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Literature summary for 3.4.22.29 extracted from
- Picornains 2A and 3C (1994), Methods Enzymol., 244, 583-595.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in HeLa cells | Enterovirus C |
| expressed in HeLa cells | enterovirus |
| expressed in HeLa cells | coxsackievirus |
| expressed in HeLa cells | picornaviridae |
| expressed in HeLa cells | Human rhinovirus sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chymostatin | - |
coxsackievirus |  |
| chymostatin | - |
enterovirus | |
| chymostatin | - |
Enterovirus C | |
| chymostatin | - |
Human rhinovirus sp. | |
| chymostatin | - |
picornaviridae | |
| Elastinal | - |
coxsackievirus | |
| Elastinal | - |
enterovirus | |
| Elastinal | - |
Enterovirus C | |
| Elastinal | - |
Human rhinovirus sp. | |
| Elastinal | - |
picornaviridae | |
| Hg2+ | - |
coxsackievirus | |
| Hg2+ | - |
enterovirus | |
| Hg2+ | - |
Enterovirus C | |
| Hg2+ | - |
Human rhinovirus sp. | |
| Hg2+ | - |
picornaviridae | |
| iodoacetamide | - |
coxsackievirus | |
| iodoacetamide | - |
enterovirus | |
| iodoacetamide | - |
Enterovirus C | |
| iodoacetamide | - |
Human rhinovirus sp. | |
| iodoacetamide | - |
picornaviridae | |
| additional information | no inhibition by trans-epoxysuccinyl-L-leucylamido(4-guanidino)-butane (i.e. E-64) | coxsackievirus | |
| additional information | no inhibition by trans-epoxysuccinyl-L-leucylamido(4-guanidino)-butane (i.e. E-64) | enterovirus | |
| additional information | no inhibition by trans-epoxysuccinyl-L-leucylamido(4-guanidino)-butane (i.e. E-64) | Enterovirus C | |
| additional information | no inhibition by trans-epoxysuccinyl-L-leucylamido(4-guanidino)-butane (i.e. E-64) | Human rhinovirus sp. | |
| additional information | no inhibition by trans-epoxysuccinyl-L-leucylamido(4-guanidino)-butane (i.e. E-64) | picornaviridae | |
| NEM | - |
coxsackievirus | |
| NEM | - |
enterovirus | |
| NEM | - |
Enterovirus C | |
| NEM | - |
Human rhinovirus sp. | |
| NEM | - |
picornaviridae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
Thr-Arg-Pro-Ile-Ile-Thr-Thr-Ala-Gly-Pro-Ser-Asp-Met-Val-Tyr | human rhinovirus | Human rhinovirus sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | - |
Enterovirus C | |
| additional information | - |
enterovirus | |
| additional information | - |
coxsackievirus | |
| additional information | - |
picornaviridae | |
| additional information | human rhinovirus HRV 2 enzyme is largely unaffected by ionic strength | Human rhinovirus sp. |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
- |
Human rhinovirus sp. |
| 17000 | - |
coxsackievirus B4, gel filtration | coxsackievirus |
| 30000 | - |
human rhinovirus HRV 2, gel filtration | Human rhinovirus sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| picornavirus polyprotein + H2O | picornaviridae | intramolecular reaction, processing begins before synthesis of the polyprotein is complete, cleavage separates capsid protein precursor and noncapsid protein precursor | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| coxsackievirus | - |
B4 | - |
| enterovirus | - |
bovine enterovirus | - |
| Enterovirus C | - |
- |
- |
| Human rhinovirus sp. | - |
serotypes 2 | - |
| Human rhinovirus sp. | - |
serotypes 2 (HRV 2) | - |
| picornaviridae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| as a fusion protein | coxsackievirus |
| from infected HeLa cells | Enterovirus C |
| HRV 2 | Human rhinovirus sp. |
| recombinant enzyme as fusion protein | Human rhinovirus sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | synthesized in cells following infection by picornaviruses | Enterovirus C | - |
| additional information | synthesized in cells following infection by picornaviruses | enterovirus | - |
| additional information | synthesized in cells following infection by picornaviruses | coxsackievirus | - |
| additional information | synthesized in cells following infection by picornaviruses | picornaviridae | - |
| additional information | synthesized in cells following infection by picornaviruses | Human rhinovirus sp. | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | cleavage specificity | Enterovirus C | ? | - |
? | |
| additional information | cleavage specificity | enterovirus | ? | - |
? | |
| additional information | cleavage specificity | coxsackievirus | ? | - |
? | |
| additional information | cleavage specificity | picornaviridae | ? | - |
? | |
| additional information | cleavage specificity | Human rhinovirus sp. | ? | - |
? | |
