Literature summary for 3.4.19.6 extracted from

Gallagher, S.P.; O'Connor, B.
A study of a highly specific pyroglutamyl aminopeptidase type-II from the membrane fraction of bovine brain (1998), Int. J. Biochem. Cell Biol., 30, 115-133.

Activating Compound

Activating Compound	Comment	Organism	Structure
DMSO	activity using substrates containing 2% v/v DMSO is 5% higher than that using substrate containing no DSMO	Bos taurus

General Stability	Organism
1% w/v bovine serum albumin greatly improves stability with 88% of the activity remaining after 4 days at 4°C and 93% of the activity remaining after 21 days at -80°C	Bos taurus
addition of 20% v/v glyceriol affords no protection of the enzyme stored at 4°C and only moderate protection at -20°C and -80°C	Bos taurus
trypsin is the most effective protease, above 0.001 mg/ml, the solubilized enzyme st stable for 14 days at 4°C, approximately 80% of the activity remains after 14 days at -20°C	Bos taurus

Inhibitors	Comment	Organism
1,7-phenanthroline	10 mM, 67% inhibition	Bos taurus
4,7-phenanthroline	10 mM, 56% inhibition	Bos taurus
4-(2-aminoethyl)-benzenesulfonylfluoride	66% inhibition at 1 mM, 97% inhibition at 10 mM	Bos taurus
8-hydroxyquinoline	10 mM, 68% inhibition	Bos taurus
Cd2+	50% inhibition at 0.1 mM CsSO4, 98% inhibition at 1 mM	Bos taurus
CDTA	10 mM, 10% inhibition	Bos taurus
CuSO4	0.1 mM	Bos taurus
EDTA	10 mM, 29% inhibition	Bos taurus
Hg2+	1 mM HgSO4, 76% inhibition	Bos taurus
Luteinizing hormone releasing hormone	-	Bos taurus
MgSO4	0.1 mM	Bos taurus
NEM	10 mM, 22% inhibition	Bos taurus
puromycin	1 mM, 17% inhibition	Bos taurus
pyroglutamyl-7-amido-4-methylcoumarin	-	Bos taurus
pyroglutamyl-His	-	Bos taurus
Pyroglutamyl-His-Gly	-	Bos taurus
pyroglutamyl-His-Gly-NH2	-	Bos taurus
pyroglutamyl-His-Pro	-	Bos taurus
pyroglutamyl-His-Pro-7-amido-4-methylcoumarin	-	Bos taurus
pyroglutamyl-His-Pro-Gly	-	Bos taurus
pyroglutamyl-His-Pro-Gly-NH2	-	Bos taurus
pyroglutamyl-His-Pro-NH2	-	Bos taurus
pyroglutamyl-His-Trp-Ser-Tyr-Gly	-	Bos taurus
pyroglutamyl-His-Trp-Ser-Tyr-Gly-Leu	-	Bos taurus
pyroglutamyl-His-Trp-Ser-Tyr-Gly-Leu-Arg	-	Bos taurus
pyroglutamyl-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly	-	Bos taurus
pyroglutamyl-Phe-Pro-NH2	-	Bos taurus
ZnSO4	1 mM ZnSO4, 61% inhibition	Bos taurus

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00253	-	pyroglutamyl-His-Pro-7-amido-4-methylcoumarin	37°C	Bos taurus
0.419	-	pyroglutamyl-7-amido-4-methylcoumarin	37°C	Bos taurus

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Bos taurus	16020	-

Metals/Ions	Comment	Organism
CoSO4	161% enhancement of activity at 0.1 mM and 559% enhancement at 1 mM. 1 mM, 587% reactivation of the enzyme inactivated by EDTA	Bos taurus
MnSO4	0.1 mM, 56% reactivation of the enzyme inactivated by EDTA	Bos taurus
NiSO4	1 mM, 185% reactivation of the enzyme inactivated by EDTA	Bos taurus
ZnSO4	0.1 mM, 108% reactivation of the enzyme inactivated by EDTA	Bos taurus

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
214000	-	gel filtration	Bos taurus

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Comment)	Organism
-	Bos taurus

Source Tissue	Comment	Organism	Textmining
brain	-	Bos taurus	-

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0209	-	-	Bos taurus

Storage Stability	Organism
4°C or -20°C, 24 h, decrease of more than 50% of activity of the enzyme solubilized by sodium deoxycholate or Triton X-100, the CHAPS solubilized enzyme is slightly more stable with approximately 90% and 40% of the activity remaining after 72 h at 4°C and -20°C	Bos taurus

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
pyroglutamyl-7-amido-4-methylcoumarin + H2O	-	Bos taurus	pyroglutamate + 7-amino-4-methylcoumarin	-	?
pyroglutamyl-His-Pro-7-amido-4-methylcoumarin + H2O	-	Bos taurus	pyroglutamate + His-Pro-7-amido-4-methylcoumarin	-	?
thyroliberin + H2O	-	Bos taurus	pyroglutamic acid + His-Pro-NH2	-	?

Subunits	Comment	Organism
More	a number of protein bands varying in MW from 55000 to 141000 Da are detected by SDS-PAGE	Bos taurus

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	-	Bos taurus

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
25	56	25°C: 16% of maximal activity, 37°C: 70% of maximal activity, 45°C: maximal activity	Bos taurus

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4	-	24 h, purified enzyme, 60% loss of activity	Bos taurus
45	-	1 h, stable	Bos taurus
55	-	2 min, partial inactivation	Bos taurus
60	-	1 min, complete inactivation	Bos taurus

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.8	7.6	-	Bos taurus

pH Minimum	pH Maximum	Comment	Organism
5.8	8.4	pH 5.8: about 45% of maximal activity, pH 8.4: about 20% of maximal activity	Bos taurus

pH Stability	pH Stability Maximum	Comment	Organism
4	8	37°C, 15 min, stable from pH 4.0-8.0	Bos taurus

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.008	-	Luteinizing hormone releasing hormone	-	Bos taurus
0.0081	-	pyroglutamyl-His-Trp-Ser-Tyr-Gly	37°C	Bos taurus
0.0108	-	pyroglutamyl-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly	37°C	Bos taurus
0.0194	-	pyroglutamyl-His-Trp-Ser-Tyr-Gly-Leu	37°C	Bos taurus
0.0296	-	pyroglutamyl-His-Pro-Gly-NH2	37°C	Bos taurus
0.0403	-	pyroglutamyl-His-Pro-NH2	37°C	Bos taurus
0.0588	-	pyroglutamyl-His-Pro-Gly	37°C	Bos taurus
0.0603	-	pyroglutamyl-His-Trp-Ser-Tyr-Gly-Leu-Arg	37°C	Bos taurus
0.0731	-	pyroglutamyl-Phe-Pro-NH2	37°C	Bos taurus
0.236	-	pyroglutamyl-His-Pro	37°C	Bos taurus
0.582	-	pyroglutamyl-His-Gly-NH2	37°C	Bos taurus
1.67	-	Pyroglutamyl-His-Gly	37°C	Bos taurus