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Literature summary for 3.4.22.28 extracted from

  • Ye, S.; Xia, H.; Dong, C.; Cheng, Z.; Xia, X.; Zhang, J.; Zhou, X.; Hu, Y.
    Identification and characterization of Iflavirus 3C-like protease processing activities (2012), Virology, 428, 136-145.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Ectropis obliqua virus

Protein Variants

Protein Variants Comment Organism
C238A inactive Ectropis obliqua virus
D2299A the mutant shows much weaker autocatalytic processing activity compared to the wild type Ectropis obliqua virus
H2261A inactive Ectropis obliqua virus

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzenesulfonate strong inhibition at 0.05 mM Ectropis obliqua virus
Cu2+ 5 mM decreases protease activity remarkably relative to the level of activity before the extra cations were added Ectropis obliqua virus
Hg2+ 5 mM decreases protease activity remarkably relative to the level of activity before the extra cations were added Ectropis obliqua virus
methyl methanethiosulfonate almost complete inhibition at 0.05 mM Ectropis obliqua virus
N-ethylmaleimide almost complete inhibition at 0.05 mM Ectropis obliqua virus
Na+ the enzyme is strongly inhibited by 200 mM Na+ Ectropis obliqua virus
phenylmethylsulfonyl fluoride
-
Ectropis obliqua virus
Zn2+ 5 mM decreases protease activity remarkably relative to the level of activity before the extra cations were added Ectropis obliqua virus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mn2+ or Ca2+ do not affect protease activity Ectropis obliqua virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
x * 34000, SDS-PAGE Ectropis obliqua virus

Organism

Organism UniProt Comment Textmining
Ectropis obliqua virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Ectropis obliqua virus

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Ectropis obliqua virus

Synonyms

Synonyms Comment Organism
3C-like protease
-
Ectropis obliqua virus
3CL
-
Ectropis obliqua virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
-
Ectropis obliqua virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Ectropis obliqua virus