-L1 interacts with multiple proteins over 30 kDa. It shows no interaction with beta-actin | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ubiquitin C-terminal hydrolase L1 | - |
Homo sapiens |
UCH-L1 | - |
Homo sapiens |