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Literature summary for 3.4.22.34 extracted from

  • Alim, M.A.; Tsuji, N.; Miyoshi, T.; Islam, M.K.; Huang, X.; Hatta, T.; Fujisaki, K.
    HlLgm2, a member of asparaginyl endopeptidases/legumains in the midgut of the ixodid tick Haemaphysalis longicornis, is involved in blood-meal digestion (2008), J. Insect Physiol., 54, 573-585.
    View publication on PubMed
Search for more literature for 3.4.22.34

Activating Compound

Activating Compound Comment Organism Structure
additional information endogenous HlLgm2 expression in the midgut that is upregulated by the blood-feeding process is shown in all the developmental stages Haemaphysalis longicornis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Haemaphysalis longicornis

Inhibitors

Inhibitors Comment Organism Structure
antipain papain-like cysteine proteinase inhibitor, 28.77% inhibition at 0.1 mM concentration relative to control Haemaphysalis longicornis
Ca2+ moderate inhibition Haemaphysalis longicornis
Co2+ strong inhibition Haemaphysalis longicornis
Cu2+ strong inhibition Haemaphysalis longicornis
cystatin from egg white, 62.3% inhibition at 0.003 mM concentration relative to control Haemaphysalis longicornis
E-64 papain-like cysteine proteinase inhibitor, 7.51% inhibition at 0.3 mM concentration relative to control Haemaphysalis longicornis
Fe2+ strong inhibition Haemaphysalis longicornis
iodoacetamide 100% inhibition at 3 mM concentration relative to control Haemaphysalis longicornis
leupeptin papain-like cysteine proteinase inhibitor, 3.9% inhibition at 0.3 mM concentration relative to control Haemaphysalis longicornis
Mg2+ moderate inhibition Haemaphysalis longicornis
Mn2+ moderate inhibition Haemaphysalis longicornis
N-ethylmaleimide 100% inhibition at 3 mM concentration relative to control Haemaphysalis longicornis
Ni2+ strong inhibition Haemaphysalis longicornis
pepstatin A aspartic peptidase inhibitor has minimal effects on the enzyme activity, 12.22% inhibition at 0.3 mM concentration relative to control Haemaphysalis longicornis
PMSF serine peptidase inhibitor, 13.84% inhibition at 5 mM concentration relative to control Haemaphysalis longicornis
Zn2+ moderate inhibition Haemaphysalis longicornis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 endogenous HlLgm2, predicted and confirmed by SDS-PAGE and immunoblot analysis Haemaphysalis longicornis
42500
-
 His-tagged rHlLgm2, determined by SDS-PAGE Haemaphysalis longicornis
47490
-
 predicted preprotein Haemaphysalis longicornis
49630
-
 predicted preproprotein Haemaphysalis longicornis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
bovine albumin + H2O Haemaphysalis longicornis
-
 peptides containing C-terminal Asn
-
 ?
bovine haemoglobin + H2O Haemaphysalis longicornis exhibited strict specificity for the asparaginyl bonds on the carboxy-terminal side of a peptide peptides containing C-terminal Asn
-
 ?
additional information Haemaphysalis longicornis enzyme is involved in host blood-meal digestion ?
-
 ?

Organism

Organism UniProt Comment Textmining
Haemaphysalis longicornis A9CQC1
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein asparagine-linked glycosylation Haemaphysalis longicornis
proteolytic modification the values expected on the basis of the predicted MW indicating that post-translational processing of HlLgm2 occurred during biosynthesis to form a mature protein through removal of 9 kDa peptides from the precursor protein, putative C-terminal autocleavage at site Asn364-365 Haemaphysalis longicornis

Purification (Commentary)

Purification (Comment) Organism
recombinant protein is purified using Ni-Sepharos under native conditions Haemaphysalis longicornis

Source Tissue

Source Tissue Comment Organism Textmining
adult
-
 Haemaphysalis longicornis
-
larva
-
 Haemaphysalis longicornis
-
midgut
-
 Haemaphysalis longicornis
-
nymph
-
 Haemaphysalis longicornis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000133
-
 hydrolysis of substrate benzyloxycarbonyl-Ala-Ala-Asn-4-methyl-coumaryl-7-amide Haemaphysalis longicornis
0.00041
-
 hydrolysis of substrate acetyl-aspartyl-asparaginyl-leucyl-aspartic acid alpha-(4-methylcoumaryl-7-amide) Haemaphysalis longicornis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-Asp-Asn-Leu-Asp alpha-(4-methylcoumaryl-7-amide) + H2O
-
 Haemaphysalis longicornis Ac-Asp-Asn-Leu-Asp + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O
-
 Haemaphysalis longicornis benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
bovine albumin + H2O
-
 Haemaphysalis longicornis peptides containing C-terminal Asn
-
 ?
bovine haemoglobin + H2O exhibited strict specificity for the asparaginyl bonds on the carboxy-terminal side of a peptide Haemaphysalis longicornis peptides containing C-terminal Asn
-
 ?
additional information enzyme is involved in host blood-meal digestion Haemaphysalis longicornis ?
-
 ?

Synonyms

Synonyms Comment Organism
Asparaginyl endopeptidase
-
 Haemaphysalis longicornis
HlLgm2
-
 Haemaphysalis longicornis
legumain
-
 Haemaphysalis longicornis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Haemaphysalis longicornis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 the rHlLgm2 is more active in neutral to slightly alkaline condition Haemaphysalis longicornis

pI Value

Organism Comment pI Value Maximum pI Value
Haemaphysalis longicornis predicted preprotein
-
 6.7
Haemaphysalis longicornis predicted preproprotein
-
 7.1