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Literature summary for 3.4.22.3 extracted from
- Purification, catalytic, kinetic and thermodynamic characteristics of a novel ficin from Ficus johannis (2017), Biocatal. Agricult. Biotechnol., 10, 360-366 .
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0137 | - |
casein | pH 7.0, 60°C | Ficus johannis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| BaCl2 | 5 mM, 9% inhibition | Ficus johannis | |
| CaCl2 | 5 mM, 31% inhibition | Ficus johannis | |
| CuCl2 | 5 mM, complete inhibition | Ficus johannis | |
| HgCl2 | 5 mM, complete inhibition | Ficus johannis | |
| MgCl2 | 5 mM, 37% inhibition | Ficus johannis | |
| NiCl2 | 5 mM, 88% inhibition | Ficus johannis | |
| PbCl2 | 5 mM, complete inhibition | Ficus johannis | |
| ZnCl2 | 5 mM, 74% inhibition | Ficus johannis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
SDS-PAGE | Ficus johannis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ficus johannis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ficus johannis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| latex | - |
Ficus johannis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Ficus johannis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25000, SDS-PAGE | Ficus johannis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Ficus johannis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | 30°C: about 50% of maximal activity, 80°C: about 60% of maximal activity | Ficus johannis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 32.9 | - |
casein | pH 7.0, 60°C | Ficus johannis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Ficus johannis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | pH 4.0: about 55% of maximal activity, pH 9.0: about 70% of maximal activity | Ficus johannis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2401 | - |
casein | pH 7.0, 60°C | Ficus johannis | |
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