Cloned (Comment) | Organism |
---|---|
expression of EGFP-tagged UCHL1 in oocytes | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-amino-2-(4-methylbenzoyl)-6-oxo-6,7-dihydrothieno[2,3-b]pyridine-5-carboxylaic acid | an uncompetitive inhibitor of UCHL1 that binds only to the Michaelis complex and not to free enzyme | Bos taurus | |
LDN-57444 | a reversible, competitive, active site-directed isatin oxime with consistent preference for UCHL1 over UCHL3 by 28fold | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | in oocytes | Bos taurus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 27000, SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
oocyte | subcortical localization of native UCHL1 in bovine oocytes during maturation. Expression of UCHL1 monomer does not change during meiotic maturation of bovine oocytes. Developmental competence is unaffected in parthenogenetic embryos that originated from UCH-L1 inhibitor treated oocytes | Bos taurus | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 27000, SDS-PAGE | Bos taurus |
More | presumptive oligomeric forms of UCHL1 increase in the presence of UCHL1 inhibitors | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
ubiquitin C-terminal hydrolase-L1 | - |
Bos taurus |
UCHL1 | - |
Bos taurus |
General Information | Comment | Organism |
---|---|---|
additional information | activity of UCHL1 stimulates ligase activity of this enzyme in bovine oocytes, resulting in increased protein stabilization or modification through K63-linked polyubiquitination | Bos taurus |
physiological function | UCHL1 promotes disassembly of polyubiquitin chains during meiotic maturation. Conversely, UCHL1 regulates formation of polyubiquitin chains in bovine oocytes | Bos taurus |