Any feedback?
Literature summary for 3.4.21.B28 extracted from
- Seprase: An overview of an important matrix serine protease (2008), Biochim. Biophys. Acta, 1784, 1130-1145.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Mus musculus |
| expressed in WI38 cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-(2-aminoethyl)-benzenesulfonylfluoride | 5 mM | Homo sapiens |  |
| 4-(2-aminoethyl)-benzenesulfonylfluoride | 5 mM | Mus musculus | |
| 4-amidino-phenylsulfonylfluoride | 0.5 mM | Homo sapiens | |
| 4-amidino-phenylsulfonylfluoride | 0.5 mM | Mus musculus | |
| diisopropyl fluorophosphate | 0.005 mM | Homo sapiens | |
| diisopropylfluorophosphate | 0.005 mM | Mus musculus | |
| HgCl2 | - |
Homo sapiens | |
| HgCl2 | - |
Mus musculus | |
| N-ethylmaleimide | - |
Homo sapiens | |
| N-ethylmaleimide | - |
Mus musculus | |
| Phenylmethylsulfonylfluoride | 1 mM | Homo sapiens | |
| Phenylmethylsulfonylfluoride | 1 mM | Mus musculus | |
| Talabostat | - |
Homo sapiens | |
| Talabostat | - |
Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.029 | - |
L-Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Gln-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg | recombinant enzyme | Homo sapiens | |
| 0.2 | - |
Gly-Pro-7-amido-4-trifluoromethylcoumarin | Km above 0.2 mM, wild type enzyme | Homo sapiens | |
| 0.2 | - |
L-Ala-Pro-7-amido-4-trifluoromethylcoumarin | Km above 0.2 mM, wild type enzyme | Homo sapiens | |
| 0.25 | - |
L-Ala-Pro-7-amido-4-trifluoromethylcoumarin | recombinant enzyme | Mus musculus | |
| 0.323 | - |
L-Ala-Pro-7-amido-4-trifluoromethylcoumarin | recombinant enzyme | Homo sapiens | |
| 1 | - |
Gly-Pro-7-amido-4-methylcoumarin | Km above 1.0 mM, wild type enzyme | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Gallus gallus | 16020 | - |
| membrane | - |
Bos taurus | 16020 | - |
| membrane | - |
Homo sapiens | 16020 | - |
| membrane | - |
Mus musculus | 16020 | - |
| membrane | - |
Xenopus laevis | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 95000 | - |
recombinant enzyme | Homo sapiens |
| 96000 | - |
- |
Bos taurus |
| 97000 | - |
2 * 97000 | Gallus gallus |
| 97000 | - |
2 * 97000, wild type enzyme | Homo sapiens |
| 160000 | - |
- |
Gallus gallus |
| 170000 | - |
wild type enzyme | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gelatin + H2O | Gallus gallus | - |
? | - |
? | |
| Gelatin + H2O | Bos taurus | - |
? | - |
? | |
| Gelatin + H2O | Homo sapiens | - |
? | - |
? | |
| Gelatin + H2O | Mus musculus | - |
? | - |
? | |
| Gelatin + H2O | Xenopus laevis | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Gallus gallus | - |
- |
- |
| Homo sapiens | Q12884 | - |
- |
| Mus musculus | P97321 | - |
- |
| Xenopus laevis | Q91651 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Gallus gallus |
| glycoprotein | - |
Bos taurus |
| glycoprotein | - |
Homo sapiens |
| glycoprotein | - |
Mus musculus |
| glycoprotein | - |
Xenopus laevis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| S-200 gel filtration, hydroxylapatite column chromatography, Cibacron blue chromatography, and WGA-agarose column chromatography | Homo sapiens |
| S-200 gel filtration, hydroxylapatite column chromatography, Cibacron blue chromatography, and WGA-agarose column chromatography | Mus musculus |
| S-200 gel filtration, hydroxylapatite column chromatography, Cibacron blue chromatography, and WGA-agarose column chromatography | Xenopus laevis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| breast cancer cell line | - |
Homo sapiens | - |
| cervix carcinoma cell | - |
Homo sapiens | - |
| embryo | - |
Gallus gallus | - |
| fibroblast | - |
Homo sapiens | - |
| LOX cell | - |
Homo sapiens | - |
| MDA-MB-231 cell | - |
Homo sapiens | - |
| melanoma cell line | - |
Homo sapiens | - |
| melanoma cell line | - |
Mus musculus | - |
