Any feedback?
Literature summary for 3.4.22.24 extracted from
- Cathepsin T (1987), Methods Enzymol., 142, 279-289.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | activation | Rattus norvegicus |  |
| sulfhydryl compounds | activation | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| antipain | - |
Rattus norvegicus | |
| iodoacetamide | - |
Rattus norvegicus | |
| iodoacetate | - |
Rattus norvegicus | |
| leupeptin | - |
Rattus norvegicus | |
| additional information | no inhibition by pepstatin A, soybean trypsin inhibitor | Rattus norvegicus | |
| N-ethylmaleimide | - |
Rattus norvegicus | |
| PCMB | - |
Rattus norvegicus | |
| PMSF | weak | Rattus norvegicus | |
| tosyl-L-lysine chloromethyl ketone | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosome | - |
Rattus norvegicus | 5764 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33500 | - |
gel filtration | Rattus norvegicus |
| 33500 | - |
rat, liver | Rattus norvegicus |
| 35000 | - |
1 * 35000, liver | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Tyrosine aminotransferase + H2O | Rattus norvegicus | involved in generation of isozymic forms of tyrosine transferase | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| kidney | Rattus norvegicus |
| liver | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HTC cell | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| small intestine | - |
Rattus norvegicus | - |
| spleen | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, in 50 mM sodium phosphate buffer, pH 6.5, 25 mM NaCl, 12.5% glycerol, 1 mM DTT, at least 2 months | Rattus norvegicus |
| 4°C, at somewhat higher ionic strength than 50 mM sodium phosphate, pH 6.5, 25 mM NaCl, 12.5% glycerol, 1 mM DTT, inactivation within 1 week | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Acid-denatured hemoglobin + H2O | - |
Rattus norvegicus | ? | - |
? | |
| azocasein + H2O | - |
Rattus norvegicus | ? | - |
? | |
| additional information | cytosolic or mitochondrial forms of aspartate aminotransferase | Rattus norvegicus | ? | - |
? | |
| additional information | Nalpha-benzoyl-(DL)-Arg-beta-naphthylamide | Rattus norvegicus | ? | - |
? | |
| additional information | little or no activity at 0øC with glucose 6-phosphate dehydrogenase, native ribonuclease A, native bovine serum albumin, cytochrome c or oxidized insulin B-chain | Rattus norvegicus | ? | - |
? | |
| Performic acid-oxidized ribonuclease A + H2O | - |
Rattus norvegicus | Hydrolyzed performic acid-oxidized ribonuclease A | - |
? | |
| Tyrosine aminotransferase + H2O | involved in generation of isozymic forms of tyrosine transferase | Rattus norvegicus | ? | - |
? | |
| Tyrosine aminotransferase form I + H2O | enzyme converses native larger subunit to smaller subunit | Rattus norvegicus | Tyrosine aminotransferase form II and III + acidic peptide of MW 4500 peptide | - |
? | |
| Tyrosine aminotransferase form I + H2O | thereby generating multiple forms of tyrosine aminotransferase | Rattus norvegicus | Tyrosine aminotransferase form II and III + acidic peptide of MW 4500 peptide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | SDS-PAGE | Rattus norvegicus |
| monomer | 1 * 35000, liver | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
15 min, pH 6.5, 50% loss of activity | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
0°C | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | active in this range, negligible below pH 5 and above pH 8 | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
15 min, 37°C, 50% loss of activity | Rattus norvegicus |
| 7 | - |
above, 0°C, 1.25% glycerol, rapid loss of activity | Rattus norvegicus |
| 7.8 | - |
20 min, 0°C, inactivation | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
