Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.65 extracted from

  • Gaucher, G.M.; Stevenson, K.J.
    Thermomycolin (1976), Methods Enzymol., 45, 415-433.
    View publication on PubMed
Search for more literature for 3.4.21.65

General Stability

General Stability Organism
Autolysis, which is the dominant inactivation process below 50°C, is decreased by the presence of calcium or macromolecules Malbranchea pulchella
Denaturation by freeze-drying Malbranchea pulchella
Denaturation by freezing Malbranchea pulchella
No loss of activity after 12 h of agitation on a reciprocal shaker in the absence of silicone antifoam agent or in the presence of both the antifoam agent and 5% Ficoll Malbranchea pulchella
Particularly thermostable in the presence of Ca2+ Malbranchea pulchella
Urea denaturation, 8 M, is markedly inhibited by the binding of a single Ca2+ Malbranchea pulchella

Inhibitors

Inhibitors Comment Organism Structure
4-Methylphenylsulfonyl fluorides
-
 Malbranchea pulchella
4-Nitrophenylsulfonyl fluorides
-
 Malbranchea pulchella
BaCl2 weak Malbranchea pulchella
Carboxybenzoyl halomethyl ketones L-Phe-CH2Cl and L-Phe-CH2Br Malbranchea pulchella
CdCl2
-
 Malbranchea pulchella
CoCl2
-
 Malbranchea pulchella
CuCl2
-
 Malbranchea pulchella
DFP
-
 Malbranchea pulchella
Egg white trypsin inhibitor
-
 Malbranchea pulchella
HgCl2
-
 Malbranchea pulchella
Lima bean trypsin inhibitor
-
 Malbranchea pulchella
MgCl2
-
 Malbranchea pulchella
MnCl2
-
 Malbranchea pulchella
NiCl2
-
 Malbranchea pulchella
Phenylmethylsulfonyl fluorides
-
 Malbranchea pulchella
Soybean trypsin inhibitor
-
 Malbranchea pulchella
ZnCl2
-
 Malbranchea pulchella

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 Acetyl-Ala-Ala-Ala methyl ester
-
 Malbranchea pulchella

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Malbranchea pulchella
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11000 17500 Malbranchea pulchella var. sulfurea, gel filtration Malbranchea pulchella
32000
-
 Malbranchea pulchella var. sulfurea, sedimentation equilibrium measurement Malbranchea pulchella
32700
-
 Malbranchea pulchella var. sulfurea, amino acid analysis Malbranchea pulchella

Organism

Organism UniProt Comment Textmining
Malbranchea pulchella
-
 var. sulfurea
-

Purification (Commentary)

Purification (Comment) Organism
-
 Malbranchea pulchella

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Malbranchea pulchella
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Malbranchea pulchella

Storage Stability

Storage Stability Organism
-20°C Malbranchea pulchella

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Acetyl-Ala-Ala-Ala methyl ester + H2O
-
 Malbranchea pulchella ?
-
 ?
Bovine serum albumin + H2O
-
 Malbranchea pulchella Hydrolyzed bovine serum albumin
-
 ?
Carboxybenzoyl-Gly 4-nitrophenyl ester + H2O
-
 Malbranchea pulchella ?
-
 ?
Carboxybenzoyl-L-Ala 4-nitrophenyl ester + H2O
-
 Malbranchea pulchella ?
-
 ?
Carboxybenzoyl-L-Phe 4-nitrophenyl ester + H2O
-
 Malbranchea pulchella ?
-
 ?
casein + H2O
-
 Malbranchea pulchella hydrolyzed casein
-
 ?
Glucagon + H2O preferential cleavage of Thr5-Phe6, Ser8-Asp9, Lys12-Tyr13, Asp15-Ser16, Ser16-Arg17 and Arg17-Arg18, no amino acids observed in the digest Malbranchea pulchella Hydrolyzed glucagon no amino acids observed in the digest ?
additional information slow release of peptides from native collagen Malbranchea pulchella ?
-
 ?
additional information active site sequence: Leu-Ser-(Gly)-Thr-Ser-Met Malbranchea pulchella ?
-
 ?
additional information specificity towards small synthetic ester substrates: preference in decreasing order for L-Ala, L-Tyr, L-Phe, Gly, L-Leu, L-Trp, L-Val, L-Lys, L-Pro on the carboxyl side of the bond hydrolyzed Malbranchea pulchella ?
-
 ?
additional information no keratinase activity Malbranchea pulchella ?
-
 ?
Oxidized insulin A-chain + H2O no amino acids observed in the digest Malbranchea pulchella Hydrolyzed oxidized insulin A-chain no amino acids observed in the digest ?
Oxidized insulin B-chain + H2O no amino acids observed in the digest Malbranchea pulchella Hydrolyzed oxidized insulin B-chain no amino acids observed in the digest ?

Subunits

Subunits Comment Organism
?
-
 Malbranchea pulchella

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 significant thermostability, especially in the presence of Ca2+ Malbranchea pulchella
70
-
 maximal thermostability 10 mM Ca2+ Malbranchea pulchella
73
-
 t1/2: 7.5 min without Ca2+ Malbranchea pulchella
73
-
 110 min with 10 mM Ca2+ Malbranchea pulchella

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Malbranchea pulchella

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 6.0 Malbranchea pulchella
8.5
-
 casein Malbranchea pulchella
8.5
-
 bovine serum albumin Malbranchea pulchella

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5
-
 rapid and irreversible denaturation below Malbranchea pulchella
4.5 10.5 30°C, 2 h, stable Malbranchea pulchella
6 9.6 30°C, 20 h, stable Malbranchea pulchella
8.5
-
 optimum pH for stability Malbranchea pulchella