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Literature summary for 3.4.19.12 extracted from

  • Setsuie, R.; Sakurai, M.; Sakaguchi, Y.; Wada, K.
    Ubiquitin dimers control the hydrolase activity of UCH-L3 (2009), Neurochem. Int., 54, 314-321.
    View publication on PubMed

Application

Application Comment Organism
medicine mono-ubiquitin and ubiquitin dimers may regulate the enzymatic functions of UCH-L1 in vivo Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
FLAG-HA tagged UCH-3 expressed in HeLa cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C95A enhances the interaction of ubiquitin dimers with UCH-L3 Homo sapiens
C95S active site mutant of UCH-L1 Homo sapiens
C95S active site mutant of UCH-L3, enhances the interaction of ubiquitin dimers with UCH-L3 Homo sapiens
C95S retains the affinity to interact with ubiquitin dimers Mus musculus
D33A loses the affinity to interact with ubiquitin dimers. D33A mutant expressing cells do not show any signs of free ubiquitin dimers accumulation Mus musculus
D33A ubiquitin affinity deficient mutant, diminishes the interaction of ubiquitin dimers with UCH-L3 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information in vitro, K48-linked ubiquitin dimers pronouncedly inhibit the hydrolase activity of UCH-L3; mono-ubiquitin, a previously identified interacting protein, inhibits the hydrolase activity of UCH-L1 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens mono-ubiquitin and ubiquitin dimers may regulate the enzymatic function of UCH-L1 in vivo ?
-
?
additional information Homo sapiens mono-ubiquitin and ubiquitin dimers may regulate the enzymatic function of UCH-L3 in vivo ?
-
?
additional information Mus musculus mono-ubiquitin and ubiquitin dimers may regulate the enzymatic functions of UCH-L3, in vivo ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P09936
-
-
Homo sapiens P15374
-
-
Mus musculus Q9JKB1
-
-
Mus musculus Q9R0P9
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity purified Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-
MEF cell
-
Mus musculus
-
additional information 3T3 cell Mus musculus
-
testis
-
Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information mono-ubiquitin and ubiquitin dimers may regulate the enzymatic function of UCH-L1 in vivo Homo sapiens ?
-
?
additional information mono-ubiquitin and ubiquitin dimers may regulate the enzymatic function of UCH-L3 in vivo Homo sapiens ?
-
?
additional information mono-ubiquitin and ubiquitin dimers may regulate the enzymatic functions of UCH-L3, in vivo Mus musculus ?
-
?
additional information UCH-L3 does not function to hydrolyze free ubiquitin dimers Mus musculus ?
-
?
additional information UCH-L3 does not show hydrolase activity toward the K48-linked, K63-linked or linear ubiquitin dimer Homo sapiens ?
-
?
ubiquitin-AMC + H2O
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
ubiquitin carboxy terminal hydrolase-L1
-
Homo sapiens
ubiquitin carboxy terminal hydrolase-L1
-
Mus musculus
ubiquitin carboxy terminal hydrolase-L3
-
Homo sapiens
ubiquitin carboxy terminal hydrolase-L3
-
Mus musculus
UCH-L1
-
Homo sapiens
UCH-L1
-
Mus musculus
UCH-L3
-
Homo sapiens
UCH-L3
-
Mus musculus