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Literature summary for 3.4.21.61 extracted from

  • Mizuno, K.; Nakamura, T.; Oshima, T.; Tanaka, S.; Matsuo, H.
    Characterization of KEX2-encoded endopeptidase from yeast Saccharomyces cerevisiae (1989), Biochem. Biophys. Res. Commun., 159, 305-311.
    View publication on PubMed
Search for more literature for 3.4.21.61

Cloned(Commentary)

Cloned (Comment) Organism
cloned KEX2 gene introduced into the kex2 mutant cells and the KEX2 gene product expressed in these cells Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
diisopropyl fluorophosphate
-
 Saccharomyces cerevisiae
HgCl2
-
 Saccharomyces cerevisiae
leupeptin
-
 Saccharomyces cerevisiae
p-chloromercuribenzoate
-
 Saccharomyces cerevisiae
pepstatin A
-
 Saccharomyces cerevisiae
phenylmethylsulfonyl fluoride
-
 Saccharomyces cerevisiae
ZnCl2
-
 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 cloned KEX2 gene introduced into the kex2 mutant cells and the KEX2 gene product expressed in these cells
-

Purification (Commentary)

Purification (Comment) Organism
partial Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-tert-butyloxycarbonyl-Gly-Lys-Arg 4-methylcoumarin 7-amide + H2O 59.2% of the activity with tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide Saccharomyces cerevisiae N-tert-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
Protein + H2O specificity towards the carbonyl side of Lys-Arg, Arg-Arg and Pro-Arg sequences Saccharomyces cerevisiae ?
-
 ?
tert-Butyloxycarbonyl-Ala-Pro-Arg 4-methylcoumarin 7-amide + H2O 17% of the activity with tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide Saccharomyces cerevisiae ?
-
 ?
tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae tert-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
tert-Butyloxycarbonyl-Leu-Arg-Arg 4-methylcoumarin 7-amide + H2O 116% of the activity with tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide Saccharomyces cerevisiae ?
-
 ?
tert-Butyloxycarbonyl-Leu-Lys-Arg 4-methylcoumarin 7-amide + H2O 92.8% of the activity with tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide Saccharomyces cerevisiae ?
-
 ?
tert-Butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O 38% of the activity with tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide Saccharomyces cerevisiae ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide Saccharomyces cerevisiae