| additional information | the primary cleavage in cardio- and aphthovirus occurs between 2A and 2B (cleavage site: Gly-Pro in-Asn-Pro-Gly-Pro-) | Enterovirus C | ? | - |
? | |
| additional information | the primary cleavage in cardio- and aphthovirus occurs between 2A and 2B (cleavage site: Gly-Pro in-Asn-Pro-Gly-Pro-) | enterovirus | ? | - |
? | |
| additional information | the primary cleavage in cardio- and aphthovirus occurs between 2A and 2B (cleavage site: Gly-Pro in-Asn-Pro-Gly-Pro-) | coxsackievirus | ? | - |
? | |
| additional information | the primary cleavage in cardio- and aphthovirus occurs between 2A and 2B (cleavage site: Gly-Pro in-Asn-Pro-Gly-Pro-) | picornaviridae | ? | - |
? | |
| additional information | the primary cleavage in cardio- and aphthovirus occurs between 2A and 2B (cleavage site: Gly-Pro in-Asn-Pro-Gly-Pro-) | Human rhinovirus sp. | ? | - |
? | |
| picornavirus polyprotein + H2O | immediately after protease 2A has been synthesized the Tyr-Gly pair between P1 and P2 of the nascent rhinovirus or enterovirus chain is cleaved intramolecularly | Enterovirus C | hydrolyzed picornavirus polyprotein | - |
? | |
| picornavirus polyprotein + H2O | immediately after protease 2A has been synthesized the Tyr-Gly pair between P1 and P2 of the nascent rhinovirus or enterovirus chain is cleaved intramolecularly | enterovirus | hydrolyzed picornavirus polyprotein | - |
? | |
| picornavirus polyprotein + H2O | immediately after protease 2A has been synthesized the Tyr-Gly pair between P1 and P2 of the nascent rhinovirus or enterovirus chain is cleaved intramolecularly | coxsackievirus | hydrolyzed picornavirus polyprotein | - |
? | |
| picornavirus polyprotein + H2O | immediately after protease 2A has been synthesized the Tyr-Gly pair between P1 and P2 of the nascent rhinovirus or enterovirus chain is cleaved intramolecularly | picornaviridae | hydrolyzed picornavirus polyprotein | - |
? | |
| picornavirus polyprotein + H2O | immediately after protease 2A has been synthesized the Tyr-Gly pair between P1 and P2 of the nascent rhinovirus or enterovirus chain is cleaved intramolecularly | Human rhinovirus sp. | hydrolyzed picornavirus polyprotein | - |
? | |
| picornavirus polyprotein + H2O | cleavage between C-terminus of VP1 and N-terminus of picornain 2A | Enterovirus C | hydrolyzed picornavirus polyprotein | - |
? | |
| picornavirus polyprotein + H2O | cleavage between C-terminus of VP1 and N-terminus of picornain 2A | enterovirus | hydrolyzed picornavirus polyprotein | - |
? | |
| picornavirus polyprotein + H2O | cleavage between C-terminus of VP1 and N-terminus of picornain 2A | coxsackievirus | hydrolyzed picornavirus polyprotein | - |
? | |
| picornavirus polyprotein + H2O | cleavage between C-terminus of VP1 and N-terminus of picornain 2A | picornaviridae | hydrolyzed picornavirus polyprotein | - |
? | |
| picornavirus polyprotein + H2O | cleavage between C-terminus of VP1 and N-terminus of picornain 2A | Human rhinovirus sp. | hydrolyzed picornavirus polyprotein | - |
? | |
| picornavirus polyprotein + H2O | cleaves intermolecularly at a second site in the 3D protein between 3C' and 3D' | Enterovirus C | hydrolyzed picornavirus polyprotein | - |
? | |
| picornavirus polyprotein + H2O | cleaves intermolecularly at a second site in the 3D protein between 3C' and 3D' | enterovirus | hydrolyzed picornavirus polyprotein | - |
? | |
| picornavirus polyprotein + H2O | cleaves intermolecularly at a second site in the 3D protein between 3C' and 3D' | coxsackievirus | hydrolyzed picornavirus polyprotein | - |
? | |
| picornavirus polyprotein + H2O | cleaves intermolecularly at a second site in the 3D protein between 3C' and 3D' | picornaviridae | hydrolyzed picornavirus polyprotein | - |
? | |
| picornavirus polyprotein + H2O | cleaves intermolecularly at a second site in the 3D protein between 3C' and 3D' | Human rhinovirus sp. | hydrolyzed picornavirus polyprotein | - |
? | |
| picornavirus polyprotein + H2O | intramolecular reaction, processing begins before synthesis of the polyprotein is complete, cleavage separates capsid protein precursor and noncapsid protein precursor | picornaviridae | ? | - |
? | |
| Thr-Arg-Pro-Ile-Ile-Thr-Thr-Ala-Gly-Pro-Ser-Asp-Met-Val-Tyr + H2O | - |
Human rhinovirus sp. | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Enterovirus C |
| 7 | 8 | - |
enterovirus |
| 7 | 8 | - |
coxsackievirus |
| 7 | 8 | - |
picornaviridae |
| 7 | 8 | - |
Human rhinovirus sp. |
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