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Literature summary for 3.4.19.3 extracted from
- A study of a highly specific pyroglutamyl aminopeptidase type-II from the membrane fraction of bovine brain (1998), Int. J. Biochem. Cell Biol., 30, 115-133.
General Stability
| General Stability | Organism |
|---|---|
| addition of 1% bovine serum albumin to the purified enzyme improves stability with 88% of the activity remaining after 4 days at 4°C and 93% of the activity remaining after 21 days at -80°C | Bos taurus |
| enzyme solubilized by sodium deoxycholate and Triton X-100, -20°C or 4°C, 24 h, more than 50% loss of activity | Bos taurus |
| trypsin solubilized enzyme, no loss of activity after 14 days at 4°C, 20% loss of activity after 14 days at -20°C | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Bos taurus |  |
| 1,7-phenanthroline | - |
Bos taurus | |
| 4,7-phenanthroline | - |
Bos taurus | |
| 4-(2-aminoethyl)-benzenesulfonylfluoride | - |
Bos taurus | |
| 8-hydroxyquinoline | - |
Bos taurus | |
| Cd2+ | - |
Bacillus amyloliquefaciens | |
| Cd2+ | CdSO4 | Bos taurus | |
| Dithiobisnitrobenzoate | - |
Bos taurus | |
| dithiothreitol | - |
Bos taurus | |
| EGTA | - |
Bos taurus | |
| Hg2+ | - |
Bacillus amyloliquefaciens | |
| Hg2+ | HgSO4 | Bos taurus | |
| luteinizing hormone releasing hormone 1-5 | - |
Bos taurus | |
| thioglycolic acid | - |
Bos taurus | |
| Zn2+ | ZnSO4 | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00253 | - |
pyroglutamyl-His-Pro-4-methylcoumarin 7-amide | - |
Bos taurus | |
| 0.4198 | - |
pyroglutamyl-His-Pro-4-methylcoumarin 7-amide | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Bos taurus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CoSO4 | activates | Bos taurus | |
| additional information | metallopeptidase | Bos taurus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 214000 | - |
gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus amyloliquefaciens | - |
- |
- |
| Bacillus subtilis | - |
- |
- |
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0208 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C or 4°C, 24 h, more than 50% loss of activity of the enzyme solubilized by sodium deoxycholate and Triton X-100 | Bos taurus |
| 4°C, 72 h, 10% loss of activity of the enzyme solubilized in CHAPS | Bos taurus |
| 4°C, 72 h, 60% loss of activity of the enzyme solubilized in CHAPS | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-pyroglutamyl-His-Pro-4-methylcoumarin 7-amide + H2O | - |
Bos taurus | L-pyroglutamate + His-Pro-4-methylcoumarin 7-amide | - |
? | |
| pyroglutamyl-4-methylcoumarin 7-amide + H2O | - |
Bos taurus | pyroglutamate + 7-amino-4-methylcoumarin | - |
? | |
| pyroglutamyl-His-Gly + H2O | - |
Bos taurus | pyroglutamate + His-Gly | - |
? | |
| pyroglutamyl-His-Gly-NH2 + H2O | - |
Bos taurus | pyroglutamate + His-Gly-NH2 | - |
? | |
| pyroglutamyl-His-Pro-Gly + H2O | - |
Bos taurus | pyroglutamate + His-Pro-Gly | - |
? | |
| pyroglutamyl-His-Pro-Gly-NH2 + H2O | - |
Bos taurus | pyroglutamate + His-Pro-Gly-NH2 | - |
? | |
| pyroglutamyl-Phe-Pro-NH2 + H2O | - |
Bos taurus | pyroglutamate + Phe-Pro-NH2 | - |
? | |
| thyroliberin + H2O | - |
Bos taurus | pyroglutamate + His-Pro-NH2 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable for at least 1 h | Bos taurus |
| 55 | - |
partial inactivation | Bos taurus |
| 60 | - |
2 min, total inactivation | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.6 | - |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | pH 6.5: about 45% of maximal activity, pH 8.5: about 25% of maximal activity | Bos taurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9.2 | rapid inactivation below pH 4.0 and above pH 9.2 | Bos taurus |
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Release 2023.1 (January 2023)
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