| mesenchyme | transiently expressed in certain normal fetal mesenchymal tissues | Homo sapiens | - |
| additional information | seprase is expressed during wound-healing, normal adult tissues as well as malignant epithelial, neural and haematopoietic cells are generally seprase-negative | Homo sapiens | - |
| serum | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-Gly-Pro + 7-amino-4-methylcoumarin | - |
? | |
| Gelatin + H2O | - |
Gallus gallus | ? | - |
? | |
| Gelatin + H2O | - |
Bos taurus | ? | - |
? | |
| Gelatin + H2O | - |
Homo sapiens | ? | - |
? | |
| Gelatin + H2O | - |
Mus musculus | ? | - |
? | |
| Gelatin + H2O | - |
Xenopus laevis | ? | - |
? | |
| Gly-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Gly-Pro + 7-amino-4-methylcoumarin | - |
? | |
| Gly-Pro-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | Gly-Pro + 7-amino-4-trifluoromethylcoumarin | - |
? | |
| L-Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | L-Ala-Pro + 7-amino-4-trifluoromethylcoumarin | - |
? | |
| L-Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O | - |
Mus musculus | L-Ala-Pro + 7-amino-4-trifluoromethylcoumarin | - |
? | |
| L-Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Gln-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg + H2O | FRET-peptide | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 97000 | Gallus gallus |
| homodimer | 2 * 97000, wild type enzyme | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAPalpha | - |
Gallus gallus |
| FAPalpha | - |
Bos taurus |
| FAPalpha | - |
Homo sapiens |
| FAPalpha | - |
Mus musculus |
| FAPalpha | - |
Xenopus laevis |
| fibroblast activation protein alpha | member of the group type II integral serine proteases, subfamily S9b, with gelatinase and N-terminal post-prolyl amino peptidase activities | Gallus gallus |
| fibroblast activation protein alpha | member of the group type II integral serine proteases, subfamily S9b, with gelatinase and N-terminal post-prolyl amino peptidase activities | Bos taurus |
| fibroblast activation protein alpha | member of the group type II integral serine proteases, subfamily S9b, with gelatinase and N-terminal post-prolyl amino peptidase activities | Homo sapiens |
| fibroblast activation protein alpha | member of the group type II integral serine proteases, subfamily S9b, with gelatinase and N-terminal post-prolyl amino peptidase activities | Mus musculus |
| fibroblast activation protein alpha | member of the group type II integral serine proteases, subfamily S9b, with gelatinase and N-terminal post-prolyl amino peptidase activities | Xenopus laevis |
| seprase | - |
Gallus gallus |
| seprase | - |
Bos taurus |
| seprase | - |
Homo sapiens |
| seprase | - |
Mus musculus |
| seprase | - |
Xenopus laevis |
| surface expressed protease | - |
Gallus gallus |
| surface expressed protease | - |
Bos taurus |
| surface expressed protease | - |
Homo sapiens |
| surface expressed protease | - |
Mus musculus |
| surface expressed protease | - |
Xenopus laevis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Gallus gallus |
| 37 | - |
- |
Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.08 | - |
L-Ala-Pro-7-amido-4-trifluoromethylcoumarin | recombinant enzyme | Homo sapiens | |
| 1.2 | - |
L-Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Gln-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg | recombinant enzyme | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
wild type enzyme | Homo sapiens |
| 7.5 | - |
- |
Bos taurus |
| 7.6 | - |
- |
Gallus gallus |
| 8.5 | - |
recombinant enzyme | Homo sapiens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | isoelectric focusing | - |
5 |
| Bos taurus | isoelectric focusing | - |
5.68 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00004 | - |
- |
Homo sapiens | Talabostat | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
