Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Ectropis obliqua virus |
Protein Variants | Comment | Organism |
---|---|---|
C238A | inactive | Ectropis obliqua virus |
D2299A | the mutant shows much weaker autocatalytic processing activity compared to the wild type | Ectropis obliqua virus |
H2261A | inactive | Ectropis obliqua virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzenesulfonate | strong inhibition at 0.05 mM | Ectropis obliqua virus | |
Cu2+ | 5 mM decreases protease activity remarkably relative to the level of activity before the extra cations were added | Ectropis obliqua virus | |
Hg2+ | 5 mM decreases protease activity remarkably relative to the level of activity before the extra cations were added | Ectropis obliqua virus | |
methyl methanethiosulfonate | almost complete inhibition at 0.05 mM | Ectropis obliqua virus | |
N-ethylmaleimide | almost complete inhibition at 0.05 mM | Ectropis obliqua virus | |
Na+ | the enzyme is strongly inhibited by 200 mM Na+ | Ectropis obliqua virus | |
phenylmethylsulfonyl fluoride | - |
Ectropis obliqua virus | |
Zn2+ | 5 mM decreases protease activity remarkably relative to the level of activity before the extra cations were added | Ectropis obliqua virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mn2+ or Ca2+ do not affect protease activity | Ectropis obliqua virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, SDS-PAGE | Ectropis obliqua virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ectropis obliqua virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Ectropis obliqua virus |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, SDS-PAGE | Ectropis obliqua virus |
Synonyms | Comment | Organism |
---|---|---|
3C-like protease | - |
Ectropis obliqua virus |
3CL | - |
Ectropis obliqua virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
- |
Ectropis obliqua virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Ectropis obliqua virus |