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Literature summary for 3.4.21.75 extracted from

  • Jean, F.; Basak, A.; Rondeau, N.; Benjannet, S.; Hendy, G.N.; Seidah, N.G.
    Enzymic characterization of murine and human prohormone convertase-1 (mPC1 and hPC1) expressed in mammalian GH4C1 cells (1993), Biochem. J., 292, 891-900.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.75

Inhibitors

Inhibitors Comment Organism Structure
antipain
-
 Homo sapiens
antipain
-
 Mus musculus
Arg-oxime
-
 Homo sapiens
Arg-oxime
-
 Mus musculus
Ca2+ 100 mM; activation below Homo sapiens
Ca2+ 100 mM; activation below Mus musculus
CDTA
-
 Homo sapiens
CDTA
-
 Mus musculus
EDTA
-
 Homo sapiens
EDTA
-
 Mus musculus
EGTA
-
 Homo sapiens
EGTA
-
 Mus musculus
leupeptin moderately Homo sapiens
leupeptin moderately Mus musculus
Lys-Arg chloromethyl ketone
-
 Homo sapiens
Lys-Arg chloromethyl ketone
-
 Mus musculus
additional information not: pepstatin Homo sapiens
additional information not: pepstatin Mus musculus
NaCl 600 mM Homo sapiens
NaCl 600 mM Mus musculus
p-hydroxymercuribenzoate
-
 Homo sapiens
p-hydroxymercuribenzoate
-
 Mus musculus
phenylmethanesulfonyl fluoride not Homo sapiens
phenylmethanesulfonyl fluoride not Mus musculus
tosyl-Lys chloromethyl ketone
-
 Homo sapiens
tosyl-Lys chloromethyl ketone
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Mus musculus
additional information
-
 additional information
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Mus musculus
Ca2+ required Homo sapiens
Ca2+ 2.46 mM Mus musculus
Ca2+ 2.46 mM Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 rat somatomammotroph cell line, GH4C1 infected with vaccinia virus recombinants of human PC1
-
Mus musculus
-
 rat somatomammotroph cell line, GH4C1 infected with vaccinia virus recombinants of murine PC1
-

Purification (Commentary)

Purification (Comment) Organism
partial Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Acetyl-Arg-Ser-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus ?
-
 ?
Acetyl-Arg-Ser-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Homo sapiens ?
-
 ?
pGlu-Arg-Thr-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus pGlu-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
pGlu-Arg-Thr-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Homo sapiens pGlu-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
tert-butoxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus tert-butoxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
tert-butoxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Homo sapiens tert-butoxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 80000-85000, mouse, SDS-PAGE Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 acetyl-Arg-Ser-Lys-Arg-4-methylcoumaryl 7-amide Mus musculus
6
-
 acetyl-Arg-Ser-Lys-Arg-4-methylcoumaryl 7-amide Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 6.5 5: about 35% of activity maximum, 6.5: about 15% of activity maximum, mouse, acetyl-Arg-Ser-Lys-Arg-4-methylcoumarin 7-amide Mus musculus
5 6.5 5: about 35% of activity maximum, 6.5: about 15% of activity maximum, mouse, acetyl-Arg-Ser-Lys-Arg-4-methylcoumarin 7-amide Homo sapiens