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Literature summary for 3.4.22.24 extracted from

  • Gohda, E.; Pitot, H.C.
    A new thiol proteinase from rat liver (1981), J. Biol. Chem., 256, 2567-2572.
    View publication on PubMed
Search for more literature for 3.4.22.24

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activation, 4 mM Rattus norvegicus
cysteine activation, 4 mM Rattus norvegicus
DTT activation, 2 mM Rattus norvegicus
EDTA activation Rattus norvegicus
EDTA 2 mM Rattus norvegicus
GSH activation, 4 mM Rattus norvegicus
sulfhydryl compounds activation Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
4,4'-dipyridyl disulfide
-
 Rattus norvegicus
antipain
-
 Rattus norvegicus
iodoacetamide
-
 Rattus norvegicus
iodoacetate
-
 Rattus norvegicus
leupeptin
-
 Rattus norvegicus
additional information no inhibition by native ribonuclease A Rattus norvegicus
N-ethylmaleimide
-
 Rattus norvegicus
Oxidized ribonuclease A tyrosine aminotransferase as substrate Rattus norvegicus
PCMB
-
 Rattus norvegicus
PMSF
-
 Rattus norvegicus
tosyl-L-lysine chloromethyl ketone
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Rattus norvegicus 5764
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 albino male Holtzman rats
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Acid-denatured hemoglobin + H2O
-
 Rattus norvegicus ?
-
 ?
azocasein + H2O
-
 Rattus norvegicus ?
-
 ?
additional information no hydrolysis of synthetic low MW substrates for exopeptidases, e.g. Gly-(L)-Phe-beta-naphthylamide, L-Ser-(L)-Tyr-beta-naphthylamide, L-Leu-beta-naphthylamide, L-Arg-beta-naphthylamide, L-leucinamide, hippuryl-L-arginine, hippuryl-L-phenylalanine, N-benzoyl-L-tyrosine ethyl ester Rattus norvegicus ?
-
 ?
additional information little or no activity at 0øC with glucose 6-phosphate dehydrogenase, native ribonuclease A, native bovine serum albumin, cytochrome c or oxidized insulin B-chain Rattus norvegicus ?
-
 ?
Performic acid-oxidized ribonuclease A + H2O even at 0øC Rattus norvegicus Hydrolyzed performic acid-oxidized ribonuclease A several fragments ?
Tyrosine aminotransferase form I + H2O thereby generating multiple forms of tyrosine aminotransferase Rattus norvegicus Tyrosine aminotransferase form II and III + acidic peptide of MW 4500 peptide enzyme form III: dimer of MW 49000 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
-
-
 assay at, tyrosine aminotransferase as substrate Rattus norvegicus
37
-
 assay at, proteins as substrate Rattus norvegicus