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Literature summary for 3.4.21.59 extracted from

  • Cromlish, J.A.; Seidah, N.G.; Marcinkiewicz, M.; Hamelin, J.; Jonson, D.A.; Chretien, M.
    Human pituitary tryptase: molecular forms, NH2-terminal sequence, immunocytochemical localization, and specificity with prohormone and fluorogenic substrates (1987), J. Biol. Chem., 262, 1363-1373.
    View publication on PubMed
Search for more literature for 3.4.21.59

Inhibitors

Inhibitors Comment Organism Structure
antipain
-
 Homo sapiens
CaCl2
-
 Homo sapiens
D-Tyr-Glu-Phe-Lys-Arg-CH2Cl
-
 Homo sapiens
diisopropyl fluorophosphate
-
 Homo sapiens
DTNB
-
 Homo sapiens
HgCl2
-
 Homo sapiens
leupeptin
-
 Homo sapiens
N-tosyl-L-Lys chloromethyl ketone
-
 Homo sapiens
NaCl reversible at 1 M Homo sapiens
p-chloromercuribenzoate
-
 Homo sapiens
PMSF
-
 Homo sapiens
ZnSO4
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0138
-
 Benzyloxycarbonyl-L-Ala-L-Lys-L-Arg 4-methylcoumarin 7-amide
-
 Homo sapiens
0.0148
-
 Benzyloxycarbonyl-L-Ala-L-Lys-L-Lys 4-methylcoumarin 7-amide
-
 Homo sapiens
0.0409
-
 Benzyloxycarbonyl-L-Ala-L-Arg-L-Arg 4-methylcoumarin 7-amide
-
 Homo sapiens
0.323
-
 benzyloxycarbonyl-L-Ala-L-Leu-L-Lys-7-amido-4-methylcoumarin
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 human, gel filtration in presence of 0.3 M NaCl Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein some of the MW heterogeneity of the multiple forms results from N-linked glycosylation Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
multiple forms Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
mast cell present in pituitary connective tissue Homo sapiens
-

Storage Stability

Storage Stability Organism
-20°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Benzyloxycarbonyl-L-Ala-L-Arg-L-Arg 4-methylcoumarin 7-amide + H2O
-
 Homo sapiens Benzyloxycarbonyl-L-Ala-L-Arg-L-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-L-Ala-L-Leu-L-Lys-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-L-Ala-L-Leu-L-Lys + 7-amino-4-methylcoumarin
-
 ?
Benzyloxycarbonyl-L-Ala-L-Lys-L-Arg 4-methylcoumarin 7-amide + H2O
-
 Homo sapiens Benzyloxycarbonyl-L-Ala-L-Lys-L-Arg + 7-amino-4-methylcoumarin
-
 ?
Benzyloxycarbonyl-L-Ala-L-Lys-L-Lys 4-methylcoumarin 7-amide + H2O
-
 Homo sapiens Benzyloxycarbonyl-L-Ala-L-Lys-L-Lys + 7-amino-4-methylcoumarin
-
 ?
additional information pituitary enzyme has a preference for cleaving COOH-terminal to arginine or lysine residues which are preceded by a proline residue 4 or 6 residues NH2-terminal to the site of cleavage Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 32400-36300, human, SDS-PAGE, various enzyme forms Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1520
-
 Benzyloxycarbonyl-L-Ala-L-Lys-L-Lys 4-methylcoumarin 7-amide
-
 Homo sapiens
1870
-
 benzyloxycarbonyl-L-Ala-L-Leu-L-Lys-7-amido-4-methylcoumarin
-
 Homo sapiens
2040
-
 Benzyloxycarbonyl-L-Ala-L-Arg-L-Arg 4-methylcoumarin 7-amide
-
 Homo sapiens
3290
-
 Benzyloxycarbonyl-L-Ala-L-Lys-L-Arg 4-methylcoumarin 7-amide
-
 Homo sapiens