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Literature summary for 3.4.21.25 extracted from

  • Kaneda, M.; Yonezawa, H.; Uchikoba, T.
    Purification and some properties of a protease from the sarcocarp of musk melon fruit (1997), Biosci. Biotechnol. Biochem., 61, 2100-2102.
    View publication on PubMed
Search for more literature for 3.4.21.25

Inhibitors

Inhibitors Comment Organism Structure
4-(2-aminoethyl)-benzenesulfonyl fluoride 17% inhibition by 1 mM; i.e. Pefabloc SC Cucumis melo
5,5'-dithiobis(2-nitrobenzoic acid) 11% inhibition by 1 mM Cucumis melo
diisopropyl fluorophosphate complete inhibition by 2 mM Cucumis melo
additional information no inhibition with N-tosyl-L-lysine chloromethyl ketone, N-tosyl-L-phenylalanine chloromethyl ketone, iodoacetate, PCMB, p-chloromercuribenzenesulfonate, E-64, antipain, ovomucoid, EDTA, soybean trypsin inhibitors Cucumis melo
ZnCl2 11% inhibition by 2 mM Cucumis melo

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.355
-
 Ala-Ala-Pro-Glu-p-nitroanilide
-
 Cucumis melo
0.485
-
 Ala-Ala-Pro-Gly-p-nitroanilide
-
 Cucumis melo
0.585
-
 Ala-Ala-Pro-Ala-p-nitroanilide
-
 Cucumis melo
0.595
-
 Ala-Ala-Pro-Val-p-nitroanilide
-
 Cucumis melo
0.826
-
 Ala-Ala-Pro-Lys-p-nitroanilide
-
 Cucumis melo
0.826
-
 Ala-Ala-Pro-Phe-p-nitroanilide
-
 Cucumis melo
1.11
-
 Ala-Ala-Pro-L-diaminopropionic acid-p-nitroanilide
-
 Cucumis melo
2.96
-
 Ala-Ala-Pro-Leu-p-nitroanilide
-
 Cucumis melo

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected by MgSO4, CaCl2, CoCl2 and MnCl2 Cucumis melo

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Protein + H2O Cucumis melo
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cucumis melo
-
 L. var reticulatus cv. Teresa
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cucumis melo

Source Tissue

Source Tissue Comment Organism Textmining
sarcocarp
-
 Cucumis melo
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Pro-Ala-p-nitroanilide + H2O
-
 Cucumis melo ?
-
 ?
Ala-Ala-Pro-Glu-p-nitroanilide + H2O
-
 Cucumis melo ?
-
 ?
Ala-Ala-Pro-Gly-p-nitroanilide + H2O
-
 Cucumis melo ?
-
 ?
Ala-Ala-Pro-L-diaminopropionic acid-p-nitroanilide + H2O
-
 Cucumis melo ?
-
 ?
Ala-Ala-Pro-Leu-p-nitroanilide + H2O
-
 Cucumis melo ?
-
 ?
Ala-Ala-Pro-Lys-p-nitroanilide + H2O
-
 Cucumis melo ?
-
 ?
Ala-Ala-Pro-Phe-p-nitroanilide + H2O
-
 Cucumis melo ?
-
 ?
Ala-Ala-Pro-Val-p-nitroanilide + H2O
-
 Cucumis melo ?
-
 ?
casein + H2O
-
 Cucumis melo ?
-
 ?
Protein + H2O
-
 Cucumis melo ?
-
 ?
protein + H2O
-
 Cucumis melo hydrolyzed protein
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.022
-
 Ala-Ala-Pro-Val-p-nitroanilide
-
 Cucumis melo
0.043
-
 Ala-Ala-Pro-Gly-p-nitroanilide
-
 Cucumis melo
0.295
-
 Ala-Ala-Pro-Lys-p-nitroanilide
-
 Cucumis melo
0.603
-
 Ala-Ala-Pro-L-diaminopropionic acid-p-nitroanilide
-
 Cucumis melo
1.21
-
 Ala-Ala-Pro-Glu-p-nitroanilide
-
 Cucumis melo
2.51
-
 Ala-Ala-Pro-Ala-p-nitroanilide
-
 Cucumis melo
5.26
-
 Ala-Ala-Pro-Phe-p-nitroanilide
-
 Cucumis melo
23.9
-
 Ala-Ala-Pro-Leu-p-nitroanilide
-
 Cucumis melo

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Cucumis melo

pH Range

pH Minimum pH Maximum Comment Organism
8 11.5 50% of the maximal activity at pH 8 and pH 11.5 Cucumis melo

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 80% of the activity remains after after incubation Cucumis melo