Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli under the control of an inducible tac promoter | Thermococcus litoralis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Thermococcus litoralis | |
N-ethylmaleimide | - |
Thermococcus litoralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24747 | - |
4 * 24747, calculated from sequence | Thermococcus litoralis |
80000 | 108000 | gel filtration | Thermococcus litoralis |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitrile | at 50% (v/v) concentrations more than 50% of the initial activity is retained | Thermococcus litoralis |
Ethanol | the enzyme retains stability at concentrations up to 90% (v/v) | Thermococcus litoralis |
isopropanol | at 50% (v/v) concentrations more than 50% of the initial activity is retained | Thermococcus litoralis |
Methanol | the enzyme retains stability at concentrations up to 75% (v/v) | Thermococcus litoralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus litoralis | O07883 | - |
- |
Thermococcus litoralis DSM 5473 | O07883 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus litoralis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.5 | - |
pH 7.5, temperature not specified in the publication | Thermococcus litoralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethyl L-pyroglutamate + H2O | - |
Thermococcus litoralis | L-pyroglutamate + ethanol | - |
? | |
ethyl L-pyroglutamate + H2O | - |
Thermococcus litoralis DSM 5473 | L-pyroglutamate + ethanol | - |
? | |
L-pyroglutamyl-beta-naphthylamide + H2O | - |
Thermococcus litoralis | L-pyroglutamate + 2-naphthylamine | - |
? | |
L-pyroglutamyl-beta-naphthylamide + H2O | - |
Thermococcus litoralis DSM 5473 | L-pyroglutamate + 2-naphthylamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 24747, calculated from sequence | Thermococcus litoralis |
Synonyms | Comment | Organism |
---|---|---|
PCP | - |
Thermococcus litoralis |
pyrrolidone carboxyl peptidase | - |
Thermococcus litoralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermococcus litoralis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | 50°C: about 50% of maximal activity | Thermococcus litoralis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
half-life: approximately 1 h | Thermococcus litoralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Thermococcus litoralis |