Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Adrm1 | recruits Uch37 to the proteasome. Binds the carboxy-terminal tail of Uch37. Following binding, Adrm1 relieves Uch37 autoinhibition, accelerating the hydrolysis of ubiquitin-7-amido-4-methylcoumarin | Homo sapiens |
Application | Comment | Organism |
---|---|---|
additional information | Uch37 is responsible for isopeptidase activity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
Uch37N (amino acids 1-237) expressed from pET21a with a C-terminal 6× histidine tag in Escherichia coli BL21(DE3). HEK293 cells stably expressing Uch37 containing an amino-terminal 2 × HA epitope. Uch37 expressed as a carboxy- or amino-terminal fusion to the Gal4 activation domain or the Gal4 DNA-binding domain. GST-Uch37, GST-Uch37N (1-237), GST-Uch37C (238-329), wild-type 6× His-Flag-Uch37 and mutant C88A subcloned into pBacPAK8 with their respective epitope tags and expressed using the BacPAK baculovirus expression system, expressed in Sf21 insect cells. DNA encoding human Uch37FL, Uch37N (1-237), Adrm1, and the 19S complex subunits S1, S2, S3, S4, S5a, S5b, S6, S6, S7, S8, S9, S10a, S10b, S11, S12, S13, S14, S15 and p55 subcloned into pGAD or pGBD yeast two-hybrid vectors and transformed into the YRG-2 yeast strain | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C88A | is inactive | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ubiquitin vinylsulfone | irreversible inhibitor that covalently modifies the active-site cysteines of DUBs | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | proteasome | Homo sapiens | - |
- |
additional information | proteasome | Bos taurus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | Q9XSJ0 | - |
- |
Homo sapiens | Q9Y5K5 | - |
- |
Purification (Comment) | Organism |
---|---|
on Ni-NTA column | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone marrow | - |
Homo sapiens | - |
liver | - |
Bos taurus | - |
additional information | is highly abundant in the PA700 complex | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | neither Uch37 alone nor the Uch37-Adrm1 or Uch37-Adrm1-S1 complexes can hydrolyse di-ubiquitin efficiently, rather incorporation into the 19S complex is required to enable processing of polyubiquitin chains | Homo sapiens | ? | - |
? | |
ubiquitin-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | ubiquitin + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
deubiquitinating enzyme | - |
Homo sapiens |
deubiquitinating enzyme | - |
Bos taurus |
DUB | - |
Homo sapiens |
DUB | - |
Bos taurus |
ubiquitin carboxy-terminal hydrolase | - |
Homo sapiens |
ubiquitin carboxy-terminal hydrolase | - |
Bos taurus |
UCH37 | - |
Homo sapiens |
UCH37 | - |
Bos taurus |